Rabbit Recombinant Monoclonal COL6A1 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img2824.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720)")
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img83761.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720)")
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img146726.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108141.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (AB199720)")
Publications
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- Science advances 6:2020Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew L Wishart et. al.PubMed 33087348
- The American journal of pathology 189:1423-14342019High-Temperature Requirement A1 Protease as a Rate-Limiting Factor in the Development of Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter H Chen et. al.PubMed 31051168
- Archives of oral biology 98:17-252018Insulin-like growth factor-1 engaged in the mandibular condylar cartilage degeneration induced by experimental unilateral anterior crossbite.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongmei Wang,Hongxu Yang,Mian Zhang,Hongyun Zhang,Lei Lu,Jing Zhang,Xianghong Wan,Meiqing WangPubMed 30419485
- BMC research notes 10:3582017Authentication of collagen VI antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJamie Endicott et. al.PubMed 28755659
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Alternative names
Collagen alpha-1(VI) chain, COL6A1
Recommended products
Rabbit Recombinant Monoclonal COL6A1 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR17077
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.
- Biological function summary
Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.
- Pathways
Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.
- Associated diseases and disorders
Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img2824.jpg "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Lanes 1 - 3: Merged signal (red and green). Green - ab199720 observed at 109 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.ab199720 was shown to recognize Collagen VI in wild-type Hek 293T cells as signal was lost at the expected MW in COL6A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and COL6A1 knockout samples were subjected to SDS-PAGE. ab199720 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/1000 dilution
Lane 1: Wild-type HEK-293T whole cell lysate at 20 µg
Lane 2: COL6A1 knockout HEK-293T whole cell lysate at 20 µg
Lane 3: Human Skeletal Muscle whole cell lysate at 20 µg
Predicted band size: 109 kDa
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img83761.jpg "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
All lanes: Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/10000 dilution
Lane 1: Human fetal heart at 20 µg
Lane 2: Human skeletal muscle at 20 µg
Lane 3: WI-38 (Human lung) whole cell lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 147 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114698.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on stromal cells of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--western-blot-img146726.jpg "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
All lanes: Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/1000 dilution
Lane 1: Mouse heart at 10 µg
Lane 2: Mouse kidney at 10 µg
Lane 3: Mouse spleen at 10 µg
Lane 4: Rat heart at 10 µg
Lane 5: Rat kidney at 10 µg
Lane 6: Rat spleen at 10 µg
Lane 7: NIH/3T3 (mouse embryo) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 147 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108141.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Extracellular matrix staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434752.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human palatine tonsil tissue. Stained with ab199720 at 1/500 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720), expandable thumbnail](https://content.abcam.com/products/images/collagen-vi-antibody-epr17077-c-terminal-ab199720--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434752.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
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