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Rabbit Polyclonal COL4A1 antibody. Suitable for IHC-P, IP, ELISA, WB, IF, IHC-FoFr, ICC/IF, IHC-Fr, IHC-FrFl and reacts with Human, Cow, Mouse, Rat, African green monkey, Pig, Dog, Hamster, Chinese hamster, Zebrafish, Syrian hamster samples. Cited in 920 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL4A1.
Alternative names=Collagen alpha-1(IV) chain, COL4A1
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Native Full Length Protein corresponding to Human COL4A1. Database link: P02462
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | ELISA | WB | IF | IHC-FoFr | ICC/IF | IHC-Fr | IHC-FrFl | |
|---|---|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| African green monkey | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Chinese hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Cow | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Dog | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Mammals | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Syrian hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Zebrafish | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/15.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse, Rat, African green monkey, Pig, Dog, Hamster, Chinese hamster, Zebrafish, Syrian hamster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Syrian hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Mouse | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Rat | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Pig | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species African green monkey | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Zebrafish | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Dog | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Cow | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-FrFl
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
Recommended products
Rabbit Polyclonal COL4A1 antibody. Suitable for IHC-P, IP, ELISA, WB, IF, IHC-FoFr, ICC/IF, IHC-Fr, IHC-FrFl and reacts with Human, Cow, Mouse, Rat, African green monkey, Pig, Dog, Hamster, Chinese hamster, Zebrafish, Syrian hamster samples. Cited in 920 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL4A1.
Alternative names=Collagen alpha-1(IV) chain, COL4A1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Native Full Length Protein corresponding to Human COL4A1. Database link: P02462
- Purification technique
Affinity purification Immunogen
- Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Concentration
- Purification notes
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody ab214417.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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