AB58632

Anti-Collagen X antibody

Name: Anti-Collagen X antibody. Rabbit polyclonal (ab58632) | Abcam
Brand: Abcam Limited
SKU: AB58632
Price: 495 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(294 Publications)

Rabbit Polyclonal Collagen X antibody. Suitable for WB and reacts with Human samples. Cited in 294 publications.

View Alternative Names

Collagen alpha-1(X) chain, COL10A1

1 Images

Unknown

Western blot - Anti-Collagen X antibody (AB58632)

The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.

All lanes:

Western blot - Anti-Collagen X antibody (ab58632) at 1/500 dilution

All lanes:

HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 66 kDa

Observed band size: 37 kDa,50 kDa,64 kDa,66 kDa,98 kDa

true

Exposure time: 150s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Specificity

ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/300", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.

Biological function summary

COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.

Pathways

COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.

Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

See full target information COL10A1

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Publications (294)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:880 PubMed41063183

2025

Roles of TGF-β in the therapeutic potential of hypoxic mesenchymal stem cells for treating osteoarthritis in a Rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

En-Rung Chiang,Kun-Hui Chen,Hsuan-Hsiao Ma,Jung-Pan Wang,Hsiao-Li Ma

Cell death & disease 16:678 PubMed41053113

2025

SDC4 drives fibrotic remodeling of the intervertebral disc under altered spinal loading.

Applications

Unspecified application

Species

Unspecified reactive species

Kimheak Sao,Makarand V Risbud

Frontiers in immunology 16:1626337 PubMed40959091

2025

Single-cell RNA-seq reveals a repair pattern in cystic lesions in steroid induced osteonecrosis of the femoral head.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Peng,Jiaqing Tian,Kun Lin,Weihua Fang,Fangming Yao,Xiaoqiang Yang,Wenyuan Hou,Fangjun Xiao,Shun Lu,Wei He,Mincong He,Huan Xiao,Qiushi Wei

Aging 17:2369-2391 PubMed40932696

2025

overexpression induces early onset of intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Fukunaga,Martha Elena Diaz-Hernandez,John G Heller,Changli Zhang,Hicham Drissi

Traffic (Copenhagen, Denmark) 26:e70013 PubMed40747612

2025

FGF Signaling Promotes Lysosome Biogenesis in Chondrocytes via the Mannose Phosphate Receptor Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cinque,Maria Iavazzo,Gennaro Di Bonito,Elena Polishchuk,Rossella De Cegli,Carmine Settembre

Nature communications 16:5996 PubMed40595596

2025

The loss of OPA1 accelerates intervertebral disc degeneration and osteoarthritis in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vedavathi Madhu,Miriam Hernandaz-Meadows,Ashley Coleman,Kimheak Sao,Kameron Inguito,Owen Haslam,Paige K Boneski,Hiromi Sesaki,Ruteja A Barve,John A Collins,Makarand V Risbud

Journal of orthopaedic translation 52:387-403 PubMed40476065

2025

Vitamins D and K jointly protect against osteoarthritis via regulating OSCAR during osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Qianhua Ou,Hong Huang,Delong Li,Jianmao Chen,Song Xue,Zuoqing Zhou,Guangfeng Ruan,Changhai Ding

Bone research 13:57 PubMed40442075

2025

RUNX2 is essential for maintaining synchondrosis chondrocytes and cranial base growth.

Applications

Unspecified application

Species

Unspecified reactive species

Shawn A Hallett,Ashley Dixon,Isabella Marrale,Lena Batoon,José Brenes,Annabelle Zhou,Ariel Arbiv,Vesa Kaartinen,Benjamin Allen,Wanida Ono,Renny T Franceschi,Noriaki Ono

Bone research 13:50 PubMed40335469

2025

Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pranay Ramteke,Bahiyah Watson,Mallory Toci,Victoria A Tran,Shira Johnston,Maria Tsingas,Ruteja A Barve,Ramkrishna Mitra,Richard F Loeser,John A Collins,Makarand V Risbud

Bone & joint research 14:356-367 PubMed40237708

2025

Carpaine ameliorates synovial inflammation by promoting p65 degradation and inhibiting the NF-κB signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Zhang,Ziyang Li,Zhicheng Zhang,Haobin Li,Zihao Yao,Haiyan Zhang,Chang Zhao,Xiaochun Bai,Chenglong Pan,Daozhang Cai,Chun Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen X antibody

Western blot - Anti-Collagen X antibody (AB58632)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (294)

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