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AB134800

Anti-Collagen V antibody

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(2 Publications)

Rabbit Polyclonal CO5A1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Collagen alpha-1(V) chain, COL5A1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB134800)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB134800)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin, dermal collagen labelling Collagen V with ab134800 at 5 µg/ml, after heat-induced antigen retrieval.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 8 Preservative: 0.01% Sodium azide Constituents: 4.77% Sodium borate, 0.43% Sodium chloride, 0.15% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen V also known as collagen type V is a fibrillar collagen integral to the formation and structure of the extracellular matrix. It plays a mechanical role by regulating the assembly of other fibrillar collagens such as type I. Collagen V is a heterotrimer composed of three alpha chains—two α1(V) and one α2(V)—and demonstrates a molecular weight that typically ranges between 180-200 kDa. Expression of collagen type 5 is prominent in connective tissues especially those found in skin tendon and cornea where it ensures proper collagen fibril formation and tissue stability.
Biological function summary

Collagen V coordinates with other collagen types to control fibrillogenesis the process by which collagen molecules assemble into fibrils. It exists as part of a complex within the extracellular matrix often found interacting with collagen type I influencing its diameter and the organization of fibrils. This involvement is important for maintaining the structural integrity and mechanical properties of tissues that require a well-organized fibrillar network like the sclera and the dermis.

Pathways

Collagen V participates in the regulation of the collagen biosynthesis and degradation pathway as well as the TGF-β signaling pathway. It modulates the biosynthesis pathway by ensuring a controlled assembly and deposition of collagen fibrils which is essential for tissue repair and maintenance. Through its influence on the TGF-β signaling pathway collagen type V interacts with proteins such as decorin and biglycan which are also associated with modulating collagen fibril formation and tissue morphogenesis.

Collagen V defects or imbalances can lead to conditions such as Ehlers-Danlos syndrome (EDS) and fibrosis. EDS is associated with mutations in the COL5A1 or COL5A2 genes leading to abnormal collagen V production which affects skin elasticity and joint integrity. Fibrosis involves excessive collagen deposition including collagen type V resulting in tissue stiffening and impaired function. In these disorders surrounding collagens and matrix proteins like decorin may be altered further exacerbating tissue dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
See full target information COL5A1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 7:e2131 PubMed34458452

2017

Analysis of Cancer Stromal Reaction Using an O-ring Co-culture Assay.

Applications

Unspecified application

Species

Unspecified reactive species

Vivien Jane Coulson-Thomas

Investigative ophthalmology & visual science 55:935-40 PubMed24448264

2014

Outflow tract ablation using a conditionally cytotoxic feline immunodeficiency viral vector.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Zhang,Amardeep S Dhaliwal,Harry Tseng,James D Kim,Joel S Schuman,Robert N Weinreb,Nils A Loewen
View all publications
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