Anti-Collagen VI antibody [EPR17077] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - BSA and Azide free (AB240350)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on stromal cells of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199720).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - BSA and Azide free (AB240350)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Extracellular matrix staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199720).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Collagen VI antibody [EPR17077] - BSA and Azide free (AB240350)

Lanes 1 - 3 : Merged signal (red and green). Green - ab199720 observed at 109 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab199720 was shown to recognize Collagen VI in wild-type Hek 293T cells as signal was lost at the expected MW in COL6A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and COL6A1 knockout samples were subjected to SDS-PAGE. ab199720 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199720).

All lanes:

Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (<a href='/en-us/products/primary-antibodies/collagen-vi-antibody-epr17077-c-terminal-ab199720'>ab199720</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T whole cell lysate at 20 µg

Lane 2:

COL6A1 knockout HEK-293T whole cell lysate at 20 µg

Lane 3:

Human Skeletal Muscle whole cell lysate at 20 µg

Predicted band size: 109 kDa

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