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Rabbit Recombinant Monoclonal Collagen II antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples. Cited in 10 publications.

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Images

Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007), expandable thumbnail
  • Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007), expandable thumbnail
  • Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Alternative names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

Recommended products

Rabbit Recombinant Monoclonal Collagen II antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12268
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab239007 is the carrier-free version of Anti-Collagen II antibody [EPR12268] ab188570.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.

Biological function summary

Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.

Pathways

Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.

Associated diseases and disorders

Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007), expandable thumbnail

    Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007)

    Actual band size: 30kDa (possible isoform or fragment)
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen II antibody [EPR12268] ab188570).

    All lanes: Western blot - Anti-Collagen II antibody [EPR12268] (Anti-Collagen II antibody [EPR12268] ab188570) at 1/5000 dilution

    Lane 1: Rat cartilage lysate at 20 µg

    Lane 2: Human cartilage lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa

  • Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007), expandable thumbnail

    Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007)

    Actual band size: 30 kDa (possible isoform or fragment).

    Negative control: liver (PMID: 31553813, PMID: 9121483).

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen II antibody [EPR12268] ab188570).

    All lanes: Western blot - Anti-Collagen II antibody [EPR12268] (Anti-Collagen II antibody [EPR12268] ab188570) at 1/5000 dilution

    Lane 1: Rat articular cartilage lysate at 20 µg

    Lane 2: Rat liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 30 kDa

    Exposure time: 10s

  • Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (ab239007)

    Collagen II western blot using anti-Collagen II antibody [EPR12268] - BSA and Azide free ab239007. Publication image and figure legend from Wu, Y., Zhang, H., et al., 2020, J Transl Med, PubMed 32448279.


    ab239007 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab239007 please see the product overview.

    Expression of KAT6B downstream molecules. qPCR was performed to measure the expression of RUNX2 (a), RUNX3 (b), COL2A1 (c), and COL10A1 (d) in primary chondrocytes from control (N = 10) and CS (N = 13) groups. e Western blot was performed to measure the expression of RUNX2, RUNX3, COL2A1, and COL10A1 in primary chondrocytes from control and CS groups. CS, congenital scoliosis. *p < 0.05

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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