Goat Polyclonal Collagen VII antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100.
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- Molecular therapy. Nucleic acids 26:1336-13502021Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYankang Cui,Chenkui Miao,Shouyong Liu,Jingyuan Tang,Jing Zhang,Hengtao Bu,Yuhao Wang,Chao Liang,Meiling Bao,Chao Hou,Jiajin Wu,Xiaochao Chen,Xiang Zhang,Zengjun Wang,Bianjiang LiuPubMed 34853731
- Scientific reports 10:167172020Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParamananda Saikia et. al.PubMed 33028893
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q02388
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Dog | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-1.50000 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Pig, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Alternative names
Collagen alpha-1(VII) chain, Long-chain collagen, LC collagen, COL7A1
Recommended products
Goat Polyclonal Collagen VII antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Collagen alpha-1(VII) chain aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q02388
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab223639 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen VII also known as collagen type VII or collagen 7 is an important structural component of the extracellular matrix. It measures approximately 290 kDa in mass. Researchers identified it chiefly in stratified squamous epithelia and the basement membrane zone of the skin. Collagen VII plays a mechanical role as anchoring fibrils maintaining skin integrity by attaching the dermal tissue to the epidermal basement membrane. In marmoset dermal tissue similar functions are observed indicating a conserved role in primates.
- Biological function summary
Collagen VII forms part of a larger complex known as anchoring fibrils. These fibrils play an essential role in providing structural support and maintaining mechanical stability of tissues. The protein enhances the connection between the epidermis and dermis preventing separation between these layers. Its presence signifies the maintenance of tissue integrity and resilience critical for normal skin function.
- Pathways
Collagen type VII interacts within the epidermal basement membrane zone part of the broader extracellular matrix (ECM) remodeling pathway. It collaborates with other ECM components like collagen type I and collagen type III which all contribute to skin strength and elasticity. The interplay between these collagens ensures the continuous renewal and repair of skin tissues essential for wound healing and overall skin health.
- Associated diseases and disorders
Collagen VII mutations or deficiencies lead to dystrophic epidermolysis bullosa (DEB) a genetic skin condition characterized by severe blistering. This disorder arises from defective anchoring fibril formation causing skin layers to separate easily. The interplay between collagen VII and other collagens like collagen type I becomes compromised exacerbating tissue fragility. Understanding these interactions provides insight into targeted treatments and management strategies for DEB.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Collagen VII antibody (ab223639)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-Collagen VII antibody (ab223639) at 0.5 µg/mL
All lanes: Rat testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 295 kDa
Observed band size: 250 kDa
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