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Anti-Collagen III antibody [EPR17673] ab184993 is a rabbit monoclonal antibody that is used in Collagen III western blotting, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR17673 is cited in over 110 publications

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993), expandable thumbnail
  • Western blot - Anti-Collagen III antibody [EPR17673] (AB184993), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993), expandable thumbnail
  • Western blot - Anti-Collagen III antibody [EPR17673] (AB184993), expandable thumbnail
  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Species

Mouse

Dilution info

1/30

Notes

-

Species

Rat

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Alternative names

Collagen alpha-1(III) chain, COL3A1

Recommended products

Anti-Collagen III antibody [EPR17673] ab184993 is a rabbit monoclonal antibody that is used in Collagen III western blotting, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR17673 is cited in over 110 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17673

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.

Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Associated diseases and disorders

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)

    Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 3 seconds; Lanes 4-5: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).

    All lanes: Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 20 µg

    Lane 2: Human fetal kidney lysate at 20 µg

    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 5: HaCaT (human keratinocyte cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)

    Exposure time : Lanes 1-3: 30 seconds; Lane 4: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).

    All lanes: Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1: Human skin lysate at 10 µg

    Lane 2: Human skeletal muscle lysate at 10 µg

    Lane 3: Human heart lysate at 10 µg

    Lane 4: A431 (human epidermoid carcinoma cell line) whole cell lysate at 10 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 2 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab184993 IP in HeLa whole cell lysate .

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184993 in HeLa whole cell lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

  • Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Collagen IIIwith ab184993 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution (green).Confocal image showing cytoplasmic staining in PC-12 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)

    Blocking buffer and concentration : 5% NFDM/TBST
    Diluting buffer and concentration : 5% NFDM/TBST

    All lanes: Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Collagen III was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: RAW 264.7 whole cell lysate 10 μg (Input). 

    Lane 2: ab184993 IP in RAW 264.7 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184993 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    Lysate was freshly made and used immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Lane 1: RAW 264.7 whole cell lysate 10 μg (Input). 

    Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3.25s

  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Collagen III was immunoprecipitated from 0.35 mg of PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: PC-12 whole cell lysate 10 μg (Input). 

    Lane 2: ab184993 IP in PC-12 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184993 in PC-12 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    Lysate was freshly made and used immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1: PC-12 whole cell lysate 10 μg (Input). 

    Lane 2:  PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab184993 IP in HeLa whole cell lysate . 

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184993 in HeLa whole cell lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

    All lanes: Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Lane 1: HeLa whole cell lysate 10 µg (Input)

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 5.5s

  • Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (ab184993)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma)  cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. 

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993), expandable thumbnail

    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)

    Exposure time :
    Lanes 1-4: 92 seconds;
    Lanes 5-6: 48 seconds;
    Lane 7: 37 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    In lane 1-4, the lysates were stored at -80°C prior to Western Blotting.

    In lane 5-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 5: C6 (rat glial tumor glial cell), whole cell fresh lysate at 20 µg

    Lane 6: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell fresh lysate at 20 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma), whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

    Exposure time :
    Lanes 1-4: 92 seconds;
    Lanes 5-6: 48 seconds;
    Lane 7: 37 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    In lane 1-4, the lysates were stored at -80°C prior to Western Blotting.
    In lane 5-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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