Rabbit Polyclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications.
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- American journal of physiology. Lung cellular and molecular physiology 323:L400-L4092022Endothelin-1 depletion of cartilage oligomeric matrix protein modulates pulmonary artery superoxide and iron metabolism-associated mitochondrial heme biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yu,Norah Alruwaili,Melissa R Kelly,Bin Zhang,Aijing Liu,Yingqi Wang,Dong Sun,Michael S WolinPubMed 35943724
- Scientific reports 11:46902021Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Miyazaki,Tatsuya Oda,Yuki Inagaki,Hiroko Kushige,Yutaka Saito,Nobuhito Mori,Yuzo Takayama,Yutaro Kumagai,Toutai Mitsuyama,Yasuyuki S KidaPubMed 33633222
- Cell cycle (Georgetown, Tex.) 20:96-1112021Over-expression of MEG3 promotes differentiation of bone marrow mesenchymal stem cells into chondrocytes by regulating miR-129-5p/RUNX1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zhu,Qiwei Fu,Jiahua Shao,Jinhui Peng,Qirong Qian,Yiqin Zhou,Yi ChenPubMed 33410373
- FEBS open bio 10:1856-18672020ADAMTS7 degrades Comp to fuel BMP2-dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Wang et. al.PubMed 32692461
- Clinical proteomics 16:342019Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong Beom Ahn et. al.PubMed 31467500
- Biomaterials 222:1194562019Chiral geometry regulates stem cell fate and activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingqing Dong et. al.PubMed 31476662
- American journal of physiology. Lung cellular and 317:L569-L5772019Potential role of cartilage oligomeric matrix protein in the modulation of pulmonary arterial smooth muscle superoxide by hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yu et. al.PubMed 31389735
- American journal of translational research 11:2232-22442019MicroRNA-495 enhances chondrocyte apoptosis, senescence and promotes the progression of osteoarthritis by targeting AKT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingyu Zhao et. al.PubMed 31105831
- Equine veterinary journal 51:787-7942019The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double-blinded, placebo-controlled proof-of-concept study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Y Broeckx et. al.PubMed 30815897
- Nature 562:133-1392018Discovery of a periosteal stem cell mediating intramembranous bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShawon Debnath et. al.PubMed 30250253
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
Alternative names
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5
Recommended products
Rabbit Polyclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.
- Biological function summary
COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.
- Pathways
COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.
- Associated diseases and disorders
COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab74524 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406
All lanes: Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524) at 1 µg/mL
Lane 1: Human skeletal muscle tissue lysate at 10 µg
Lane 2: Human heart tissue lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 100 kDa
Exposure time: 20min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524)
COMP/Cartilage oligomeric matrix protein western blot using anti-COMP/Cartilage oligomeric matrix protein antibody ab74524. Publication image and figure legend from Wang, C., Chen, Y., et al., 2020, FEBS Open Bio, PubMed 32692461.
ab74524 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74524 please see the product overview.
Comp was degraded by ADAMTS7 in OS cells. (A) Western blot of Comp protein levels in OS tissues and adjacent tissues (n = 26). P value by Student's t‐test The relative expression level was quantified. (B) Protein expression levels are presented as the log2 (fold‐change)in OS samples relative to the adjacent tissues. (C) Correlation of ADAMTS7 and Comp protein in OS tissues. (D) Representative images of western blot for full‐length Comp and degradation fragment in MG63 cells after ADAMTS7 overexpressing or silencing (n = 3).
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