Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)

This data was developed using ab316963, the same antibody clone in a different buffer formulation.

Collybistin was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab316963 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316963 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : ab316963 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316963 in SH-SY5Y whole cell lysate

All lanes:

Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (<a href='/en-us/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 84s

• WB

Supplier Data

Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)

This data was developed using ab316963, the same antibody clone in a different buffer formulation.

Negative control : HUVEC (PMID : 19911011).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10607391).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Collybistin antibody [EPR28024-32] (<a href='/en-us/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 49 kDa,55 kDa,61 kDa,36 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)

This data was developed using ab316963, the same antibody clone in a different buffer formulation.

Negative control : liver (PMID : 10607391), spleen (PMID : 10607391).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10607391).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Collybistin antibody [EPR28024-32] (<a href='/en-us/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 49 kDa,55 kDa,61 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)

This data was developed using ab316963, the same antibody clone in a different buffer formulation.

Negative control : liver (PMID : 10607391), spleen (PMID : 10607391).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10607391).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Collybistin antibody [EPR28024-32] (<a href='/en-us/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Rat hippocampus tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Lane 8:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 49 kDa,55 kDa,61 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Collybistin antibody [EPR28024-32] - BSA and Azide free (AB316964)

This data was developed using ab316963, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with human ARHG4.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Collybistin antibody [EPR28024-32] (<a href='/en-us/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/1000 dilution

Lane 1:

His-tagged human Collybistin fragment at 10 ng

Lane 2:

His-tagged human ARHG4 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 43 kDa

false

Exposure time: 6s