Rabbit Polyclonal COL4A1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 169 publications. Immunogen corresponding to Native Full Length Protein corresponding to Mouse Col4a1.
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- Journal of experimental & clinical cancer research : CR 42:3282023Unveiling the role of osteosarcoma-derived secretome in premetastatic lung remodelling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara F F Almeida,Liliana Santos,Gabriela Sampaio-Ribeiro,Hugo R S Ferreira,Nuno Lima,Rui Caetano,Mónica Abreu,Mónica Zuzarte,Ana Sofia Ribeiro,Artur Paiva,Tânia Martins-Marques,Paulo Teixeira,Rui Almeida,José Manuel Casanova,Henrique Girão,Antero J Abrunhosa,Célia M GomesPubMed 38031171
- Journal of neuroinflammation 20:2512023The novel HS-mimetic, Tet-29, regulates immune cell trafficking across barriers of the CNS during inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTessa Peck,Connor Davis,Georgia Lenihan-Geels,Maddie Griffiths,Sam Spijkers-Shaw,Olga V Zubkova,Anne Camille La FlammePubMed 37915090
- Frontiers in bioengineering and biotechnology 11:12140192023Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominik Hanetseder,Tina Levstek,Andreas Herbert Teuschl-Woller,Julia Katharina Frank,Barbara Schaedl,Heinz Redl,Darja Marolt PresenPubMed 37600321
- Frontiers in immunology 14:10539202023Co-cultures of colon cancer cells and cancer-associated fibroblasts recapitulate the aggressive features of mesenchymal-like colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Strating,Mathijs P Verhagen,Emerens Wensink,Ester Dünnebach,Liza Wijler,Itziar Aranguren,Alberto Sanchez De la Cruz,Niek A Peters,Joris H Hageman,Mirjam M C van der Net,Susanne van Schelven,Jamila Laoukili,Riccardo Fodde,Jeanine Roodhart,Stefan Nierkens,Hugo Snippert,Martijn Gloerich,Inne Borel Rinkes,Sjoerd G Elias,Onno KranenburgPubMed 37261365
- Nature communications 13:64092022Divergent regulation of basement membrane trafficking by human macrophages and cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian C Bahr,Xiao-Yan Li,Tamar Y Feinberg,Long Jiang,Stephen J WeissPubMed 36302921
- eLife 11:2022Continuous sensing of IFNα by hepatic endothelial cells shapes a vascular antimetastatic barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNgoc Lan Tran,Lorena Maria Ferreira,Blanca Alvarez-Moya,Valentina Buttiglione,Barbara Ferrini,Paola Zordan,Andrea Monestiroli,Claudio Fagioli,Eugenia Bezzecchi,Giulia Maria Scotti,Antonio Esposito,Riccardo Leone,Chiara Gnasso,Andrea Brendolan,Luca G Guidotti,Giovanni SitiaPubMed 36281643
- iScience 25:1054152022Single cell sequencing maps skeletal muscle cellular diversity as disease severity increases in dystrophic mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKholoud K Saleh,Haibin Xi,Corey Switzler,Emily Skuratovsky,Matthew A Romero,Peggie Chien,Devin Gibbs,Lily Gane,Michael R Hicks,Melissa J Spencer,April D PylePubMed 36388984
- International journal of molecular sciences 23:2022The VEGF Inhibitor Soluble Fms-like Tyrosine Kinase 1 Does Not Promote AKI-to-CKD Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCleo C L van Aanhold,Angela Koudijs,Kyra L Dijkstra,Ron Wolterbeek,Jan A Bruijn,Cees van Kooten,Hans J BaeldePubMed 36077058
- Journal of immunology (Baltimore, Md. : 1950) 209:1001-10122022Human CD99L2 Regulates a Unique Step in Leukocyte Transmigration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNakisha S Rutledge,Faith T Ogungbe,Richard L Watson,David P Sullivan,William A MullerPubMed 35914838
- British journal of cancer 127:811-8232022Inhibition of PFKFB3 in HER2-positive gastric cancer improves sensitivity to trastuzumab by inducing tumour vessel normalisation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingxing Yao,Zhanke He,Caolitao Qin,Penghao Zhang,Chuyang Sui,Xiangqian Deng,Yuxin Fang,Guoxin Li,Jiaolong ShiPubMed 35637411
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Native Full Length Protein corresponding to Mouse Col4a1. Database link P02463
Consider this alternative
Reactivity data
Select an application| IHC-P | |
|---|---|
| Mouse | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
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Target data
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.
Targets
Collagen alpha-3(IV) chain, Collagen alpha-2(IV) chain, Col4a4
Alternative names
Collagen alpha-1(IV) chain, Col4a1
Recommended products
Rabbit Polyclonal COL4A1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 169 publications. Immunogen corresponding to Native Full Length Protein corresponding to Mouse Col4a1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Mouse Col4a1. Database link P02463
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Ion exchange chromatography (DEAE-Trisacryl).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.
- Biological function summary
The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.
- Pathways
Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.
- Associated diseases and disorders
Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Miller K et al. Islet formation during the neonatal development in mice. PLoS One 4:e7739 (2009). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab19808)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for Collagen IV using ab19808.
Endocrine-cells coated with a layer of extracellular matrix (P1). Immunohistochemical staining for Insulin (green), glucagon (red) and collagen IV (yellow) is shown. Note that intra-islet blood vessels are also associated with the extracellular matrix. Scale bar is 50 μm.
The primary antibodies were detected using different combinations of Cy2, Cy5 and Texas Red-conjugated secondary antibodies and the image was captured via confocal microscopy.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab19808)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labeling Collagen IV with ab19808 at ≥1/500 dilution.
This image is courtesy of a customer review submitted by an anonymous researcher on 26 January 2006.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab19808)
ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.
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