AB180855

Anti-Collagen VI alpha 2 antibody [EPR7889(N)]

Name: Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855) | Abcam
Brand: Abcam Limited
SKU: AB180855
Price: 350 USD
Availability: InStock
Rating: 4 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal CO6A2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Collagen alpha-2(VI) chain, COL6A2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (AB180855)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (AB180855)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Collagen VI alpha 2 with Purified ab180855 at 1 : 4000 dilution (0.413 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Unknown

Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (AB180855)

All lanes:

Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855) at 1/1000 dilution

All lanes:

Human skin lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 109 kDa

Observed band size: 140 kDa

false

Carrier free

Anti-Collagen VI alpha 2 antibody [EPR7889(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7889(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "For unpurified form, use 1/500 - 1/1000 dilution", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VI alpha 2 also known as COL6A2 or anti-collagen VI is a protein that is an essential component of the extracellular matrix. It forms a part of the collagen VI complex which is important for connective tissue stability and function. COL6A2 has a molecular mass of approximately 130 kDa. It is widely expressed in various tissues including muscle skin and the basement membrane of blood vessels indicating its diverse functional role.

Biological function summary

The COL6A2 protein contributes to maintaining tissue integrity and resilience. It forms a beaded filament structure as part of a collagen VI tetramer when combined with other alpha chains (alpha 1 and alpha 3). These interactions at the molecular level help to provide tensile strength and structural support to many tissues especially in muscle where it impacts elasticity and repair mechanisms.

Pathways

COL6A2 and its associated complex participate in the extracellular matrix organization and cell adhesion pathways. In these processes it interacts with proteins such as integrins and laminins which are important for cellular attachment and communication. Such pathways underline the significant roles COL6A2 plays in maintaining normal tissue architecture and cellular environments.

The COL6A2 protein has strong associations with conditions like myosclerosis and Bethlem myopathy. Mutations or dysfunctions in the COL6A2 gene can disrupt the extracellular matrix leading to musculoskeletal disorders. It also shows interactions with other proteins like dystrophin in these pathological states emphasizing its involvement in muscular integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Collagen VI acts as a cell-binding protein.

See full target information Collagen alpha-2(VI) chain

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:660 PubMed40528195

2025

Interleukin-1β mediates a tumor-supporting environment prompted by IGF1 in triple-negative breast cancer (TNBC).

Applications

Unspecified application

Species

Unspecified reactive species

Domenica Scordamaglia,Marianna Talia,Francesca Cirillo,Azzurra Zicarelli,Adelina Assunta Mondino,Salvatore De Rosis,Marika Di Dio,Francesca Silvestri,Chiara Meliti,Anna Maria Miglietta,Carlo Capalbo,Ernestina Marianna De Francesco,Antonino Belfiore,Marcello Maggiolini,Rosamaria Lappano

American journal of physiology. Lung cellular and molecular physiology 324:L799-L814 PubMed37039368

2023

Age-associated differences in the human lung extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Maunick Lefin Koloko Ngassie,Maaike De Vries,Theo Borghuis,Wim Timens,Don D Sin,David Nickle,Philippe Joubert,Peter Horvatovich,György Marko-Varga,Jacob J Teske,Judith M Vonk,Reinoud Gosens,Y S Prakash,Janette K Burgess,Corry-Anke Brandsma

OncoTargets and therapy 13:4999-5016 PubMed32581558

2020

Comprehensive Transcriptomic Analysis and Experimental Validation Identify lncRNA HOXA-AS2/miR-184/COL6A2 as the Critical ceRNA Regulation Involved in Low-Grade Glioma Recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Yu Chen,Xiao-Dong Li,Wei-Ning Ma,Han Li,Miao-Miao Li,Xin-Yu Yang,Shao-Yi Li

Oncoscience 4:178-188 PubMed29344556

2017

Extracellular matrix composition modulates angiosarcoma cell attachment and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Noel L Shaheen,Esha Kataria,Jocelyn Antony,Dana Galvan,Yessenia Ballou,Brad A Bryan

BMC research notes 10:358 PubMed28755659

2017

Authentication of collagen VI antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie Endicott,Paul Holden,Jamie Fitzgerald

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen VI alpha 2 antibody [EPR7889(N)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (AB180855)

Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (AB180855)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise