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Rabbit Recombinant Monoclonal COL6A1 antibody. Carrier free. Suitable for WB, mIHC, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (AB271938), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (AB271938), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (AB271938), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (AB271938), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (AB271938), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBmIHCIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Collagen VI acts as a cell-binding protein.

Alternative names

Collagen alpha-1(VI) chain, COL6A1

Recommended products

Rabbit Recombinant Monoclonal COL6A1 antibody. Carrier free. Suitable for WB, mIHC, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples.

Alternative names

Collagen alpha-1(VI) chain, COL6A1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR17072

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab271938 is the carrier-free version of Anti-Collagen VI antibody [EPR17072] ab182744.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.

Biological function summary

Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.

Pathways

Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.

Associated diseases and disorders

Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Collagen VI with Anti-Collagen VI antibody [EPR17072] ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around sinusoidal endothelial basement membranes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue.

    Panel A:  Merged staining of Collagen VI (Anti-Collagen VI antibody [EPR17072] ab182744; green), anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363; red) and anti-Lamin B1 (Anti-Lamin B1 antibody [EPR22165-121] ab229025; magenta).

    Panel B: Anti-Collagen VI (green) stained on extracellular matrix.

    Panel C: Anti-CD68 (red) stained on Kupffer cells.

    Panel D: Anti-Lamin B1 (magenta) stained on nuclear envelope.

    Key protocol steps:  The section was incubated in three rounds of staining with Anti-Collagen VI antibody [EPR17072] ab182744 (1/1000 dilution), Anti-CD68 antibody [EPR20545] ab213363 (1/1000 dilution) and Anti-Lamin B1 antibody [EPR22165-121] ab229025 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    DAPI was used as a nuclear counter stain. A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Collagen VI with Anti-Collagen VI antibody [EPR17072] ab182744 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-

    -ve control1 - Anti-Collagen VI antibody [EPR17072] ab182744 at 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2 - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Collagen VI with Anti-Collagen VI antibody [EPR17072] ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining on Human cardiac sarcolemma and interstitium is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Collagen VI with Anti-Collagen VI antibody [EPR17072] ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around basement membranes of Mouse renal tubules is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Collagen VI with Anti-Collagen VI antibody [EPR17072] ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around Rat gastric epithelial basement membranes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Collagen VI (ab271938, magenta; Opal™690), anti-Cytokeratin 5 (Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free ab236216, green; Opal™520) and anti-Prostate Specific Antigen (Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-Collagen VI stained on stroma. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab271938 (1/500), Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free ab236216 (1/400), and Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen VI antibody [EPR17072] ab182744).

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast labelling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [SP69] ab93741 at 1/200 dilution (1.02 µg/mL) (B), Collagen VI with ab271938 at 1/500 dilution (2.084 μg/ml) (C) and Cytokeratin 14 with Anti-Cytokeratin 14 antibody [SP53] - BSA and Azide free ab236439 at 1/2000 dilution ( 0.519 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Panel A: merged staining of anti-Cytokeratin 14 (magenta; Opal™690), anti-Cytokeratin 18 (green; Opal™520) and anti-Collagen VI (red; Opal™570) on human breast.
    Panel B: anti-Cytokeratin 18 stained on luminal epithelial cells.
    Panel C: anti-Collagen VI stained on stroma.
    Panel D: anti-Cytokeratin 14 stained on myoepithelial cells.

    The section was incubated in three rounds of staining: in the order of Anti-Cytokeratin 14 antibody [SP53] - BSA and Azide free ab236439 for 30 mins, Anti-Cytokeratin 18 antibody [SP69] ab93741 for 10 mins, and ab271938 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] - BSA and Azide free (ab271938)

    Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (Anti-PCSK2 antibody [EPR23578-19] ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (Anti-CYP11A1 antibody [EPR24868-86] ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Collagen VI stained on extracellular matrix (Anti-Collagen VI antibody [EPR17072] ab182744; red; Opal™570) at 1:1000 (1.518 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of Anti-PCSK2 antibody [EPR23578-19] ab274418, Anti-CYP11A1 antibody [EPR24868-86] ab272494, and Anti-Collagen VI antibody [EPR17072] ab182744 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    This data was developed using Anti-Collagen VI antibody [EPR17072] ab182744, the same antibody clone in a different buffer formulation.

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