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AB317859

Anti-Collybistin antibody [EPR28024-40] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ARHGEF9 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

ARHDH9, KIAA0424, ARHGEF9, Rho guanine nucleotide exchange factor 9, Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9

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Sandwich ELISA - Anti-Collybistin antibody [EPR28024-40] - BSA and Azide free (Detector) (AB317859)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Collybistin antibody [EPR28024-40] - BSA and Azide free (Detector) (AB317859)

Sandwich ELISA of ab317857 with the capture antibody ab317858 dilution at 2 ug/mL and detector antibody ab317859 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28024-40

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

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Reactivity data

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Product details

ab317859 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Collybistin. The recommended pair for sandwich ELISA is:

Capture: ab317858, Collybistin Capture Antibody (unconjugated)

Detector: ab317859, Collybistin Detector Antibody (unconjugated)

The reference range value is 0.16-10 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collybistin also known as ARHGEF9 is a protein that functions as a guanine nucleotide exchange factor (GEF) primarily for the small GTPase Cdc42. This mechanism is essential in the regulation of actin cytoskeleton dynamics with an approximate molecular mass of 46 kDa. You can find Collybistin mainly in the brain where it plays a significant role in synaptic formation and function particularly in the postsynaptic sites of inhibitory synapses.
Biological function summary

Collybistin plays a critical role in clustering and trafficking of specific proteins required for synapse maturation and stability. It participates as a part of a larger complex that includes key synaptic proteins like Gephyrin a scaffolding protein important for neurotransmitter receptor localization. This interaction between Collybistin and synaptic components ensures proper synaptic inhibitory signaling by controlling receptor clustering and distribution.

Pathways

Collybistin is essential within the pathways associated with synaptic vesicle function and cytoskeletal dynamics. This protein interacts with important pathways like the Rho GTPase signaling pathway and Cdc42 effector pathways which are vital for cytoskeletal rearrangements. Through these pathways Collybistin engages with proteins such as F-actin influencing synaptic plasticity and neural connectivity by altering actin dynamics.

Collybistin is linked to neurological conditions with particular relevance to epilepsy. Mutations in the Collybistin encoding gene ARHGEF9 have been associated with forms of epilepsy and intellectual disabilities. In these conditions disrupted interactions between Collybistin and other synaptic proteins like Gephyrin can lead to altered synaptic signaling illustrating the importance of its proper function in neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).
See full target information ARHGEF9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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