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AB260040

Anti-Collagen X antibody [EPR22920-211]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

0

(1 Review)

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(24 Publications)

Anti-Collagen X antibody [EPR22920-211] (ab260040) is a rabbit monoclonal antibody detecting Collagen X in IHC-P, IHC-Fr. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Collagen alpha-1(X) chain, COL10A1

2 Images
Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Trition X-100 permeabilized mouse trachea tissue labeling Collagen X with ab260040 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on hyaline cartilage of mouse trachea (PMID : 22859705). The nuclear counterstain is DAPI (blue).

Secondary antibody only control : ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

Immunohistochemical analysis of paraffin-embedded mouse articular cartilage tissue labeling Collagen X with ab260040 at 1/1000 dilution (0.57μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the hypertrophic zone in the growth plate of mouse bone (PMID : 22859705). The section was incubated with ab260040 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : A ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Carrier free

    Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22920-211

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Collagen X antibody [EPR22920-211] (ab260040) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR22920-211] also available for your convenience: ab260040, Carrier free - ab264571

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.
Biological function summary

COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.

Pathways

COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.

Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
See full target information COL10A1
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 54:492-508 PubMed40955375

2025

Inherently bioactive iron-chelating Poly (N-acryloyl 2-glycine)/chitosan hydrogel scaffolds orchestrating dual hypoxic-immune microenvironment for functional meniscus regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Bingbing Xu,Jing Ye,Shitang Song,Xueyu Dou,Chao Li,Xing Wang,Jia-Kuo Yu

Chinese medicine 20:142 PubMed40890747

2025

Sodium tanshinone IIA sulfonate alleviates osteoarthritis through targeting SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Xu,Xulei Sun,Xiao Ma,Zixuan Qin,Xin Gao,Xinxin Jin,Hongzhi Sun

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413970 PubMed39936497

2025

N6-Methyladenosine-Modified circSMAD4 Prevents Lumbar Instability Induced Cartilage Endplate Ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Hanwen Li,Yingchuang Tang,Sihan Hu,Xingbang Ruan,Junxin Zhang,Yihan Shi,Liang Qiu,Huilin Yang,Kai Zhang,Hao Chen,Kangwu Chen

Experimental biology and medicine (Maywood, N.J.) 249:10066 PubMed39911634

2025

Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Teitelbaum,Maya D Culbertson,Charlene Wetterstrand,J Patrick O'Connor

Communications biology 7:1380 PubMed39443661

2024

Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.

Applications

Unspecified application

Species

Unspecified reactive species

Zibin Zhou,Xiaoyu Mao,Chun Jiang,Wenguo Li,Tiantian Zhou,Meng Liu,Shijin Sun,Mengting Wang,Ningzheng Dong,Qingyu Wu,Haibin Zhou

Scientific reports 14:20136 PubMed39209899

2024

MiR-322-5p is involved in regulating chondrocyte proliferation and differentiation in offspring's growth plate of maternal gestational diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Qian,Xianlong Chen,Simiao Wang,Yeyin Zhong,Min Liu,Guang Wang,Xuesong Yang,Xin Cheng

eLife 12: PubMed38896465

2024

Senescence of endplate osteoclasts induces sensory innervation and spinal pain.

Applications

Unspecified application

Species

Unspecified reactive species

Dayu Pan,Kheiria Gamal Benkato,Xuequan Han,Jinjian Zheng,Vijay Kumar,Mei Wan,Junying Zheng,Xu Cao

Frontiers in cell and developmental biology 12:1360041 PubMed38895158

2024

Sclerostin modulates mineralization degree and stiffness profile in the fibrocartilaginous enthesis for mechanical tissue integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Shinsei Yambe,Yuki Yoshimoto,Kazutaka Ikeda,Koichiro Maki,Aki Takimoto,Akihide Tokuyama,Shinnosuke Higuchi,Xinyi Yu,Kenta Uchibe,Shigenori Miura,Hitomi Watanabe,Tetsushi Sakuma,Takashi Yamamoto,Kotaro Tanimoto,Gen Kondoh,Masataka Kasahara,Toshihide Mizoguchi,Denitsa Docheva,Taiji Adachi,Chisa Shukunami
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com