Anti-Collagen IV antibody is a rabbit polyclonal antibody that is used to detect Collagen IV in ELISA, ICC/IF, IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, IP, Western blot. Suitable for African green monkey, Chinese hamster, Cow, Dog, Hamster, Human, Mouse, Pig, Rat, Syrian hamster, Zebrafish samples.
- Cited in over 915 publications
- Tried and trusted by researchers since 2001
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- Biomolecules & biomedicine 24:582-5922024Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNejc Umek,Luka Pušnik,Chiedozie Kenneth Ugwoke,Žiga Šink,Simon Horvat,Jiří Janáček,Erika CvetkoPubMed 37902457
- Frontiers in cardiovascular medicine 10:12578122023Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirill Yu Klyshnikov,Maria A Rezvova,Nikita V Belikov,Tatiana V Glushkova,Evgeny A OvcharenkoPubMed 38094125
- Journal of biomedical research 37:460-4692023ELABELA protects against diabetic kidney disease by activating high glucose-inhibited renal tubular autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiyin Zheng,Lulu Yin,Jing Song,Juan Chen,Wensha Gu,Min Shi,Hong ZhangPubMed 38018421
- Nature communications 14:769620233D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonbin Park,Jae-Seong Lee,Ge Gao,Byoung Soo Kim,Dong-Woo ChoPubMed 38001146
- Nature communications 14:76602023Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Reuter,Laura Hauf,Fabian Imdahl,Rituparno Sen,Ehsan Vafadarnejad,Philipp Fey,Tamara Finger,Nicola G Jones,Heike Walles,Lars Barquist,Antoine-Emmanuel Saliba,Florian Groeber-Becker,Markus EngstlerPubMed 37996412
- Nature communications 14:76192023Mid-old cells are a potential target for anti-aging interventions in the elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun ParkPubMed 37993434
- Journal of tissue engineering 14:204173142311972822023A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J Buckenmeyer,Meena Sukhwani,Aimon Iftikhar,Alexis L Nolfi,Ziyu Xian,Srujan Dadi,Zachary W Case,Sarah R Steimer,Antonio D'Amore,Kyle E Orwig,Bryan N BrownPubMed 38029018
- Frontiers in immunology 14:12594342023Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuhua Yang,Emily Huo,Yongfeng Cai,Zhidan Zhang,Charles Dong,John M Asara,Huidong Shi,Qingqing WeiPubMed 38035106
- Cell death & disease 14:7252023Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYakinthi Chrisochoidou,Rajat Roy,Pooyeh Farahmand,Guadalupe Gonzalez,Jennifer Doig,Lukas Krasny,Ella F Rimmer,Anne E Willis,Marion MacFarlane,Paul H Huang,Neil O Carragher,Alison F Munro,Daniel J Murphy,Kirill Veselkov,Michael J Seckl,Miriam F Moffatt,William O C Cookson,Olivier E PardoPubMed 37938546
- Nature communications 14:68142023Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Kolb,Vasiliki Tsata,Nora John,Kyoohyun Kim,Conrad Möckel,Gonzalo Rosso,Veronika Kurbel,Asha Parmar,Gargi Sharma,Kristina Karandasheva,Shada Abuhattum,Olga Lyraki,Timon Beck,Paul Müller,Raimund Schlüßler,Renato Frischknecht,Anja Wehner,Nicole Krombholz,Barbara Steigenberger,Dimitris Beis,Aya Takeoka,Ingmar Blümcke,Stephanie Möllmert,Kanwarpal Singh,Jochen Guck,Katja Kobow,Daniel WehnerPubMed 37884489
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogens
This product was produced with the following immunogens:
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | ELISA | WB | IF | IHC-FoFr | ICC/IF | IHC-Fr | IHC-FrFl | |
|---|---|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| African green monkey | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Chinese hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Cow | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Dog | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Mammals | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Syrian hamster | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Zebrafish | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/15.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse, Rat, African green monkey, Pig, Dog, Hamster, Chinese hamster, Zebrafish, Syrian hamster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Syrian hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Mouse | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Rat | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Pig | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Hamster | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species African green monkey | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Zebrafish | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Dog | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Cow | Dilution info - | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-FrFl
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Syrian hamster, Mouse, Rat, Pig, Hamster, African green monkey, Zebrafish, Dog, Cow, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.
