Rabbit Recombinant Multiclonal COL4A1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-frozen-sections-img424356.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251)")
![Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-frozen-sections-img424355.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424283.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424282.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424281.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (AB315251)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IHC-Fr | Flow Cyt | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.
Alternative names
Collagen alpha-1(IV) chain, COL4A1
Recommended products
Rabbit Recombinant Multiclonal COL4A1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1065
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315251 is the carrier-free version of Anti-Collagen IV antibody [RM1065] ab315250.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.
- Biological function summary
The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.
- Pathways
Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.
- Associated diseases and disorders
Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-frozen-sections-img424356.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-frozen-sections-img424355.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424283.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/1000 (0.536 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Basement membrane staining on rat kidney. The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424282.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/1000 (0.536 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the blood vessels of mouse cerebrum. The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424281.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/1000 (1.072 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Basement membrane staining on mouse kidney. The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424280.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/500 (1.072 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the blood vessels of human glioma. The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424279.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)
This data was developed using Anti-Collagen IV antibody [RM1065] ab315250, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Collagen IV with Anti-Collagen IV antibody [RM1065] ab315250 at 1/500 (1.072 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Basement membrane staining on human kidney. The section was incubated with Anti-Collagen IV antibody [RM1065] ab315250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424280.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251), expandable thumbnail](https://content.abcam.com/products/images/collagen-iv-antibody-rm1065-bsa-and-azide-free-ab315251--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424279.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [RM1065] - BSA and Azide free (ab315251)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com