AB23445

Anti-Collagen III antibody [1E7-D7/Col3]

Name: Anti-Collagen III antibody [1E7-D7/Col3] (ab23445) | Abcam
Brand: Abcam Limited
SKU: AB23445
Price: 400 USD
Availability: InStock
Rating: 4 (5 reviews)

Lab Essentials
What is this?

(5 Reviews)

(35 Publications)

Mouse Monoclonal Collagen III antibody. Suitable for IHC-P and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL3A1.

View Alternative Names

Collagen alpha-1(III) chain, COL3A1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (AB23445)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (AB23445)

Immunohistochemistry (Paraffin-embedded sections) analysis of human bronchus tissue labelling Collagen III with ab23445.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E7-D7/Col3

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human COL3A1.

P02461

Epitope

The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.

Specificity

ab23445 is highly specific for type III Collagen. It has been shown to have no cross reactivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 2.9% Sodium chloride, 0.0268% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.

Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

See full target information COL3A1

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869164

2025

Study of the Effectiveness of Skin Restoration Using a Biopolymer Hydrogel Scaffold with Encapsulated Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marfa N Egorikhina,Lidia B Timofeeva,Yulia P Rubtsova,Ekaterina A Farafontova,Dariya D Linkova,Irina N Charykova,Maksim G Ryabkov,Anna A Ezhevskaya,Ekaterina A Levicheva,Diana Ya Aleynik

Materials today. Bio 32:101790 PubMed40343165

2025

CBD-conjugated BMP-inhibiting exosomes on collagen scaffold dual-target Achilles tendon repair: Synergistic regeneration and heterotopic ossification prevention.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Jiaqiang Huang,Yingjie Mai,Zhiyuan Zhang,Siqi Li,Haofeng Lin,Fuxin Wei,Yang Chen

Analytical cellular pathology (Amsterdam) 2024:6624283 PubMed39624161

2024

N-Acetylcysteine Treats Spinal Cord Injury by Inhibiting Astrocyte Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Chaoxi Qin,Fei Meng,Xiaopeng Han,Xing Guo

Cell reports. Medicine 5:101709 PubMed39226895

2024

Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Wang,Qiang Liu,Ziyan Zhuang,Jianxing Cheng,Wenxiu Zhang,Qiaoling Jiang,Yifei Guo,Ran Li,Xiaojian Lu,Lina Cui,Jiaming Weng,Yanlin Tang,Jingwei Yue,Songzhan Gao,Kai Hong,Jie Qiao,Hui Jiang,Jingtao Guo,Zhe Zhang

Stem cells translational medicine 12:838-848 PubMed37804518

2023

Autologous Airway Basal Cell Transplantation Alleviates Airway Epithelium Defect in Recurrent Benign Tracheal Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Shun Ye,Di-Fei Chen,Ming Liu,Yu-Long Luo,Huan-Jie Chen,Hai-Kang Zeng,Jing-Wei Liu,Yi-Ping Zhu,Xin-Yu Song,Chang-Qin Lin,Zhu-Quan Su,Shi-Yue Li

International journal of molecular sciences 24: PubMed37445612

2023

Adipose Tissue-Derived Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing and Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ho Lee,Yu Jin Won,Hail Kim,Minji Choi,Esther Lee,Bumsik Ryoou,Seok-Geun Lee,Byong Seung Cho

Aesthetic plastic surgery 47:2093-2105 PubMed37022396

2023

Breast Implant Capsule: A Murine Model Comparing Capsular Contracture Susceptibility Among Six Breast Implants Available in the Market.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Bérniz,Francisco Carmona-Torre,Cristina Gómez-Martínez,Leire Fernéndez-Ciriza,Jose Luis Del Pozo,Bernardo Hontanilla

Materials today. Bio 20:100616 PubMed37025556

2023

Photocatalytic oxygen evolution and antibacterial biomimetic repair membrane for diabetes wound repair via HIF1-α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Su,Bing Ye,Ziming Zhang,Qing Gao,Lian Zeng,Yizhou Wan,Wenzhe Sun,Siyue Chen,Daping Quan,Jialin Yu,Xiaodong Guo

Molecular medicine (Cambridge, Mass.) 28:143 PubMed36447136

2022

Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Masateru Nomura,Joseph George,Chieko Hashizume,Takashi Saito,Yoshimichi Ueda,Yasuhito Ishigaki,Mutsumi Tsuchishima,Mikihiro Tsutsumi

Methods in molecular biology (Clifton, N.J.) 2566:113-132 PubMed36152246

2022

Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marie E Esper,Kasun Kodippili,Michael A Rudnicki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen III antibody [1E7-D7/Col3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (AB23445)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (35)

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