AB6586

Anti-Collagen IV antibody

Name: Anti-collagen IV antibody - rabbit polyclonal (ab6586) | Abcam
Brand: Abcam Limited
SKU: AB6586
Price: 535 USD
Availability: InStock
Rating: 5 (122 reviews)

(122 Reviews)

(1127 Publications)

Anti-Collagen IV antibody (ab6586) is a rabbit polyclonal antibody detecting Collagen IV in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Pig, Rat, Zebrafish.

- Over 920 publications
- Trusted since 2001

View Alternative Names

Collagen alpha-1(IV) chain, COL4A1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Paraffin-embedded human kidney tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis with strong staining observed in glomeruli.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Paraffin-embedded human liver tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis strong staining was observed in the sinusoids.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human skeletal muscle cells labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : cells surrounded by collagen IV fibers. Counterstained with hematoxylin for 15 seconds. Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human renal oncocytoma tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : dense collagen IV positive membranes surrounding tumor cell nests. Counterstained with hematoxylin for 15 seconds Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemical analysis formalin-fixed paraffin-embedded human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : basement membranes and vessels. Counterstained with hematoxylin for 15 seconds. Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

IHC-P

AbReview80282****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde fixed human brain (cortex) staining with ab6586 at 1/250 dilution. Secondary antibody used was HRP Goat Agilent anti-mouse/rabbit EnVision Dual Link. Blocking was done using 5% serum for 15 minutes at 20°C. The sample was incubated with the primary antibody for 1 hour at 20°C with Agilent antibody diluent. Antigen retrieval method was heat mediated Citrate

This image is courtesy of an anonymous Abreview

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (AB6586)

IHC-FoFr

AbReview74548****

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry analysis of paraformaldehyde perfusion fixed frozen pig kidney permeabilized with 0.05% tween 20. Stained with ab6586 at 1/200 dilution. Secondary antibody used was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. The nuclei are stained Blue with Hoechst. The Red counter stain was created with a methyl green stain and by imaging with a 555nm excitation filter. Blocking was done with Sea Block for 30 minutes at 22°C. The sample was incubated with the primary antibody and Sea Block for 16 hours at 4°C. Antigen retrieval method was heat mediated with Citrate Buffer pH 6.0.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Hamster, Syrian hamster, Cow, Mouse, Dog, Zebrafish, Rat, Chinese hamster, African green monkey, Pig

Applications

IHC-FoFr, IHC-FrFl, IHC-P, WB, IF, IP, ELISA, ICC/IF, IHC-Fr

applications

Immunogen

Native Tissue collagen Type IV from human and bovine placenta within Cow COL4A1. Database link Q7SIB2

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

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Reactivity data

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We would suggest testing it under native conditions.", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" } } }

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Product details

Anti-Collagen IV antibody (ab6586) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IF, IHC-FoFr, IHC-Fr, IHC-FrFl,

Anti-Collagen IV antibody (ab6586) was first used in a scientific publication in 2004 and has been cited over 920 times in peer reviewed journals. It's performance in IHC and Western Blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Collagen IV antibody (ab6586) has high sensitivity and specificity.

Anti-Collagen IV antibody (ab6586) has 120 independent reviews from customers.

Anti-Collagen IV antibody (ab6586) specifically detects Collagen IV (UniProt ID: P02462; Molecular weight: ~160kDa) and is sold in 100 µg selling sizes.

Collagen IV is a key component of the extracellular matrix in tumors, contributing to tissue stiffness and promoting cancer cell invasion. It plays a significant role in tumor progression and metastasis, influencing the behaviour of cancer cells.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 and collagen type 4 plays a significant mechanical role in the structure of the basement membrane. It provides structural support and mechanical stability. Collagen IV has a molecular weight ranging approximately from 160 to 240 kDa. It is expressed in various regions including the kidney lung and vascular tissues where it contributes to the integrity and function of these organs.

Biological function summary

The primary function of collagen type IV involves forming a scaffold essential for basement membrane assembly and function. It exists as a part of a complex formation typically forming networks with other collagens and proteins. These networks are important for cellular adhesion differentiation migration and filtration processes. Collagen IV interacts with different cell surface receptors helping mediate important cellular signals.

Pathways

Collagen type 4 is integral to the extracellular matrix and integrin-mediated signaling pathways. It partners with proteins such as laminins and nidogens in these pathways contributing to cell adhesion and migration. This interaction helps regulate various cellular processes including wound healing and tissue repair. By being a part of these pathways collagen IV impacts cellular behavior and tissue homeostasis.

Dysfunction or mutations in collagen IV relate to conditions such as Alport syndrome and Goodpasture's syndrome. Alport syndrome involves mutations in collagen IV leading to kidney dysfunction and hearing loss. In Goodpasture's syndrome collagen IV becomes a target for autoimmune attack resulting in damage to the lungs and kidneys. These diseases illustrate the connection between collagen IV and other proteins like laminin which together contribute to maintaining tissue integrity and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information COL4A1

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Publications (1127)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8433 PubMed41022699

2025

Tailored collagen binding of albumin-fused hyperactive coagulation factor IX dictates in vivo distribution and functional properties.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Hovden Aaen,Maria Francesca Testa,Jeannette Nilsen,Rebecca Tarantino,Cesare Canepari,Mascia Benedusi,Sopisa Benjakul,Mari Nyquist-Andersen,Marie Leangen Herigstad,Alessio Cantore,Giuseppe Valacchi,Inger Sandlie,Francesco Bernardi,Mirko Pinotti,Alessio Branchini,Jan Terje Andersen

