Anti-Collagen III antibody (ab7778) is a rabbit polyclonal antibody detecting Collagen III in Western Blot, IP, IHC-P, ICC/IF, ELISA. Suitable for Cow, Human, Rat.
- Over 640 publications
- Trusted since 2002
View Alternative Names
Collagen alpha-1(III) chain, COL3A1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)
Immunohistochemistry of ab7778. Tissue : right lobe of the liver section. A : Central Vein (CV) fibrosis, B : Non-fibrotic CV, C : Perisinusodial fibrosis, D : Non-fibrotic area, E : Protat tract fibrosis, F : Septal fibrosis (arrow). Fixation : formalin fixed paraffin embedded. Antigen retrieval : not required. Primary antibody : Anti-collagen type I at 1 : 500 for 4°C for 24hr. Secondary antibody : Peroxidase biotin-streptavidin rabbit secondary antibody at 1 : 10,000 for 45 min at RT. Localization : Anti-collagen type III is intra and extracellular. Staining : 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)
ab7778 at 1 : 400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 (ab64214) at 99-100°C - 20 minutes for antigen retrieval.
- IHC-P
AbReview25831****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)
ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.
Image courtesy of Rudolf Jung by Abreview.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)
ab53088 staining Human skin (ab30166). Staining is localised to the extracellular matrix.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
- WB
Unknown
Western blot - Anti-Collagen III antibody (AB7778)
Western Blot produced under denaturing and reducing conditions.
All lanes:
Western blot - Anti-Collagen III antibody (ab7778) at 1/1000 dilution
All lanes:
Human collagen III at 0.1 µg
Predicted band size: 139 kDa
false
Reactivity data
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Anti-Collagen III antibody [EPR17673]
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Product details
Anti-Collagen III antibody (ab7778) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC, ICC/IF, IHC-P, IP, WB in mouse samples.
Anti-Collagen III antibody (ab7778) has been cited over 649 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen III antibody (ab7778) has high sensitivity and specificity.
Anti-Collagen III antibody (Colagen type 3) (ab7778) has 21 independent reviews from customers.
Anti-Collagen III antibody (ab7778) specifically detects Collagen III (UniProt ID: P02461; Molecular weight: 95kDa) and is sold in 100 µg selling sizes.
For more protocol tips, please see: https://www.abcam.com/protocols/collagen
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.
Pathways
Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.
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Publications (649)
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Biomolecules & biomedicine : PubMed38059910
2023
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Acta cirurgica brasileira 38:e385123 PubMed38055393
2023
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Biology direct 18:75 PubMed37957699
2023
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Biomaterials research 27:107 PubMed37904231
2023
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Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 29:1218-1227 PubMed37889023
2023
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Scientific reports 13:18374 PubMed37884575
2023
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Scientific reports 13:17985 PubMed37863933
2023
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Scientific reports 13:17242 PubMed37821476
2023
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Biomedicines 11: PubMed37893040
2023
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International journal of molecular sciences 24: PubMed37762688
2023
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