Anti-Collagen II antibody [EPR12268] - BSA and Azide free

• WB

Lab

Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007)

Actual band size : 30kDa (possible isoform or fragment) Blocking and diluting buffer : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188570).

All lanes:

Western blot - Anti-Collagen II antibody [EPR12268] (<a href='/en-us/products/primary-antibodies/collagen-ii-antibody-epr12268-ab188570'>ab188570</a>) at 1/5000 dilution

Lane 1:

Rat cartilage lysate at 20 µg

Lane 2:

Human cartilage lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 141 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007)

Actual band size : 30 kDa (possible isoform or fragment).

Negative control : liver (PMID : 31553813, PMID : 9121483).

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188570).

All lanes:

Western blot - Anti-Collagen II antibody [EPR12268] (<a href='/en-us/products/primary-antibodies/collagen-ii-antibody-epr12268-ab188570'>ab188570</a>) at 1/5000 dilution

Lane 1:

Rat articular cartilage lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

false

Exposure time: 10s

• WB

CiteAb

Western blot - Anti-Collagen II antibody [EPR12268] - BSA and Azide free (AB239007)

Collagen II western blot using anti-Collagen II antibody [EPR12268] - BSA and Azide free ab239007. Publication image and figure legend from Wu, Y., Zhang, H., et al., 2020, J Transl Med, PubMed 32448279.

ab239007 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab239007 please see the product overview.

Expression of KAT6B downstream molecules. qPCR was performed to measure the expression of RUNX2 (a), RUNX3 (b), COL2A1 (c), and COL10A1 (d) in primary chondrocytes from control (N = 10) and CS (N = 13) groups. e Western blot was performed to measure the expression of RUNX2, RUNX3, COL2A1, and COL10A1 in primary chondrocytes from control and CS groups. CS, congenital scoliosis. *p < 0.05

false