AB6588

Anti-Collagen VI antibody

Name: Anti-Collagen VI antibody (ab6588) | Abcam
Brand: Abcam Limited
SKU: AB6588
Price: 505 USD
Availability: InStock
Rating: 5 (21 reviews)

(21 Reviews)

(183 Publications)

Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody detecting Collagen VI in Western Blot. Suitable for Human.

- Over 150 publications
- Trusted since 2002

View Alternative Names

Collagen alpha-1(VI) chain, COL6A1

1 Images

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Western blot - Anti-Collagen VI antibody (AB6588)

All lanes:

Western blot - Anti-Collagen VI antibody (ab6588) at 1/5000 dilution

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 109 kDa

Observed band size: 100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human COL6A1.

P12109

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Collagen VI antibody (ab6588) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Collagen VI?
Anti-Collagen VI (ab6588) specifically detects a band for Collagen VI (UniProt: P12109) at a molecular weight of 108kDa.

Trusted by the scientific community
Anti-Collagen VI (ab6588) was first used in a scientific publication in 2002 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Collagen VI (ab6588) has over 20 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.

Biological function summary

Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.

Pathways

Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.

Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Collagen VI acts as a cell-binding protein.

See full target information COL6A1

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Publications (183)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed41034152

2025

Combined ADAMTS10 and ADAMTS17 inactivation exacerbates bone shortening and skin phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Nandaraj Taye,Stylianos Z Karoulias,Zerina Balic,Lauren W Wang,Belinda B Willard,Daniel Martin,Daniel Richard,Alexander S Okamoto,Terence D Capellini,Suneel S Apte,Dirk Hubmacher

eLife 12: PubMed41025959

2025

Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander N Kapustin,Sofia Serena Tsakali,Meredith Whitehead,George Chennell,Meng-Ying Wu,Chris Molenaar,Anton Kutikhin,Yimeng Chen,Sadia Ahmad,Leo Bogdanov,Maxim Sinitsky,Kseniya Rubina,Aled Clayton,Frederik J Verweij,Dirk Michiel Pegtel,Simona Zingaro,Arseniy Lobov,Bozhana Zainullina,Dylan Owen,Maddy Parsons,Richard E Cheney,Derek T Warren,Martin James Humphries,Thomas Iskratsch,Mark Holt,Catherine M Shanahan

Nature 646:934-944 PubMed40903576

2025

Dynamic fibroblast-immune interactions shape recovery after brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan A Ewing-Crystal,Nicholas M Mroz,Amara Larpthaveesarp,Carlos O Lizama,Remy Pennington,Pailin Chiaranunt,Jason I Dennis,Anthony A Chang,Eric Dean Merrill,Sofia E Caryotakis,Nikhita Kirthivasan,Leon Teo,Tatsuya Tsukui,Aditya Katewa,Gabriel L McKinsey,Sophia C K Nelson,Agnieszka Ciesielska,Nicole C Lummis,Lucija Pintarić,Madelene W Dahlgren,Amha Atakilit,Helena Paidassi,Saket Jain,Xiaodan Liu,Duan Xu,Manish K Aghi,James A Bourne,Jeanne T Paz,Richard Daneman,Fernando F Gonzalez,Dean Sheppard,Anna V Molofsky,Thomas D Arnold,Ari B Molofsky

PLoS genetics 21:e1011806 PubMed40825068

2025

The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Soomin Cho,Emilia Servián-Morilla,Victoria Navarro,Beatriz Rodriguez-Gonzalez,Youxi Yuan,Raquel Cano,Arjun A Rambhiya,Radbod Darabi,Robert S Haltiwanger,Carmen Paradas,Hamed Jafar-Nejad

International journal of molecular sciences 26: PubMed40649868

2025

Furosemide Promotes Inflammatory Activation and Myocardial Fibrosis in Swine with Tachycardia-Induced Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Nisha Plavelil,Robert Goldstein,Michael G Klein,Luke Michaelson,Mark C Haigney,Maureen N Hood

Neuropathology and applied neurobiology 51:e70020 PubMed40400418

2025

Collablots: Quantification of Collagen VI Levels and Its Structural Disorganisation in Cell Cultures From Patients With Collagen VI-Related Dystrophies.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Osegui-Barcenilla,Maria Sendino,Sergio Martín-González,Itziar González-Moro,Ainhoa Benito-Agustino,Noemi Torres-Conde,Andrea López-Martínez,Cecilia Jiménez-Mallebrera,Arístides López-Márquez,Virginia Arechavala-Gomeza

The Journal of clinical investigation 135: PubMed40309777

2025

Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Payam Mohassel,Hailey Hearn,Jachinta Rooney,Yaqun Zou,Kory Johnson,Gina Norato,Matthew A Nalls,Pomi Yun,Tracy Ogata,Sarah Silverstein,David A Sleboda,Thomas J Roberts,Daniel B Rifkin,Carsten G Bönnemann

Neuropathology and applied neurobiology 51:e70014 PubMed40164574

2025

Computational Analysis of SOD1-G93A Mouse Muscle Biomarkers for Comprehensive Assessment of ALS Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Gómez-Gálvez,Victoria Navarro,Ana M Castro,Carmen Paradas,Luis M Escudero

Cartilage :19476035241313179 PubMed39925233

2025

Cartilage Organoids from Articular Chondroprogenitor Cells and Their Potential to Produce Neo-Hyaline Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Daphne M A Menssen,Jeske C A Feenstra,Rob P A Janssen,Florencia Abinzano,Keita Ito

Frontiers in cardiovascular medicine 11:1430772 PubMed39691494

2024

Protective effect of UDCA against IL-11- induced cardiac fibrosis is mediated by TGR5 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

B Reilly-O'Donnell,E Ferraro,R Tikhomirov,R Nunez-Toldra,A Shchendrygina,L Patel,Y Wu,A L Mitchell,A Endo,L Adorini,R A Chowdhury,P K Srivastava,F S Ng,C Terracciano,C Williamson,J Gorelik

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen VI antibody

Western blot - Anti-Collagen VI antibody (AB6588)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (183)

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