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AB6588

Anti-Collagen VI antibody

5

(21 Reviews)

|

(151 Publications)

Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody detecting Collagen VI in Western Blot. Suitable for Human.

- Over 150 publications
- Trusted since 2002

View Alternative Names

Collagen alpha-1(VI) chain, COL6A1

1 Images
Western blot - Anti-Collagen VI antibody (AB6588)
  • WB

Unknown

Western blot - Anti-Collagen VI antibody (AB6588)

All lanes:

Western blot - Anti-Collagen VI antibody (ab6588) at 1/5000 dilution

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 109 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Human COL6A1.

P12109

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Reactivity data

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Product details

What is this antibody validated in?
Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Collagen VI?
Anti-Collagen VI (ab6588) specifically detects a band for Collagen VI (UniProt: P12109) at a molecular weight of 108kDa.

Trusted by the scientific community
Anti-Collagen VI (ab6588) was first used in a scientific publication in 2002 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Collagen VI (ab6588) has over 20 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.
Biological function summary

Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.

Pathways

Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.

Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Collagen VI acts as a cell-binding protein.
See full target information COL6A1

Publications (151)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 16: PubMed38050706

2023

Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique O Riddell,John C W Hildyard,Rachel C M Harron,Frances Taylor-Brown,Joe N Kornegay,Dominic J Wells,Richard J Piercy

Interface focus 13:20230047 PubMed38106917

2023

Pirfenidone increases transverse tubule length in the infarcted rat myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Hussam Moammer,Jizhong Bai,Timothy L M Jones,Marie Ward,Caroyln Barrett,David J Crossman

International journal of molecular sciences 24: PubMed37685934

2023

Effects of Aging on Collagen in the Skeletal Muscle of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kanazawa,Ryo Miyachi,Takashi Higuchi,Hiaki Sato

Scientific reports 12:22474 PubMed36577792

2022

Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xing,Yaqing Huang,Britta H Kunkemoeller,Peter J Dahl,Ohvia Muraleetharan,Nikhil S Malvankar,Michael P Murrell,Themis R Kyriakides

Pathophysiology : the official journal of the International Society for Pathophysiology 29:640-649 PubMed36548206

2022

The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Babu Raja Maharjan,Susan V McLennan,Stephen M Twigg,Paul F Williams

Translational vision science & technology 11:10 PubMed36374486

2022

Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.

Applications

Unspecified application

Species

Unspecified reactive species

John P M Wood,Glyn Chidlow,Luke A Halliday,Robert J Casson,Dinesh Selva,Michelle Sun

Human mutation 43:1945-1955 PubMed35998264

2022

A loss-of-function cysteine mutant in fibulin-3 (EFEMP1) forms aberrant extracellular disulfide-linked homodimers and alters extracellular matrix composition.

Applications

Unspecified application

Species

Unspecified reactive species

DaNae R Woodard,Steffi Daniel,Emi Nakahara,Ali Abbas,Sophia M DiCesare,Gracen E Collier,John D Hulleman

International journal of molecular sciences 23: PubMed35805907

2022

In Vitro and Ectopic In Vivo Studies toward the Utilization of Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gyözö Lehoczky,Raluca Elena Trofin,Queralt Vallmajo-Martin,Shikha Chawla,Karoliina Pelttari,Marcus Mumme,Martin Haug,Christian Egloff,Marcel Jakob,Martin Ehrbar,Ivan Martin,Andrea Barbero

Biomolecules 12: PubMed35740962

2022

Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models.

Applications

Unspecified application

Species

Unspecified reactive species

Atiya M Sarmin,Nadia El Moussaid,Ratima Suntornnond,Eleanor J Tyler,Yang-Hee Kim,Stefania Di Cio,William V Megone,Oliver Pearce,Julien E Gautrot,Jonathan Dawson,John T Connelly

Cells 11: PubMed35563866

2022

Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Rampin,Ioannis Skoufos,Michael Raghunath,Athina Tzora,Nikolaos Diakakis,Nikitas Prassinos,Dimitrios I Zeugolis
View all publications

Product promise

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