AB28440

Anti-Collagen XVII antibody

Name: Anti-Collagen XVII antibody (ab28440) | Abcam
Brand: Abcam Limited
SKU: AB28440
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal Collagen XVII antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL17A1 aa 500-550.

View Alternative Names

BP180, BPAG2, COL17A1, Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, ICC, WB, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human COL17A1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q9UMD9

Specificity

ab28440 may also detect GST

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen XVII also known as collagen 17 or 17a1 collagen is a transmembrane protein that primarily functions as a structural component in epithelial cells. It weighs approximately 180 kDa and is mainly expressed in the basement membrane of stratified epithelia. Collagen XVII forms hemidesmosomes which are essential for the attachment of epithelial cells to the underlying basement membrane. The protein ensures mechanical stability and integrity of tissues where it is found.

Biological function summary

Collagen XVII contributes to the maintenance of cell adhesion and tissue architecture. It acts as a component of the hemidesmosome complex connecting the extracellular matrix to the cytoskeleton through integrins. This interaction promotes structural stability and supports tissue repair processes. The protein's expression reaches high levels in skin mucous membranes and the cornea playing a significant role in maintaining the barrier function of these tissues.

Pathways

Collagen XVII interacts with cellular signaling pathways related to cell adhesion and migration. The protein is involved in the integrin-mediated signaling pathway which regulates cell survival and differentiation. Additionally it participates in the basement membrane pathway collaborating with proteins such as laminin and bullous pemphigoid antigens to maintain tissue cohesion and repair.

Collagen XVII is closely associated with bullous pemphigoid and junctional epidermolysis bullosa. In bullous pemphigoid antibodies target collagen XVII leading to skin blistering due to weakened cell adhesion. In junctional epidermolysis bullosa mutations in the gene encoding collagen XVII disrupt its function resulting in fragile skin and mucosa. These disorders highlight the important role this protein plays in maintaining epithelial tissue integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.. The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.

See full target information COL17A1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical materials research. Part A 111:1021-1030 PubMed36621832

2023

Implant surface physicochemistry affects keratinocyte hemidesmosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Michail Raptopoulos,Nicholas G Fischer,Conrado Aparicio

Acta biomaterialia 141:70-88 PubMed34971784

2022

Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas G Fischer,Alexandra C Kobe,Jinhong Dai,Jiahe He,Hongning Wang,John A Pizarek,David A De Jong,Zhou Ye,Shengbin Huang,Conrado Aparicio

ACS biomaterials science & engineering 6:4929-4939 PubMed32953986

2020

Keratinocyte-Specific Peptide-Based Surfaces for Hemidesmosome Upregulation and Prevention of Bacterial Colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas G Fischer,Dina G Moussa,Erik P Skoe,David A De Jong,Conrado Aparicio

Coatings (Basel, Switzerland) 10: PubMed32855816

2020

Surface Immobilization Chemistry of a Laminin-Derived Peptide Affects Keratinocyte Activity.

Applications

ICC

Species

Human

Nicholas G Fischer,Jiahe He,Conrado Aparicio

Journal of biomedical science 27:5 PubMed31928533

2020

Involvement of collagen XVII in pluripotency gene expression and metabolic reprogramming of lung cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Shui Hsu,Chen-Chi Liu,Jiun-Han Lin,Tien-Wei Hsu,Jyuan-Wei Hsu,Anna Fen-Yau Li,Shih-Chieh Hung

Biomaterials science 6:1936-1945 PubMed29850754

2018

Peptide coatings enhance keratinocyte attachment towards improving the peri-implant mucosal seal.

Applications

Unspecified application

Species

Unspecified reactive species

Vasiliki P Koidou,Prokopios P Argyris,Erik P Skoe,Juliana Mota Siqueira,Xi Chen,Lei Zhang,James E Hinrichs,Massimo Costalonga,Conrado Aparicio

Nature communications 7:11798 PubMed27306323

2016

Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells.

Applications

IF, IHC, WB

Species

Unspecified reactive species, Unspecified reactive species, Unspecified reactive species

Chen-Chi Liu,Shih-Pei Lin,Han-Shui Hsu,Shung-Haur Yang,Chiu-Hua Lin,Muh-Hwa Yang,Mien-Chie Hung,Shih-Chieh Hung

European cells & materials 29:237-49 PubMed25890596

2015

Intraosseous transcutaneous amputation prostheses versus dental implants: a comparison between keratinocyte and gingival epithelial cell adhesion in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

C J Pendegrass,H T Lancashire,C Fontaine,G Chan,P Hosseini,G W Blunn

Human mutation 36:463-73 PubMed25676728

2015

Mutations in collagen, type XVII, alpha 1 (COL17A1) cause epithelial recurrent erosion dystrophy (ERED).

Applications

Unspecified application

Species

Unspecified reactive species

Frida Jonsson,Berit Byström,Alice E Davidson,Ludvig J Backman,Therese G Kellgren,Stephen J Tuft,Timo Koskela,Patrik Rydén,Ola Sandgren,Patrik Danielson,Alison J Hardcastle,Irina Golovleva

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen XVII antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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