AB6311

Anti-Collagen IV antibody [COL-94]

Name: Anti-Collagen IV antibody [COL-94] (ab6311) | Abcam
Brand: Abcam Limited
SKU: AB6311
Price: 485 USD
Availability: InStock
Rating: 4 (10 reviews)

(10 Reviews)

(51 Publications)

Anti-Collagen IV antibody [COL-94] (ab6311) is a mouse monoclonal antibody detecting Collagen IV in IHC-P. Suitable for Human.

- Over 40 publications
- Trusted since 2002

View Alternative Names

Collagen alpha-1(IV) chain, COL4A1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

IHC-P

AbReview5838****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with unpurified ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.

This image was generated using the ascites version of the product.

This image is courtesy of an abreview submitted by Birgitta Weijdegard, Sahlgrenska Cancer Center.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

Immunohistochemical analysis of paraffin-embedded human tongue tissue staining Collagen IV with ab6311 at 8μg/ml. The antibody was developed using biotin/ExtrAvidin^®-Peroxidase.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

COL-94

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human COL4A1.

P02462

Epitope

Recognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." }, "Cat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." }, "Chicken": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." }, "Goat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." }, "Primates": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "The antibody was developed using biotin/ExtrAvidin®-Peroxidase." } } }

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Product details

What is this antibody validated in?
Anti-Collagen IV antibody [COL-94] (ab6311) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Collagen IV [COL-94] (ab6311) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Collagen IV [COL-94] (ab6311) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from hybridoma cell culture

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.

Biological function summary

The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.

Pathways

Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.

Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

See full target information COL4A1

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 13:e2301142 PubMed37946678

2023

Recovery of Therapeutically Ablated Engineered Blood-Vessel Networks on a Plug-and-Play Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa A Krattiger,Lukas O Moser,Rodi Odabasi,Adolfo Odriozola,Benjamin R Simona,Valentin Djonov,Mark W Tibbitt,Martin Ehrbar

Experimental eye research 234:109605 PubMed37506755

2023

Characterization of extracellular matrix deposited by segmental trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

VijayKrishna Raghunathan,Andrews Nartey,Kamesh Dhamodaran,Hasna Baidouri,Julia A Staverosky,Kate E Keller,Keith Zientek,Ashok Reddy,Ted Acott,Janice A Vranka

Nature protocols 18:2283-2312 PubMed37165073

2023

A distal lung organoid model to study interstitial lung disease, viral infection and human lung development.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Matkovic Leko,Remy T Schneider,Tania A Thimraj,Nadine Schrode,Daniel Beitler,Hsiao-Yun Liu,Kristin Beaumont,Ya-Wen Chen,Hans-Willem Snoeck

Advanced materials (Deerfield Beach, Fla.) 35:e2209476 PubMed36724374

2023

Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Rütsche,Monica Nanni,Simon Rüdisser,Thomas Biedermann,Marcy Zenobi-Wong

APL bioengineering 7:016111 PubMed36875739

2023

Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Micek,Lauren Rosenstock,Yicheng Ma,Caitlin Hielsberg,Lauren Montemorano,Metti K Gari,Suzanne M Ponik,Pamela K Kreeger

International journal of molecular sciences 24: PubMed36835504

2023

Space Omics and Tissue Response in Astronaut Skeletal Muscle after Short and Long Duration Missions.

Applications

Unspecified application

Species

Unspecified reactive species

Dieter Blottner,Manuela Moriggi,Gabor Trautmann,Maria Hastermann,Daniele Capitanio,Enrica Torretta,Katharina Block,Joern Rittweger,Ulrich Limper,Cecilia Gelfi,Michele Salanova

Nature methods 20:149-161 PubMed36550275

2022

Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jae Song,Russ Quinn,Eric Nguyen,Christopher Hampton,Ruchi Sharma,Tea Soon Park,Céline Koster,Ty Voss,Carlos Tristan,Claire Weber,Anju Singh,Roba Dejene,Devika Bose,Yu-Chi Chen,Paige Derr,Kristy Derr,Sam Michael,Francesca Barone,Guibin Chen,Manfred Boehm,Arvydas Maminishkis,Ilyas Singec,Marc Ferrer,Kapil Bharti

Theranostics 12:3758-3775 PubMed35664054

2022

Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Liu,Xiaoxuan Ning,Lei Wei,Ying Zhou,Lijuan Zhao,Feng Ma,Ming Bai,Xiaoxia Yang,Di Wang,Shiren Sun

Cells 11: PubMed35326506

2022

The Role of CD200-CD200 Receptor in Human Blood and Lymphatic Endothelial Cells in the Regulation of Skin Tissue Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Rütsche,Katarzyna Michalak-Micka,Dominika Zielinska,Hannah Moll,Ueli Moehrlen,Thomas Biedermann,Agnes S Klar

Regenerative therapy 19:24-34 PubMed35059477

2022

Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture.

Applications

Unspecified application

Species

Unspecified reactive species

Suguru Yamasaki,Atsushi Kuwahara,Akiyoshi Kishino,Toru Kimura,Masayo Takahashi,Michiko Mandai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen IV antibody [COL-94]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (AB6311)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (51)

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