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AB275890

Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free

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Rabbit Recombinant Monoclonal Collagen V antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

Collagen alpha-2(V) chain, COL5A2

3 Images
Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)
  • WB

Lab

Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)

This data was developed using ab275881, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 20625483).

Glycosylation leads to the shift of the bands.

Exposure time : 6 seconds.

All lanes:

Western blot - Anti-Collagen V antibody [EPR23762-54] (<a href='/en-us/products/primary-antibodies/collagen-v-antibody-epr23762-54-ab275881'>ab275881</a>) at 1/1000 dilution

Lane 1:

Mouse articular cartilage organ culture supernatant at 10 µL

Lane 2:

Mouse articular cartilage organ culture supernatant treated with PNGase F at 10 µL

Lane 3:

Mouse articular cartilage tissue lysate at 20 µg

Lane 4:

Mouse articular cartilage tissue lysate treated with PNGase F at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 184 kDa

Observed band size: 32 kDa,36 kDa

false

Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)
  • WB

Lab

Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)

This data was developed using ab275881, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 23262228, 20625483).

Exposure time : Lane 1 : 92 seconds Lane 2 : 1 seconds.

All lanes:

Western blot - Anti-Collagen V antibody [EPR23762-54] (<a href='/en-us/products/primary-antibodies/collagen-v-antibody-epr23762-54-ab275881'>ab275881</a>) at 1/1000 dilution

Lane 1:

Rat placenta organ culture supernatant at 30 µL

Lane 2:

Rat articular cartilage tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 184 kDa

Observed band size: 145 kDa,32 kDa

false

Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)
  • WB

Lab

Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (AB275890)

This data was developed using ab275881, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 20625483).

Low expression : Mouse PBMCs (database : HPA).

Exposure time : Lane 1 : 5 seconds Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-Collagen V antibody [EPR23762-54] (<a href='/en-us/products/primary-antibodies/collagen-v-antibody-epr23762-54-ab275881'>ab275881</a>) at 1/1000 dilution

Lane 1:

Mouse articular cartilage tissue lysate at 20 µg

Lane 2:

Mouse PBMCs (peripheral blood mononuclear cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 184 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23762-54

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen of this antibody is based on Collagen V-COL5A2, low homology with Collagen V-COL5A1 based on immunogen design.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab275890 is the carrier-free version of ab275881.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen V also known as collagen type V is a fibrillar collagen integral to the formation and structure of the extracellular matrix. It plays a mechanical role by regulating the assembly of other fibrillar collagens such as type I. Collagen V is a heterotrimer composed of three alpha chains—two α1(V) and one α2(V)—and demonstrates a molecular weight that typically ranges between 180-200 kDa. Expression of collagen type 5 is prominent in connective tissues especially those found in skin tendon and cornea where it ensures proper collagen fibril formation and tissue stability.
Biological function summary

Collagen V coordinates with other collagen types to control fibrillogenesis the process by which collagen molecules assemble into fibrils. It exists as part of a complex within the extracellular matrix often found interacting with collagen type I influencing its diameter and the organization of fibrils. This involvement is important for maintaining the structural integrity and mechanical properties of tissues that require a well-organized fibrillar network like the sclera and the dermis.

Pathways

Collagen V participates in the regulation of the collagen biosynthesis and degradation pathway as well as the TGF-β signaling pathway. It modulates the biosynthesis pathway by ensuring a controlled assembly and deposition of collagen fibrils which is essential for tissue repair and maintenance. Through its influence on the TGF-β signaling pathway collagen type V interacts with proteins such as decorin and biglycan which are also associated with modulating collagen fibril formation and tissue morphogenesis.

Collagen V defects or imbalances can lead to conditions such as Ehlers-Danlos syndrome (EDS) and fibrosis. EDS is associated with mutations in the COL5A1 or COL5A2 genes leading to abnormal collagen V production which affects skin elasticity and joint integrity. Fibrosis involves excessive collagen deposition including collagen type V resulting in tissue stiffening and impaired function. In these disorders surrounding collagens and matrix proteins like decorin may be altered further exacerbating tissue dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices (By similarity).
See full target information Collagen alpha-2(V) chain
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com