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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal Collagen X antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 23 publications.
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Mouse | Expected |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Collagen alpha-1(X) chain, COL10A1
Rabbit Recombinant Monoclonal Collagen X antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 23 publications.
Collagen alpha-1(X) chain, COL10A1
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR13044
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.
Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.
COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.
Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Exposure time
Lane 1: 6 seconds
Lane 2: 70 seconds
Lane 3 to 5: 10 seconds
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.
All lanes: Western blot - Anti-Collagen X antibody [EPR13044] (AB182563) at 1/1000 dilution
Lane 1: Human fetal skin tissue lysates at 15 µg
Lane 2: Rat skin tissue lysates at 15 µg
Lane 3: Mouse cartilage tissue lysates at 15 µg
Lane 4: Human cartilage tissue lysates at 15 µg
Lane 5: Rat cartilage tissue lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
All lanes: Western blot - Anti-Collagen X antibody [EPR13044] (AB182563) at 1/1000 dilution
Lane 1: Human fetal skin lysate at 20 µg
Lane 2: Human placenta lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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