AB223639

Anti-Collagen VII antibody

Name: Anti-Collagen VII antibody (ab223639) | Abcam
Brand: Abcam Limited
SKU: AB223639
Price: 525 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal Collagen VII antibody. Suitable for WB and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100.

View Alternative Names

Collagen alpha-1(VII) chain, Long-chain collagen, LC collagen, COL7A1

1 Images

Supplier Data

Western blot - Anti-Collagen VII antibody (AB223639)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Collagen VII antibody (ab223639) at 0.5 µg/mL

All lanes:

Rat testis lysate (in RIPA buffer) at 35 µg

Predicted band size: 295 kDa

Observed band size: 250 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q02388

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab223639 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VII also known as collagen type VII or collagen 7 is an important structural component of the extracellular matrix. It measures approximately 290 kDa in mass. Researchers identified it chiefly in stratified squamous epithelia and the basement membrane zone of the skin. Collagen VII plays a mechanical role as anchoring fibrils maintaining skin integrity by attaching the dermal tissue to the epidermal basement membrane. In marmoset dermal tissue similar functions are observed indicating a conserved role in primates.

Biological function summary

Collagen VII forms part of a larger complex known as anchoring fibrils. These fibrils play an essential role in providing structural support and maintaining mechanical stability of tissues. The protein enhances the connection between the epidermis and dermis preventing separation between these layers. Its presence signifies the maintenance of tissue integrity and resilience critical for normal skin function.

Pathways

Collagen type VII interacts within the epidermal basement membrane zone part of the broader extracellular matrix (ECM) remodeling pathway. It collaborates with other ECM components like collagen type I and collagen type III which all contribute to skin strength and elasticity. The interplay between these collagens ensures the continuous renewal and repair of skin tissues essential for wound healing and overall skin health.

Collagen VII mutations or deficiencies lead to dystrophic epidermolysis bullosa (DEB) a genetic skin condition characterized by severe blistering. This disorder arises from defective anchoring fibril formation causing skin layers to separate easily. The interplay between collagen VII and other collagens like collagen type I becomes compromised exacerbating tissue fragility. Understanding these interactions provides insight into targeted treatments and management strategies for DEB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

See full target information Collagen alpha-1(VII) chain

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:1756-1768 PubMed35198071

2022

A light-driven dual-nanotransformer with deep tumor penetration for efficient chemo-immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Peng,Fangman Chen,Yulu Liu,Fan Zhang,Lei Cao,Qiannan You,Dian Yang,Zhimin Chang,Mingfeng Ge,Li Li,Zheng Wang,Qian Mei,Dan Shao,Meiwan Chen,Wen-Fei Dong

Molecular therapy. Nucleic acids 26:1336-1350 PubMed34853731

2021

Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1.

Applications

Unspecified application

Species

Unspecified reactive species

Yankang Cui,Chenkui Miao,Shouyong Liu,Jingyuan Tang,Jing Zhang,Hengtao Bu,Yuhao Wang,Chao Liang,Meiling Bao,Chao Hou,Jiajin Wu,Xiaochao Chen,Xiang Zhang,Zengjun Wang,Bianjiang Liu

Scientific reports 10:16717 PubMed33028893

2020

Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea.

Applications

Unspecified application

Species

Unspecified reactive species

Paramananda Saikia,Jack S Crabb,Luciana L Dibbin,Madison J Juszczak,Belinda Willard,Geeng-Fu Jang,Thomas Michael Shiju,John W Crabb,Steven E Wilson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen VII antibody

Western blot - Anti-Collagen VII antibody (AB223639)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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