AB19808

Anti-Collagen IV antibody

Name: Anti-Collagen IV antibody (ab19808) | Abcam
Brand: Abcam Limited
SKU: AB19808
Price: 630 USD
Availability: InStock
Rating: 5 (24 reviews)

(24 Reviews)

(212 Publications)

Anti-Collagen IV antibody (ab19808) is a rabbit polyclonal antibody detecting Collagen IV in IHC-P. Suitable for Mouse.

- Over 160 publications
- Trusted since 2005

View Alternative Names

Collagen alpha-1(IV) chain, Col4a1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labeling Collagen IV with ab19808 at ≥1/500 dilution.

IHC-P

AbReview4004****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.

This image is courtesy of an Abreview submitted by an anonymous researcher on 26 January 2006.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for Collagen IV using ab19808.

Endocrine-cells coated with a layer of extracellular matrix (P1). Immunohistochemical staining for Insulin (green), glucagon (red) and collagen IV (yellow) is shown. Note that intra-islet blood vessels are also associated with the extracellular matrix. Scale bar is 50 μm.

The primary antibodies were detected using different combinations of Cy2, Cy5 and Texas Red-conjugated secondary antibodies and the image was captured via confocal microscopy.

Miller K et al. Islet formation during the neonatal development in mice. PLoS One 4:e7739 (2009). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Mouse Col4a1.

P02463

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Collagen IV antibody (ab19808) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Mouse samples.

Trusted by the scientific community
Anti-Collagen IV (ab19808) was first used in a scientific publication in 2005 and has been cited over 160 times in peer-reviewed journals.

Reviewed by scientists
Anti-Collagen IV (ab19808) has over 20 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Ion exchange chromatography (DEAE-Trisacryl).

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.

Biological function summary

The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.

Pathways

Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.

Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information Col4a1

Additional targets

,Collagen alpha-2(IV) chain,Col4a4

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Publications (212)

Recent publications for all applications. Explore the full list and refine your search

eLife 14: PubMed40637239

2025

NR2F2 is required in the embryonic testis for fetal Leydig cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Aitana Perea-Gomez,Natividad Bellido Carreras,Magali Dhellemmes,Furong Tang,Coralie Le Gallo,Marie-Christine Chaboissier

World journal of diabetes 16:99473 PubMed40487605

2025

Effect of ranibizumab on diabetic retinopathy the vascular endothelial growth factor/STAT3/glial fibrillary acidic protein pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Ting Lin,Jian Tan,Yu-Lin Tao,Wei-Wen Hu,Yi-Cang Wang,Jing Huang,Qiong Zhou,Ang Xiao

CNS neuroscience & therapeutics 31:e70409 PubMed40415486

2025

Impact of NA-1 on Pericyte-Driven Vasoconstriction and Its Role in No-Reflow During Cerebral Ischemia-Reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxuan Yang,Jiahui Zhao,Hao Tian,Ximing Nie,Lina Zheng,Xin Liu,Zheng Z Wei,Yuchuan Ding,Liping Liu

The EMBO journal 44:3266-3300 PubMed40216939

2025

Fibroblast hierarchy dynamics during mammary gland morphogenesis and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Pascual,Jinming Cheng,Amelia H De Smet,Bianca D Capaldo,Minhsuang Tsai,Somayeh Kordafshari,François Vaillant,Xiaoyu Song,Göknur Giner,Michael J G Milevskiy,Felicity C Jackling,Bhupinder Pal,Toby Dite,Jumana Yousef,Laura F Dagley,Gordon K Smyth,Naiyang Fu,Geoffrey J Lindeman,Yunshun Chen,Jane E Visvader

Reproductive and developmental medicine 9:11-21 PubMed40162300

2025

deficiency partially restores defects in -deficient mouse corpus luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehuan Li,Ahmed E El Zowalaty,Jonathan Matthew Hancock,Zidao Wang,Taylor Elijah Martin,Tingjie Zhan,Yingzheng Wang,Christian Lee Andersen,Suvitha Viswanathan,Jaymie Bromfield,Venkata Abhigna Atluri,Karly Rae Kallish,Hope Nicole Grismer,Shuo Xiao,Xiaoqin Ye

Investigative ophthalmology & visual science 66:39 PubMed40105819

2025

Targeted siRNA Delivery Against RUNX1 Via tFNA: Inhibiting Retinal Neovascularization and Restoring Vessels Through Dll4/Notch1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Zhou,Xiaoxiao Xu,Qiong Wang,Yanting Lai,Linyan Zhang,Yunfeng Lin,Xiaoyan Ding,Limei Sun

IBRO neuroscience reports 18:16-30 PubMed39816479

2025

Effect of metabolic disorders on reactive gliosis and glial scarring at the early subacute phase of stroke in a mouse model of diabetes and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Clain,David Couret,Matthieu Bringart,Olivier Meilhac,Christian Lefebvre d'Hellencourt,Nicolas Diotel

Reproductive and developmental medicine 8:197-205 PubMed39624425

2024

Exogenous estrogen partially rescues progesterone deficiency and autophagosome enlargement in mouse model with lysosomal storage disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Zidao Wang,Yuehuan Li,Christian L Andersen,Ahmed E El Zowalaty,Jonathan M Hancock,Taylor E Martin,Elizabeth W Howerth,Suvitha Viswanathan,Haeyeun Byun,Xiaoqin Ye

eLife 13: PubMed39453386

2024

GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver health.

Applications

Unspecified application

Species

Unspecified reactive species

Dianyuan Zhao,Ziwei Huang,Xiaoyu Li,Huan Wang,Qingwei Hou,Yuyao Wang,Fang Yan,Wenting Yang,Di Liu,Shaoqiong Yi,Chunguang Han,Yanan Hao,Li Tang

Brain and behavior 14:e70036 PubMed39295106

2024

Neuroprotective effects of salvianolic acids combined with Panax notoginseng saponins in cerebral ischemia/reperfusion rats concerning the neurovascular unit and trophic coupling.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyang Chen,Zhen Liu,Lei Zhao,Zhuangzhuang Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB19808)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (212)

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