Skip to main content

Rabbit Recombinant Monoclonal Collagen X antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 23 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Expected
Rat
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/2000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/1000 - 1/2000

Notes

-

Target data

Function

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal Collagen X antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 23 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR13044

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.

Activity summary

Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.

Pathways

COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.

Associated diseases and disorders

Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Collagen X antibody [EPR13044] (ab182563), expandable thumbnail

    Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)

    Exposure time
    Lane 1: 6 seconds
    Lane 2: 70 seconds
    Lane 3 to 5: 10 seconds

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.

    All lanes: Western blot - Anti-Collagen X antibody [EPR13044] (AB182563) at 1/1000 dilution

    Lane 1: Human fetal skin tissue lysates at 15 µg

    Lane 2: Rat skin tissue lysates at 15 µg

    Lane 3: Mouse cartilage tissue lysates at 15 µg

    Lane 4: Human cartilage tissue lysates at 15 µg

    Lane 5: Rat cartilage tissue lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 66 kDa

    Observed band size: 66 kDa

  • Western blot - Anti-Collagen X antibody [EPR13044] (ab182563), expandable thumbnail

    Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)

    All lanes: Western blot - Anti-Collagen X antibody [EPR13044] (AB182563) at 1/1000 dilution

    Lane 1: Human fetal skin lysate at 20 µg

    Lane 2: Human placenta lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa

    Observed band size: 66 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com