AB235296

Anti-Collagen IV antibody

Name: Anti-Collagen IV antibody (ab235296) | Abcam
Brand: Abcam Limited
SKU: AB235296
Price: 420 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal COL4A1 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

Collagen alpha-1(IV) chain, Col4a1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Immunohistochemical analysis of mouse placenta tissue labeling Collagen IV with ab235296 at 1/100 dilution for 30 mins at RT, followed by a donkey anti-goat HRP at 4 μL/ml for 20 mins at RT. Counterstained with hematoxylin. HIER was performed using citrate buffer for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody (AB235296)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody (AB235296)

Immunocytochemical analysis of 4% PFA-fixed mouse NIH/3T3 cells labeling Collagen IV with ab235296 at 15 μg/ml overnight at 4°C, followed by a donkey anti-goat IgG DyLight™ 488 conjugated preabsorbed secondary at 5 μg/ml for 1 hour at RT (green). The nuclear counter stain is DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Immunohistochemical analysis of mouse testis tissue labeling Collagen IV with ab235296 at 1/100 dilution for 30 mins at RT, followed by a donkey anti-goat HRP at 4 μL/ml for 20 mins at RT. Counterstained with hematoxylin. HIER was performed using citrate buffer for 20 mins.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.

Biological function summary

The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.

Pathways

Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.

Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information Col4a1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0322721 PubMed40694571

2025

Busulfan damages spermatogenic function by inducing orchitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Zhao,Kaihui Wu,Shiyuan Xu,Songqi Liu,Kaimin Yuan,Dong Wang

Frontiers in cardiovascular medicine 11:1319164 PubMed38545339

2024

The gene variant governs passive ascending aortic mechanics in the mgΔ mouse model of Marfan syndrome when superimposed to perlecan haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Samar A Tarraf,Rodrigo Barbosa de Souza,Ashley Herrick,Lygia V Pereira,Chiara Bellini

The American journal of pathology 190:1943-1959 PubMed32562655

2020

Acoustic Trauma Causes Cochlear Pericyte-to-Myofibroblast-Like Cell Transformation and Vascular Degeneration, and Transplantation of New Pericytes Prevents Vascular Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Hou,Lingling Neng,Jinhui Zhang,Jing Cai,Xiaohan Wang,Yunpei Zhang,Ivan A Lopez,Xiaorui Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody (AB235296)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB235296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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