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Rabbit Polyclonal COL6A1 antibody. Suitable for WB and reacts with Human samples. Cited in 151 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL6A1.


Images

Western blot - Anti-Collagen VI antibody (AB6588), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Human COL6A1. Database link P12109

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Dog
Predicted
Mammals
Predicted
Pig
Predicted
Rabbit
Predicted
Sheep
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Predicted
Predicted

Species

Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Collagen VI acts as a cell-binding protein.

Alternative names

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Rabbit Polyclonal COL6A1 antibody. Suitable for WB and reacts with Human samples. Cited in 151 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL6A1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Native Full Length Protein corresponding to Human COL6A1. Database link P12109
Purification technique

Affinity purification Immunogen

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Concentration
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Purification notes

Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.

Biological function summary

Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.

Pathways

Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.

Associated diseases and disorders

Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.

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1 product image

  • Western blot - Anti-Collagen VI antibody (ab6588), expandable thumbnail

    Western blot - Anti-Collagen VI antibody (ab6588)

    All lanes: Western blot - Anti-Collagen VI antibody (ab6588) at 1/5000 dilution

    All lanes: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 109 kDa

    Observed band size: 100 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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