AB86042

Anti-Collagen IV antibody [1043]

Name: Anti-Collagen IV antibody [1043] (ab86042) | Abcam
Brand: Abcam Limited
SKU: AB86042
Price: 395 USD
Availability: InStock

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(12 Publications)

Mouse Monoclonal COL4A1 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL4A1.

View Alternative Names

Collagen alpha-1(IV) chain, COL4A1

3 Images

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

ab86042 (TCS version) staining Collagen IV in Human kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [1043] (AB86042)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [1043] (AB86042)

Immunohistochemistry on paraffin section of Human skin

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

Immunohistochemistry on frozen section of Human colon with ab86042 (TCS version).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1043

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Human COL4A1.

P02462

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform enzymatic antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "" } } }

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Product details

In IHC, recognizes the extracellular – basement membranes.

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27^th October 2017. The following lots are still unpurified and still in stock as of 27^th October 2017- GR275641-7, GR275641-13, and GR275641-14. Lot numbers other than GR275641-7, GR275641-13, and GR275641-14 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

From tissue culture supernatant

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.

Biological function summary

The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.

Pathways

Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.

Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information COL4A1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 50:940-954 PubMed41015555

2025

[ and NFκB positive feedback loop promotes high glucoseinduced pyroptosis in renal tubular epithelial cells].

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yang,Yang Luo,Zixuan Zhu,Wenbin Tang

International journal of molecular sciences 23: PubMed36012314

2022

Aberrant Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem Cells of a Retinitis Pigmentosa Patient with the PRPF6 Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqin Liang,Feng Tan,Xihao Sun,Zekai Cui,Jianing Gu,Shengru Mao,Hon Fai Chan,Shibo Tang,Jiansu Chen

Nature communications 13:1381 PubMed35296667

2022

Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Capparelli,Timothy J Purwin,McKenna Glasheen,Signe Caksa,Manoela Tiago,Nicole Wilski,Danielle Pomante,Sheera Rosenbaum,Mai Q Nguyen,Weijia Cai,Janusz Franco-Barraza,Richard Zheng,Gaurav Kumar,Inna Chervoneva,Ayako Shimada,Vito W Rebecca,Adam E Snook,Kim Hookim,Xiaowei Xu,Edna Cukierman,Meenhard Herlyn,Andrew E Aplin

Journal of healthcare engineering 2022:2279072 PubMed35126911

2022

lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtu Zhao,Yuanyuan Cui,Fuqing Dong,Wencong Li

BMC endocrine disorders 21:134 PubMed34187448

2021

LncRNA HCP5 knockdown inhibits high glucose-induced excessive proliferation, fibrosis and inflammation of human glomerular mesangial cells by regulating the miR-93-5p/HMGA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wang,Yan Liu,Jian Rong,Kai Wang

Journal of clinical laboratory analysis 35:e23857 PubMed34097306

2021

MiR-221-3p and miR-92a-3p enhances smoking-induced inflammation in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Shen,Huiyu Lu,Guixian Song

Frontiers in genetics 12:662751 PubMed34093655

2021

Omics Profiling of S2P Mutant Fibroblasts as a Mean to Unravel the Pathomechanism and Molecular Signatures of X-Linked Osteogenesis Imperfecta.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Jin Lim,Severin Marfurt,Uschi Lindert,Lennart Opitz,Timothée Ndarugendamwo,Pakeerathan Srikanthan,Martin Poms,Martin Hersberger,Claus-Dieter Langhans,Dorothea Haas,Marianne Rohrbach,Cecilia Giunta

Frontiers in pharmacology 12:647650 PubMed33995063

2021

lncRNA MALAT1 Promotes Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-2355-3p/IL6ST Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haozi Huang,Guowei Zhang,Zhenying Ge

International urology and nephrology 53:2177-2187 PubMed33881703

2021

Mechanism of miR-365 in regulating BDNF-TrkB signal axis of HFD/STZ induced diabetic nephropathy fibrosis and renal function.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhao,Xiaqiu Li,Yang Li,Jiaying Zhu,Yu Sun,Jianli Hong

Technology in cancer research & treatment 19:1533033820973277 PubMed33234027

2020

Overexpression of DDR1 Promotes Migration, Invasion, Though EMT-Related Molecule Expression and COL4A1/DDR1/MMP-2 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xie,Hongchao He,Ning Zhang,Xiaojing Wang,Wenbin Rui,Danfeng Xu,Yu Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen IV antibody [1043]

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [1043] (AB86042)

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [1043] (AB86042)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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