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Rabbit Polyclonal COMMD1/MURR1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human COMMD1.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human COMMD1. Database link Q8N668
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/2000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/5000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Target data

Function

Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141). Negatively regulates neuroblastoma G1/S phase cell cycle progression and cell proliferation by stimulating ubiquitination of NF-kappa-B subunit RELA and NF-kappa-B degradation in a FAM107A- and actin-dependent manner (PubMed:28604741).

Alternative names

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Rabbit Polyclonal COMMD1/MURR1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human COMMD1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human COMMD1. Database link Q8N668
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Western blot - Anti-COMMD1/MURR1 antibody (ab224727), expandable thumbnail

    Western blot - Anti-COMMD1/MURR1 antibody (ab224727)

    All lanes: Western blot - Anti-COMMD1/MURR1 antibody (AB224727) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma cell line) whole cell lysate

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lane 5: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunoprecipitation - Anti-COMMD1/MURR1 antibody (ab224727), expandable thumbnail

    Immunoprecipitation - Anti-COMMD1/MURR1 antibody (ab224727)

    COMMD1/MURR1 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab224727 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab224727 at 1/1000 dilution. HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.

    Lane 1: Rabbit monoclonal IgG instead of ab224727 in HepG2 whole cell lysate.

    Lane 2: ab224727 IP in HepG2 whole cell lysate.

    Lane 3: HepG2 whole cell lysate 20 μg (Input).

    All lanes: Immunoprecipitation - Anti-COMMD1/MURR1 antibody (AB224727)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMMD1/MURR1 antibody (ab224727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMMD1/MURR1 antibody (ab224727)

    Paraffin-embedded human liver cancer tissue stained for COMMD1/MURR1 with ab224727 at 1/20 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMMD1/MURR1 antibody (ab224727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMMD1/MURR1 antibody (ab224727)

    Paraffin-embedded human adrenal gland tissue stained for COMMD1/MURR1 with ab224727 at 1/20 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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