AB6588

Anti-Collagen VI antibody

Name: Anti-Collagen VI antibody (ab6588) | Abcam
Brand: Abcam Limited
SKU: AB6588
Price: 505 USD
Availability: InStock
Rating: 5 (21 reviews)

(21 Reviews)

(151 Publications)

Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody detecting Collagen VI in Western Blot. Suitable for Human.

- Over 150 publications
- Trusted since 2002

View Alternative Names

Collagen alpha-1(VI) chain, COL6A1

1 Images

Unknown

Western blot - Anti-Collagen VI antibody (AB6588)

All lanes:

Western blot - Anti-Collagen VI antibody (ab6588) at 1/5000 dilution

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 109 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human COL6A1.

P12109

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Collagen VI?
Anti-Collagen VI (ab6588) specifically detects a band for Collagen VI (UniProt: P12109) at a molecular weight of 108kDa.

Trusted by the scientific community
Anti-Collagen VI (ab6588) was first used in a scientific publication in 2002 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Collagen VI (ab6588) has over 20 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.

Biological function summary

Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.

Pathways

Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.

Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Collagen VI acts as a cell-binding protein.

See full target information COL6A1

style

left-column

Publications (151)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 16: PubMed38050706

2023

Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique O Riddell,John C W Hildyard,Rachel C M Harron,Frances Taylor-Brown,Joe N Kornegay,Dominic J Wells,Richard J Piercy

Interface focus 13:20230047 PubMed38106917

2023

Pirfenidone increases transverse tubule length in the infarcted rat myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Hussam Moammer,Jizhong Bai,Timothy L M Jones,Marie Ward,Caroyln Barrett,David J Crossman

International journal of molecular sciences 24: PubMed37685934

2023

Effects of Aging on Collagen in the Skeletal Muscle of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kanazawa,Ryo Miyachi,Takashi Higuchi,Hiaki Sato

Scientific reports 12:22474 PubMed36577792

2022

Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xing,Yaqing Huang,Britta H Kunkemoeller,Peter J Dahl,Ohvia Muraleetharan,Nikhil S Malvankar,Michael P Murrell,Themis R Kyriakides

Pathophysiology : the official journal of the International Society for Pathophysiology 29:640-649 PubMed36548206

2022

The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Babu Raja Maharjan,Susan V McLennan,Stephen M Twigg,Paul F Williams

Translational vision science & technology 11:10 PubMed36374486

2022

Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.

Applications

Unspecified application

Species

Unspecified reactive species

John P M Wood,Glyn Chidlow,Luke A Halliday,Robert J Casson,Dinesh Selva,Michelle Sun

Human mutation 43:1945-1955 PubMed35998264

2022

A loss-of-function cysteine mutant in fibulin-3 (EFEMP1) forms aberrant extracellular disulfide-linked homodimers and alters extracellular matrix composition.

Applications

Unspecified application

Species

Unspecified reactive species

DaNae R Woodard,Steffi Daniel,Emi Nakahara,Ali Abbas,Sophia M DiCesare,Gracen E Collier,John D Hulleman

International journal of molecular sciences 23: PubMed35805907

2022

In Vitro and Ectopic In Vivo Studies toward the Utilization of Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gyözö Lehoczky,Raluca Elena Trofin,Queralt Vallmajo-Martin,Shikha Chawla,Karoliina Pelttari,Marcus Mumme,Martin Haug,Christian Egloff,Marcel Jakob,Martin Ehrbar,Ivan Martin,Andrea Barbero

Biomolecules 12: PubMed35740962

2022

Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models.

Applications

Unspecified application

Species

Unspecified reactive species

Atiya M Sarmin,Nadia El Moussaid,Ratima Suntornnond,Eleanor J Tyler,Yang-Hee Kim,Stefania Di Cio,William V Megone,Oliver Pearce,Julien E Gautrot,Jonathan Dawson,John T Connelly

Cells 11: PubMed35563866

2022

Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Rampin,Ioannis Skoufos,Michael Raghunath,Athina Tzora,Nikolaos Diakakis,Nikitas Prassinos,Dimitrios I Zeugolis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen VI antibody

Western blot - Anti-Collagen VI antibody (AB6588)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (151)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise