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AB6312

Anti-Collagen VII antibody [LH7.2]

5

(3 Reviews)

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(13 Publications)

Anti-Collagen VII antibody [LH7.2] (ab6312) is a mouse monoclonal antibody detecting Collagen VII in IHC-Fr. Suitable for Human.

- Trusted since 2000

View Alternative Names

Collagen alpha-1(VII) chain, Long-chain collagen, LC collagen, COL7A1

1 Images
Immunohistochemistry (Frozen sections) - Anti-Collagen VII antibody [LH7.2] (AB6312)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Collagen VII antibody [LH7.2] (AB6312)

Immunohistochemistry analysis of frozen acetone human tonsil sections labeling Collagen VII with ab6312 at 3 μg/mL followed by FITC-Anti-Mouse IgG.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LH7.2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Human Collagen alpha-1(VII) chain.

Q02388

Epitope

Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer.Immunoelectron microscopy shows antibody binding localised at the inferior border of the lamina densa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1.5-3 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Collagen VII antibody [LH7.2] (ab6312) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr) in Human samples.

Trusted by the scientific community
Anti-Collagen VII [LH7.2] (ab6312) was first used in a scientific publication in 2000 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Purified from Tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen VII also known as collagen type VII or collagen 7 is an important structural component of the extracellular matrix. It measures approximately 290 kDa in mass. Researchers identified it chiefly in stratified squamous epithelia and the basement membrane zone of the skin. Collagen VII plays a mechanical role as anchoring fibrils maintaining skin integrity by attaching the dermal tissue to the epidermal basement membrane. In marmoset dermal tissue similar functions are observed indicating a conserved role in primates.
Biological function summary

Collagen VII forms part of a larger complex known as anchoring fibrils. These fibrils play an essential role in providing structural support and maintaining mechanical stability of tissues. The protein enhances the connection between the epidermis and dermis preventing separation between these layers. Its presence signifies the maintenance of tissue integrity and resilience critical for normal skin function.

Pathways

Collagen type VII interacts within the epidermal basement membrane zone part of the broader extracellular matrix (ECM) remodeling pathway. It collaborates with other ECM components like collagen type I and collagen type III which all contribute to skin strength and elasticity. The interplay between these collagens ensures the continuous renewal and repair of skin tissues essential for wound healing and overall skin health.

Collagen VII mutations or deficiencies lead to dystrophic epidermolysis bullosa (DEB) a genetic skin condition characterized by severe blistering. This disorder arises from defective anchoring fibril formation causing skin layers to separate easily. The interplay between collagen VII and other collagens like collagen type I becomes compromised exacerbating tissue fragility. Understanding these interactions provides insight into targeted treatments and management strategies for DEB.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
See full target information Collagen alpha-1(VII) chain
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 9:e10640 PubMed39036091

2024

Highly efficient CRISPR/Cas9-mediated exon skipping for recessive dystrophic epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Alex du Rand,John Hunt,Christopher Samson,Evert Loef,Chloe Malhi,Sarah Meidinger,Chun-Jen Jennifer Chen,Ashley Nutsford,John Taylor,Rod Dunbar,Diana Purvis,Vaughan Feisst,Hilary Sheppard

Pharmaceutics 16: PubMed38399319

2024

Dual Glyoxalase-1 and β-Klotho Gene-Activated Scaffold Reduces Methylglyoxal and Reprograms Diabetic Adipose-Derived Stem Cells: Prospects in Improved Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Pang,Ashang L Laiva,Noof Z Sulaiman,Priya Das,Fergal J O'Brien,Michael B Keogh

The Journal of dermatology 50:1194-1198 PubMed37082790

2023

Usefulness of immunofluorescence overlay antigen mapping in the identification of autoantigen in anti-p200 pemphigoid.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Kitayama,Teruhiko Makino,Masao Hayashi,Megumi Mizawa,Norito Ishii,Takashi Hashimoto,Tadamichi Shimizu

Science advances 9:eade2514 PubMed36706190

2023

Engineering edgeless human skin with enhanced biomechanical properties.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Pappalardo,David Alvarez Cespedes,Shuyang Fang,Abigail R Herschman,Eun Young Jeon,Kristin M Myers,Jeffrey W Kysar,Hasan Erbil Abaci

JCI insight 6: PubMed33491668

2021

Intravenous allogeneic umbilical cord blood-derived mesenchymal stem cell therapy in recessive dystrophic epidermolysis bullosa patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Eun Lee,Seung-Ju Lee,Song-Ee Kim,Kinam Kim,Boyoung Cho,Kyounghwan Roh,Soo-Chan Kim

Communications biology 3:637 PubMed33127987

2020

Tissue-scale tensional homeostasis in skin regulates structure and physiological function.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Kimura,Ayako Tsuchiya,Miho Ogawa,Miki Ono,Nao Suda,Kaori Sekimoto,Makoto Takeo,Takashi Tsuji

Nature communications 10:2759 PubMed31227717

2019

Identification of HIV transmitting CD11c human epidermal dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kirstie M Bertram,Rachel A Botting,Heeva Baharlou,Jake W Rhodes,Hafsa Rana,J Dinny Graham,Ellis Patrick,James Fletcher,Toby M Plasto,Naomi R Truong,Caroline Royle,Chloe M Doyle,Orion Tong,Najla Nasr,Laith Barnouti,Mark P Kohout,Andrew J Brooks,Michael P Wines,Peter Haertsch,Jake Lim,Martijn P Gosselink,Grahame Ctercteko,Jacob D Estes,Melissa J Churchill,Paul U Cameron,Eric Hunter,Muzlifah A Haniffa,Anthony L Cunningham,Andrew N Harman

Journal of tissue engineering and regenerative medicine 13:1362-1374 PubMed31067012

2019

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment.

Applications

Unspecified application

Species

Unspecified reactive species

Maia M Alexaline,Brice Magne,Amparo Zuleta Rodríguez,Muriel Nivet,Daniel Bacqueville,Jean-Jacques Lataillade,Marina Trouillas

Archives of dermatological research 311:647-652 PubMed31168656

2019

Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently: towards improved wound care.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy Bage,Trevor Edymann,Anthony D Metcalfe,Baljit Dheansa,Lubinda Mbundi

Tissue engineering. Part C, Methods 25:334-343 PubMed31007132

2019

Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function.

Applications

Unspecified application

Species

Unspecified reactive species

Kristy Derr,Jinyun Zou,Keren Luo,Min Jae Song,G Sitta Sittampalam,Chao Zhou,Sam Michael,Marc Ferrer,Paige Derr
View all publications
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Product promise

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