Neuropathology and applied neurobiology 51:e70037 PubMed40919646

2025

Intrahippocampally Injected Human Recombinant Clusterin Reduces Amyloid-β Aggregate Size in Cerebral Arteriole Walls of Clusterin Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandru Laslo,Tudor-Gabriel Boghițoiu,Laura Chinezu,Doina Manu,Adrian Dumitru Ivanescu,Bogdan Andrei Cordoș,Mark R Wilson,Claudia Banescu,John D Fryer,Ajay Verma,Jennifer M Dewing,Roxana-Octavia Carare

Frontiers in aging neuroscience 17:1634283 PubMed40904345

2025

Modulation of fenestrated vasculature in the median eminence and area postrema in response to neurotoxin exposure and its impairment in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Viana Q Pham,Melike Tutunculer,Halah Al-Dulaimi,Daniel Ardjmand,William Fleischmann,Tomas P Bachor,Allison W Xu

International journal of molecular sciences 26: PubMed40869164

2025

Study of the Effectiveness of Skin Restoration Using a Biopolymer Hydrogel Scaffold with Encapsulated Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marfa N Egorikhina,Lidia B Timofeeva,Yulia P Rubtsova,Ekaterina A Farafontova,Dariya D Linkova,Irina N Charykova,Maksim G Ryabkov,Anna A Ezhevskaya,Ekaterina A Levicheva,Diana Ya Aleynik

Cells 14: PubMed40801608

2025

Extract Supports Skin Homeostasis by Enhancing Epidermal Stem Cell Function and Reinforcing Their Extracellular Niche.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Lotti,Laetitia Cattuzzato,Xuefeng Huang,David Garandeau,Elisabetta Palazzo,Marika Quadri,Cécile Delluc,Eddy Magdeleine,Xiaojing Li,Mathilde Frechet,Alessandra Marconi

PloS one 20:e0328898 PubMed40768435

2025

Development of a tissue-specific bioscaffold for intestinal stem cell culture.

Applications

Unspecified application

Species

Unspecified reactive species

Sachin Kakar,Mathieu F Derouet,Liyue Zhang,Connor J Gillis,Frederikke Larsen,Arghya Paul,Lauren E Flynn,Samuel Asfaha

Inflammation and regeneration 45:25 PubMed40760447

2025

APJ regulates the balance between self-renewal and differentiation of vascular endothelial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man Wang,Fitriana Nur Rahmawati,Wenting Li,Zeynep Bal,Faya Nuralda Sitompul,Fumitaka Muramatsu,Weizhen Jia,Nobuyuki Takakura

Gels (Basel, Switzerland) 11: PubMed40710679

2025

Rat Islet pECM Hydrogel-Based Microencapsulation: A Protective Niche for Xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Skitel Moshe,Stasia Krishtul,Anastasia Brandis,Rotem Hayam,Shani Hamias,Mazal Faraj,Tzila Davidov,Inna Kovrigina,Limor Baruch,Marcelle Machluf

Cells 14: PubMed40710298

2025

Bioengineering a Human Dermal Equivalent Using Induced Pluripotent Stem Cell-Derived Fibroblasts to Support the Formation of a Full-Thickness Skin Construct.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Smith,David Bunton,Michael Finch,Stefan Przyborski

Nature 644:516-526 PubMed40681898

2025

Pathology-oriented multiplexing enables integrative disease mapping.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Kuehl,Yusuke Okabayashi,Milagros N Wong,Lukas Gernhold,Gabriele Gut,Nico Kaiser,Maria Schwerk,Stefanie K Gräfe,Frank Y Ma,Jovan Tanevski,Philipp S L Schäfer,Sam Mezher,Jacobo Sarabia Del Castillo,Thiago Goldbeck-Strieder,Olga Zolotareva,Michael Hartung,Fernando M Delgado Chaves,Lukas Klinkert,Ann-Christin Gnirck,Marc Spehr,David Fleck,Mehdi Joodaki,Victor Parra,Mina Shaigan,Martin Diebold,Marco Prinz,Jennifer Kranz,Johan M Kux,Fabian Braun,Oliver Kretz,Hui Wu,Florian Grahammer,Sven Heins,Marina Zimmermann,Fabian Haas,Dominik Kylies,Nicola Wanner,Jan Czogalla,Bernhard Dumoulin,Nikolay Zolotarev,Maja Lindenmeyer,Pall Karlson,Jens R Nyengaard,Marcial Sebode,Sören Weidemann,Thorsten Wiech,Hermann-Josef Groene,Nicola M Tomas,Catherine Meyer-Schwesinger,Christoph Kuppe,Rafael Kramann,Alexandre Karras,Patrick Bruneval,Pierre-Louis Tharaux,Diego Pastene,Benito Yard,Jennifer A Schaub,Phillip J McCown,Laura Pyle,Ye Ji Choi,Takashi Yokoo,Jan Baumbach,Pablo J Sáez,Ivan Costa,Jan-Eric Turner,Jeffrey B Hodgin,Julio Saez-Rodriguez,Tobias B Huber,Petter Bjornstad,Matthias Kretzler,Olivia Lenoir,David J Nikolic-Paterson,Lucas Pelkmans,Stefan Bonn,Victor G Puelles

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Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (AB6586)

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