Alternative names
Collagen alpha-1(IV) chain, COL4A1
Recommended products
Anti-Collagen IV antibody is a rabbit polyclonal antibody that is used to detect Collagen IV in ELISA, ICC/IF, IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, IP, Western blot. Suitable for African green monkey, Chinese hamster, Cow, Dog, Hamster, Human, Mouse, Pig, Rat, Syrian hamster, Zebrafish samples.
- Cited in over 915 publications
- Tried and trusted by researchers since 2001
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
This product was produced with the following immunogens:
- Purification technique
- Affinity purification Immunogen
- Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Concentration
- Purification notes
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Collagen IV antibody (ab6586) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IF, IHC-FoFr, IHC-Fr, IHC-FrFl,
Anti-Collagen IV antibody (ab6586) was first used in a scientific publication in 2004 and has been cited over 920 times in peer reviewed journals. It's performance in IHC and Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen IV antibody (ab6586) has high sensitivity and specificity.
Anti-Collagen IV antibody (ab6586) has 120 independent reviews from customers.
Anti-Collagen IV antibody (ab6586) specifically detects Collagen IV (UniProt ID: P02462; Molecular weight: 145kDa) and is sold in 100 µg selling sizes.
Collagen IV is a key component of the extracellular matrix in tumors, contributing to tissue stiffness and promoting cancer cell invasion. It plays a significant role in tumor progression and metastasis, influencing the behaviour of cancer cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen IV also known as COL4A1 and collagen type 4 plays a significant mechanical role in the structure of the basement membrane. It provides structural support and mechanical stability. Collagen IV has a molecular weight ranging approximately from 160 to 240 kDa. It is expressed in various regions including the kidney lung and vascular tissues where it contributes to the integrity and function of these organs.
- Biological function summary
The primary function of collagen type IV involves forming a scaffold essential for basement membrane assembly and function. It exists as a part of a complex formation typically forming networks with other collagens and proteins. These networks are important for cellular adhesion differentiation migration and filtration processes. Collagen IV interacts with different cell surface receptors helping mediate important cellular signals.
- Pathways
Collagen type 4 is integral to the extracellular matrix and integrin-mediated signaling pathways. It partners with proteins such as laminins and nidogens in these pathways contributing to cell adhesion and migration. This interaction helps regulate various cellular processes including wound healing and tissue repair. By being a part of these pathways collagen IV impacts cellular behavior and tissue homeostasis.
- Associated diseases and disorders
Dysfunction or mutations in collagen IV relate to conditions such as Alport syndrome and Goodpasture's syndrome. Alport syndrome involves mutations in collagen IV leading to kidney dysfunction and hearing loss. In Goodpasture's syndrome collagen IV becomes a target for autoimmune attack resulting in damage to the lungs and kidneys. These diseases illustrate the connection between collagen IV and other proteins like laminin which together contribute to maintaining tissue integrity and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human kidney using rabbit anti-Collagen IV antibody
Paraffin-embedded human kidney tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis with strong staining observed in glomeruli.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human liver using rabbit anti-Collagen IV antibody
Paraffin-embedded human liver tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis strong staining was observed in the sinusoids.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human brain using rabbit anti-Collagen IV antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde fixed human brain (cortex) staining with ab6586 at 1/250 dilution. Secondary antibody used was HRP Goat Agilent anti-mouse/rabbit EnVision Dual Link.
Blocking was done using 5% serum for 15 minutes at 20°C. The sample was incubated with the primary antibody for 1 hour at 20°C with Agilent antibody diluent. Antigen retrieval method was heat mediated Citrate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human renal oncocytoma using rabbit anti-Collagen IV antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded human renal oncocytoma tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests. Counterstained with hematoxylin for 15 seconds
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human skeletal muscle using rabbit anti-Collagen IV antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded human skeletal muscle cells labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of human lung using rabbit anti-Collagen IV antibody
Immunohistochemical analysis formalin-fixed paraffin-embedded human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (ab6586)
Collagen IV immunohistochemistry staining of pig kidney using rabbit anti-Collagen IV antibody
Immunohistochemistry analysis of paraformaldehyde perfusion fixed frozen pig kidney permeabilized with 0.05% tween 20. Stained with ab6586 at 1/200 dilution. Secondary antibody used was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. The nuclei are stained Blue with Hoechst. The Red counter stain was created with a methyl green stain and by imaging with a 555nm excitation filter. Blocking was done with Sea Block for 30 minutes at 22°C.
The sample was incubated with the primary antibody and Sea Block for 16 hours at 4°C. Antigen retrieval method was heat mediated with Citrate Buffer pH 6.0.
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