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AB243106

Anti-Collagenase ColA antibody [cp-02]

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(1 Publication)

Mouse Monoclonal COLA antibody. Suitable for WB and reacts with Clostridium perfringens, Clostridium histolyticum samples. Cited in 1 publication.

View Alternative Names

CPE0173, colA, Collagenase ColA, 120 kDa collagenase, Microbial collagenase

1 Images
Western blot - Anti-Collagenase ColA antibody [cp-02] (AB243106)
  • WB

Supplier Data

Western blot - Anti-Collagenase ColA antibody [cp-02] (AB243106)

All lanes:

Western blot - Anti-Collagenase ColA antibody [cp-02] (ab243106) at 1/1000 dilution

Lane 1:

Purified collagenase of C. histolyticum

Lane 2:

Culture supernatant of C. perfringens

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

cp-02

Isotype

IgM

Carrier free

No

Reacts with

Clostridium perfringens, Clostridium histolyticum

Applications

WB

applications

Specificity

with collagenases of Clostridium perfringens and C.histolyticcum.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Clostridium histolyticum": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Clostridium perfringens": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified using Ab-Capture for IgM.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagenase ColA also known as Collagenase G is an enzyme that breaks down collagen in the extracellular matrix. This enzyme belongs to the family of metalloproteases and has a mass of approximately 116 kDa. Collagenase ColA is expressed in a variety of bacteria notably in Clostridium histolyticum. It plays an important role in tissue remodeling and bacterial invasion by facilitating the breakdown of collagen which is the most abundant protein in the human body.
Biological function summary

Collagenase ColA enables bacterial pathogens to penetrate host tissues by degrading structural barriers composed of collagen. It is not part of a large complex but functions individually to exert its collagen-degrading action. This ability to degrade collagen makes it essential for bacterial infection processes and host tissue infiltration highlighting its significance in microbial pathogenicity.

Pathways

Collagen degradation involves the participation of Collagenase ColA impacting processes like connective tissue breakdown and remodeling. It collaborates with other proteases and is involved in extracellular matrix pathways. The action of this enzyme also links with protease-activated receptor signaling pathways which can involve other related proteins such as gelatinases and stromelysins that share collagenolytic activities.

Increased activity of Collagenase ColA is associated with diseases like necrotizing fasciitis and bacterial infections leading to cellulitis. These disorders often result due to the enzyme's capability to assist bacterial spread by degrading host tissue barriers. Moreover increased collagenase activity can link to other proteins like matrix metalloproteinases which are often upregulated in inflammatory conditions and tissue degradation diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (By similarity).
See full target information colA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1367432 PubMed38994364

2024

The mammosphere-derived epithelial cell secretome modulates neutrophil functions in the bovine model.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Harman,Anja Sipka,Kelly A Oxford,Leane Oliveira,Lucas Huntimer,Daryl V Nydam,Gerlinde R Van de Walle
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