AB227945

Anti-Collagen XI alpha 2/COL11A2 antibody - Membrane Vesicle Marker

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(2 Publications)

Rabbit Polyclonal Collagen XI alpha 2/COL11A2 antibody. Membrane Vesicle marker. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Collagen alpha-2(XI) chain.

View Alternative Names

Collagen alpha-2(XI) chain, COL11A2

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Supplier Data

Western blot - Anti-Collagen XI alpha 2/COL11A2 antibody - Membrane Vesicle Marker (AB227945)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Collagen XI alpha 2/COL11A2 antibody - Membrane Vesicle Marker (ab227945) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Collagen XI alpha 2/COL11A2 transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 172 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human Collagen alpha-2(XI) chain. The exact immunogen used to generate this antibody is proprietary information.

P13942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen XI alpha 2 (COL11A2) also known as alpha-2 chain of type XI collagen plays a mechanical role in the formation of collagen fibrils found in cartilage. This protein has a mass of approximately 178 kDa. COL11A2 is expressed in cartilaginous tissues including articular cartilage and intervertebral discs. Its structural properties help in maintaining the tensile strength and integrity of extracellular matrices in these tissues contributing to skeletal development and joint function.

Biological function summary

Collagen XI alpha 2 is integral to the development and maintenance of cartilage tissue. It is a component of the collagen type XI complex which includes collagen fibers composed of three polypeptide chains: alpha-1 alpha-2 and alpha-3. These fibers regulate the size and spacing of collagen fibrils during cartilage formation ensuring proper bone growth and maintenance. COL11A2 works in coordination with other collagen types such as collagen II and IX to ensure stability and structural cohesion within the cartilage matrix.

Pathways

Collagen XI alpha 2 is essential in the assembly and organization of collagen fibrils within the extracellular matrix which plays a vital role in the bone morphogenetic protein (BMP) signaling pathway and the TGF-beta (transforming growth factor-beta) pathway. These pathways facilitate various cellular processes including cell proliferation and differentiation. COL11A2 interacts with proteins like collagen type II (COL2A1) to modulate these pathways ensuring proper cartilage and skeletal tissue development.

COL11A2 mutations can lead to conditions such as Stickler syndrome and Otospondylomegapiphyseal Dysplasia (OSMED). Stickler syndrome affects connective tissues leading to abnormalities in the eyes bones and ears while OSMED results in skeletal dysplasia. Both disorders relate to structural anomalies in cartilage. The protein shares a linkage with collagen type II in these conditions as alterations in the COL11A2 gene can disrupt the normal interaction between these collagen molecules further impacting skeletal growth and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.

See full target information Collagen alpha-2(XI) chain

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:105173 PubMed40548267

2025

Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Tang,Cheng-Fen Wang,Jue Wang,Xiao-Tao Jing,Jing Ma

Journal of cellular and molecular medicine 28:e18395 PubMed38774995

2024

Analysis of the effects of M2 macrophage-derived PDE4C on the prognosis, metastasis and immunotherapy benefit of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Runsang Pan,Rui Hu,Hao Zhang,Shan Lei,Lu Zhang,Changhua Zhou,Zhirui Zeng,Xiaobin Tian,Quan Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Collagen XI alpha 2/COL11A2 antibody - Membrane Vesicle Marker

Western blot - Anti-Collagen XI alpha 2/COL11A2 antibody - Membrane Vesicle Marker (AB227945)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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