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Rabbit Recombinant Monoclonal ARHGEF9 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-Collybistin antibody [EPR28024-32] (AB316963), expandable thumbnail
  • Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (AB316963), expandable thumbnail
  • Western blot - Anti-Collybistin antibody [EPR28024-32] (AB316963), expandable thumbnail
  • Western blot - Anti-Collybistin antibody [EPR28024-32] (AB316963), expandable thumbnail
  • Western blot - Anti-Collybistin antibody [EPR28024-32] (AB316963), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ARHGEF9 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28024-32

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collybistin also known as ARHGEF9 is a protein that functions as a guanine nucleotide exchange factor (GEF) primarily for the small GTPase Cdc42. This mechanism is essential in the regulation of actin cytoskeleton dynamics with an approximate molecular mass of 46 kDa. You can find Collybistin mainly in the brain where it plays a significant role in synaptic formation and function particularly in the postsynaptic sites of inhibitory synapses.

Biological function summary

Collybistin plays a critical role in clustering and trafficking of specific proteins required for synapse maturation and stability. It participates as a part of a larger complex that includes key synaptic proteins like Gephyrin a scaffolding protein important for neurotransmitter receptor localization. This interaction between Collybistin and synaptic components ensures proper synaptic inhibitory signaling by controlling receptor clustering and distribution.

Pathways

Collybistin is essential within the pathways associated with synaptic vesicle function and cytoskeletal dynamics. This protein interacts with important pathways like the Rho GTPase signaling pathway and Cdc42 effector pathways which are vital for cytoskeletal rearrangements. Through these pathways Collybistin engages with proteins such as F-actin influencing synaptic plasticity and neural connectivity by altering actin dynamics.

Associated diseases and disorders

Collybistin is linked to neurological conditions with particular relevance to epilepsy. Mutations in the Collybistin encoding gene ARHGEF9 have been associated with forms of epilepsy and intellectual disabilities. In these conditions disrupted interactions between Collybistin and other synaptic proteins like Gephyrin can lead to altered synaptic signaling illustrating the importance of its proper function in neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963), expandable thumbnail

    Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963)

    This antibody does not cross-react with human ARHG4.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963) at 1/1000 dilution

    Lane 1: His-tagged human Collybistin fragment at 10 ng

    Lane 2: His-tagged human ARHG4 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 43 kDa

    Exposure time: 6s

  • Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (ab316963), expandable thumbnail

    Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (ab316963)

    Collybistin was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab316963 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316963 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lane 2: ab316963 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316963 in SH-SY5Y whole cell lysate

    All lanes: Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (ab316963) at 1/30 dilution

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 84s

  • Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963), expandable thumbnail

    Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963)

    Negative control: HUVEC (PMID: 19911011).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963) at 1/1000 dilution

    Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963), expandable thumbnail

    Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963)

    Negative control: liver (PMID: 10607391), spleen (PMID: 10607391).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Rat hippocampus tissue lysate at 20 µg

    Lane 6: Rat brain tissue lysate at 20 µg

    Lane 7: Rat spleen tissue lysate at 20 µg

    Lane 8: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa

    Exposure time: 26s

  • Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963), expandable thumbnail

    Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963)

    Negative control: liver (PMID: 10607391), spleen (PMID: 10607391).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (ab316963) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human hippocampus tissue lysate at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Lane 4: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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