AB93350

Anti-Collagen VII antibody - BSA and Azide free

(2 Reviews)

(17 Publications)

Rabbit Polyclonal Collagen VII antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Common marmoset samples. Cited in 17 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human Collagen alpha-1(VII) chain.

View Alternative Names

Collagen alpha-1(VII) chain, Long-chain collagen, LC collagen, COL7A1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Collagen VII antibody - BSA and Azide free (AB93350)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen VII antibody - BSA and Azide free (AB93350)

Immunofluorescence staining of Collagen VII in normal human keratinocytes. Second antibody for staining of cells : goat-anti-rabbit IgG Alexa 488

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Common marmoset

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human Collagen alpha-1(VII) chain.

Q02388

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2.5-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Common marmoset": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2.5-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VII also known as collagen type VII or collagen 7 is an important structural component of the extracellular matrix. It measures approximately 290 kDa in mass. Researchers identified it chiefly in stratified squamous epithelia and the basement membrane zone of the skin. Collagen VII plays a mechanical role as anchoring fibrils maintaining skin integrity by attaching the dermal tissue to the epidermal basement membrane. In marmoset dermal tissue similar functions are observed indicating a conserved role in primates.

Biological function summary

Collagen VII forms part of a larger complex known as anchoring fibrils. These fibrils play an essential role in providing structural support and maintaining mechanical stability of tissues. The protein enhances the connection between the epidermis and dermis preventing separation between these layers. Its presence signifies the maintenance of tissue integrity and resilience critical for normal skin function.

Pathways

Collagen type VII interacts within the epidermal basement membrane zone part of the broader extracellular matrix (ECM) remodeling pathway. It collaborates with other ECM components like collagen type I and collagen type III which all contribute to skin strength and elasticity. The interplay between these collagens ensures the continuous renewal and repair of skin tissues essential for wound healing and overall skin health.

Collagen VII mutations or deficiencies lead to dystrophic epidermolysis bullosa (DEB) a genetic skin condition characterized by severe blistering. This disorder arises from defective anchoring fibril formation causing skin layers to separate easily. The interplay between collagen VII and other collagens like collagen type I becomes compromised exacerbating tissue fragility. Understanding these interactions provides insight into targeted treatments and management strategies for DEB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

See full target information Collagen alpha-1(VII) chain

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cell journal 25:665-673 PubMed37865875

2023

CRISPR/Cas9-Mediated Generation of -Deficient Keratinocyte Model of Recessive Dystrophic Epidermolysis Bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Farzad Alipour,Mana Ahmadraji,Elham Yektadoost,Parvaneh Mohammadi,Hossein Baharvand,Mohsen Basiri

Molecular therapy : the journal of the American Society of Gene Therapy 30:2664-2679 PubMed35690907

2022

Therapeutic base editing and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Ah Hong,Song-Ee Kim,A-Young Lee,Gue-Ho Hwang,Jong Hoon Kim,Hiroaki Iwata,Soo-Chan Kim,Sangsu Bae,Sang Eun Lee

Experimental dermatology 31:1065-1075 PubMed35243691

2022

Genes and compounds that increase type VII collagen expression as potential treatments for dystrophic epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth L Thompson,Michael Pickett-Leonard,Megan J Riddle,Weili Chen,Frank W Albert,Jakub Tolar

Journal of clinical medicine 11: PubMed35207382

2022

Cryopreservation in a Standard Freezer: -28 °C as Alternative Storage Temperature for Amniotic Membrane Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Joana Witt,Luis Grumm,Sabine Salla,Gerd Geerling,Johannes Menzel-Severing

Scientific reports 12:952 PubMed35046484

2022

Trehangelins ameliorate inflammation-induced skin senescence by suppressing the epidermal YAP-CCN1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mami Yokota,Yoshiyuki Kamiya,Tamie Suzuki,Shinsuke Ishikawa,Akira Takeda,Shinya Kondo,Takeshi Tohgasaki,Takuji Nakashima,Yoko Takahashi,Satoshi Ōmura,Tetsuhito Sakurai

International journal of molecular sciences 22: PubMed33916959

2021

hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination.

Applications

Unspecified application

Species

Unspecified reactive species

Nadezhda A Evtushenko,Arkadii K Beilin,Erdem B Dashinimaev,Rustam H Ziganshin,Anastasiya V Kosykh,Maxim M Perfilov,Alexandra L Rippa,Elena V Alpeeva,Andrey V Vasiliev,Ekaterina A Vorotelyak,Nadya G Gurskaya

International journal of molecular sciences 22: PubMed33670258

2021

Signatures of Dermal Fibroblasts from RDEB Pediatric Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Arkadii K Beilin,Nadezhda A Evtushenko,Daniil K Lukyanov,Nikolay N Murashkin,Eduard T Ambarchian,Alexander A Pushkov,Kirill V Savostyanov,Andrey P Fisenko,Olga S Rogovaya,Andrey V Vasiliev,Ekaterina A Vorotelyak,Nadya G Gurskaya

Nature communications 11:4239 PubMed32843640

2020

Single cell transcriptomics of human epidermis identifies basal stem cell transition states.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiong Wang,Michael L Drummond,Christian F Guerrero-Juarez,Eric Tarapore,Adam L MacLean,Adam R Stabell,Stephanie C Wu,Guadalupe Gutierrez,Bao T That,Claudia A Benavente,Qing Nie,Scott X Atwood

JCI insight 5: PubMed32369451

2020

Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Nesrine Boukhedouni,Christina Martins,Anne-Sophie Darrigade,Claire Drullion,Jérôme Rambert,Christine Barrault,Julien Garnier,Clément Jacquemin,Denis Thiolat,Fabienne Lucchese,Franck Morel,Khaled Ezzedine,Alain Taieb,François-Xavier Bernard,Julien Seneschal,Katia Boniface

GeroScience 41:861-869 PubMed31761958

2019

Topical rapamycin reduces markers of senescence and aging in human skin: an exploratory, prospective, randomized trial.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Lee Chung,Ibiyonu Lawrence,Melissa Hoffman,Dareen Elgindi,Kumar Nadhan,Manali Potnis,Annie Jin,Catlin Sershon,Rhonda Binnebose,Antonello Lorenzini,Christian Sell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen VII antibody - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Collagen VII antibody - BSA and Azide free (AB93350)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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