AB49945

Anti-Collagen X antibody [COL-10]

Name: Anti-Collagen X antibody [COL-10] (ab49945) | Abcam
Brand: Abcam Limited
SKU: AB49945
Price: 505 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(119 Publications)

Anti-Collagen X antibody [COL-10] (ab49945) is a mouse monoclonal antibody detecting Collagen X in IHC-P, ICC/IF. Suitable for Human.

- Over 90 publications
- Trusted since 2007

View Alternative Names

Collagen alpha-1(X) chain, COL10A1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (AB49945)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (AB49945)

ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.

This image was generated using the ascites version of the product.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (AB49945)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (AB49945)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

COL-10

Isotype

IgM

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Epitope

Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Collagen X antibody [COL-10] (ab49945) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-Collagen X [COL-10] (ab49945) was first used in a scientific publication in 2007 and has been cited over 90 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 0.142% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.

Biological function summary

COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.

Pathways

COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.

Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

See full target information COL10A1

style

left-column

Publications (119)

Recent publications for all applications. Explore the full list and refine your search

Advanced functional materials 34: PubMed40800235

2025

Functionalized Annealed Microgels for Spatial Control of Osteogenic and Chondrogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy M Lowen,Erika E Wheeler,Nathan K Shimamoto,David H Ramos-Rodriguez,Katherine H Griffin,Gabriella C Bond,J Kent Leach

iScience 28:112608 PubMed40502710

2025

The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Wang,Haodong Li,Dong Fu,Yiming Zheng,Chuang Qian,Lin Li,Maoxiang Qian,Dahui Wang

Bioengineering (Basel, Switzerland) 12: PubMed40428164

2025

The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell Fibrochondrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ka Yu Carissa Kwan,Ke Li,Yu Yang Wang,Wai Yi Tse,Chung Yan Tong,Xu Zhang,Dan Michelle Wang,Dai Fei Elmer Ker

Journal of inflammation research 18:4233-4250 PubMed40129871

2025

Systemic Lupus Erythematosus Stimulates Chondrocyte Pyroptosis to Aggravate Arthritis via Suppression of NRF-2/KEAP-1 and NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuchao Shen,Xuliang Fang,Helou Zhang,Tingting Lang,Fangda Fu,Yu Du,Taotao Xu,Hongting Jin,Peijian Tong,Chengliang Wu,Changfeng Hu,Hongfeng Ruan

Biomaterials research 29:0152 PubMed39990979

2025

Insulin-Like Growth Factor 2 Secreted from Mesenchymal Stem Cells with High Glutathione Levels Alleviates Osteoarthritis via Paracrine Rejuvenation of Senescent Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gun Hee Cho,Hyun Cheol Bae,Yu Jeong Lee,Ha Ru Yang,Hyewon Kang,Hee Jung Park,Sun Young Wang,You Jung Kim,Heun-Soo Kang,In Gyu Kim,Byung Sun Choi,Hyuk-Soo Han

Materials today. Bio 31:101491 PubMed39896288

2025

Deciphering cartilage neuro-immune interactions and innervation profile through 3D engineered osteoarthritic micropathophysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Emine Kahraman,Daniela Vasconcelos,Beatriz Ribeiro,Ana Carolina Monteiro,Enzo Mastromatteo,Andrea Bortolin,Marina Couto,Laura Boschis,Meriem Lamghari,Estrela Neto

Journal of cellular and molecular medicine 28:e70249 PubMed39656597

2024

COL10A1 Facilitates Prostate Cancer Progression by Interacting With INHBA to Activate the PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Jiang,Li-Zhe Xu,Fan Cheng,Jin-Zhuo Ning

Annals of biomedical engineering 53:597-611 PubMed39602036

2024

Engineered Osteochondral Scaffolds with Bioactive Cartilage Zone for Enhanced Articular Cartilage Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra A Golebiowska,Jonathon T Intravaia,Vinayak Sathe,Sangamesh G Kumbar,Syam P Nukavarapu

ACS biomaterials science & engineering 10:6441-6450 PubMed39240109

2024

Rapidly Degrading Hydrogels to Support Biofabrication and 3D Bioprinting Using Cartilage Microtissues.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela S Kronemberger,Francesca D Spagnuolo,Aliaa S Karam,Kaoutar Chattahy,Kyle J Storey,Daniel J Kelly

Bioactive materials 40:306-317 PubMed38978806

2024

Bioceramic-mediated chondrocyte hypertrophy promotes calcified cartilage formation for rabbit osteochondral defect repair.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel H Koh,Junhee Kim,Jeong-Uk Kim,Seunghyun L Kim,Arun Kumar Rajendran,Seunghun S Lee,Heesoo Lee,Joo Hyun Kim,Ji Hoon Jeong,Yongsung Hwang,Jong Woo Bae,Nathaniel S Hwang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen X antibody [COL-10]

Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (AB49945)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (AB49945)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (119)

Product promise