Rabbit Recombinant Monoclonal ARHGEF9 antibody. Carrier free. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430083.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)")
![Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--immunoprecipitation-img430182.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)")
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430082.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)")
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430207.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)")
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430206.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).
Alternative names
ARHDH9, KIAA0424, ARHGEF9, Rho guanine nucleotide exchange factor 9, Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9
Recommended products
Rabbit Recombinant Monoclonal ARHGEF9 antibody. Carrier free. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28024-32
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316964 is the carrier-free version of Anti-Collybistin antibody [EPR28024-32] ab316963.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collybistin also known as ARHGEF9 is a protein that functions as a guanine nucleotide exchange factor (GEF) primarily for the small GTPase Cdc42. This mechanism is essential in the regulation of actin cytoskeleton dynamics with an approximate molecular mass of 46 kDa. You can find Collybistin mainly in the brain where it plays a significant role in synaptic formation and function particularly in the postsynaptic sites of inhibitory synapses.
- Biological function summary
Collybistin plays a critical role in clustering and trafficking of specific proteins required for synapse maturation and stability. It participates as a part of a larger complex that includes key synaptic proteins like Gephyrin a scaffolding protein important for neurotransmitter receptor localization. This interaction between Collybistin and synaptic components ensures proper synaptic inhibitory signaling by controlling receptor clustering and distribution.
- Pathways
Collybistin is essential within the pathways associated with synaptic vesicle function and cytoskeletal dynamics. This protein interacts with important pathways like the Rho GTPase signaling pathway and Cdc42 effector pathways which are vital for cytoskeletal rearrangements. Through these pathways Collybistin engages with proteins such as F-actin influencing synaptic plasticity and neural connectivity by altering actin dynamics.
- Associated diseases and disorders
Collybistin is linked to neurological conditions with particular relevance to epilepsy. Mutations in the Collybistin encoding gene ARHGEF9 have been associated with forms of epilepsy and intellectual disabilities. In these conditions disrupted interactions between Collybistin and other synaptic proteins like Gephyrin can lead to altered synaptic signaling illustrating the importance of its proper function in neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430083.jpg "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)")
Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)
This data was developed using Anti-Collybistin antibody [EPR28024-32] ab316963, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with human ARHG4.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (Anti-Collybistin antibody [EPR28024-32] ab316963) at 1/1000 dilution
Lane 1: His-tagged human Collybistin fragment at 10 ng with NFDM/TBST
Lane 2: His-tagged human ARHG4 recombinant protein at 10 ng with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 43 kDa
Exposure time: 6s
![Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--immunoprecipitation-img430182.jpg "Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)")
Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)
This data was developed using Anti-Collybistin antibody [EPR28024-32] ab316963, the same antibody clone in a different buffer formulation.
Collybistin was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-Collybistin antibody [EPR28024-32] ab316963 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Collybistin antibody [EPR28024-32] ab316963 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2: Anti-Collybistin antibody [EPR28024-32] ab316963 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Collybistin antibody [EPR28024-32] ab316963 in SH-SY5Y whole cell lysateAll lanes: Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (Anti-Collybistin antibody [EPR28024-32] ab316963) at 1/30 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 84s
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430082.jpg "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)")
Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)
This data was developed using Anti-Collybistin antibody [EPR28024-32] ab316963, the same antibody clone in a different buffer formulation.
Negative control: HUVEC (PMID: 19911011).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (Anti-Collybistin antibody [EPR28024-32] ab316963) at 1/1000 dilution
Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg with NFDM/TBST
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg with NFDM/TBST
Lane 3: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 40 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa
Exposure time: 70s
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430207.jpg "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)")
Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)
This data was developed using Anti-Collybistin antibody [EPR28024-32] ab316963, the same antibody clone in a different buffer formulation.
Negative control: liver (PMID: 10607391), spleen (PMID: 10607391).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (Anti-Collybistin antibody [EPR28024-32] ab316963) at 1/1000 dilution
Lane 1: Mouse hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 2: Mouse brain tissue lysate at 20 µg with NFDM/TBST
Lane 3: Mouse spleen tissue lysate at 20 µg with NFDM/TBST
Lane 4: Mouse liver tissue lysate at 20 µg with NFDM/TBST
Lane 5: Rat hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 6: Rat brain tissue lysate at 20 µg with NFDM/TBST
Lane 7: Rat spleen tissue lysate at 20 µg with NFDM/TBST
Lane 8: Rat liver tissue lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964), expandable thumbnail](https://content.abcam.com/products/images/collybistin-antibody-epr28024-32-bsa-and-azide-free-ab316964--western-blot-img430206.jpg "Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)")
Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (ab316964)
This data was developed using Anti-Collybistin antibody [EPR28024-32] ab316963, the same antibody clone in a different buffer formulation.
Negative control: liver (PMID: 10607391), spleen (PMID: 10607391).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10607391).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Collybistin antibody [EPR28024-32] (Anti-Collybistin antibody [EPR28024-32] ab316963) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with NFDM/TBST
Lane 4: Human spleen tissue lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 49 kDa, 55 kDa, 61 kDa, 36 kDa
Exposure time: 26s
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