AB216430

Anti-Collagen III antibody - C-terminal

Lab Essentials
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(2 Publications)

Rabbit Polyclonal Collagen III antibody. C-terminal. Suitable for IHC-P and reacts with Human, Dog samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human COL3A1 aa 1350-1450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Collagen alpha-1(III) chain, COL3A1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody - C-terminal (AB216430)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody - C-terminal (AB216430)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human myocardium tissue labeling Collagen III with ab216430 at 1/400 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody - C-terminal (AB216430)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Dog bladder tissue labeling Collagen III with ab216430 at 1/800 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Dog, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human COL3A1 aa 1350-1450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P02461

Specificity

This antibody is produced with an immunogen contained within the COLFI propeptide region, it will only recognize the full length Collagen III protein and not the mature form.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/800", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/800", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.

Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

See full target information COL3A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40539440

2025

polysaccharide alleviates liver fibrosis through the TGF‑β/Smad signaling pathway and reduces collagen.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Yuan,Xiaojing Liu,Tian Zhou,Zhongguang Zhou,Minghai Gong,Yihang Li

Stem cell research & therapy 13:407 PubMed35941707

2022

Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Xing-Liao Zhang,Shou-Tao Lu,Ning-Yan Zhang,Hai-Jun Zhang,Jing Zhang,Jun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen III antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody - C-terminal (AB216430)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody - C-terminal (AB216430)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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