Anti-COMP/Cartilage oligomeric matrix protein antibody

• WB

Lab

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (AB74524)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab74524 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa

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Exposure time: 20min

• WB

CiteAb

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (AB74524)

COMP/Cartilage oligomeric matrix protein western blot using anti-COMP/Cartilage oligomeric matrix protein antibody ab74524. Publication image and figure legend from Wang, C., Chen, Y., et al., 2020, FEBS Open Bio, PubMed 32692461.

ab74524 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74524 please see the product overview.

Comp was degraded by ADAMTS7 in OS cells. (A) Western blot of Comp protein levels in OS tissues and adjacent tissues (n = 26). P value by Student's t‐test The relative expression level was quantified. (B) Protein expression levels are presented as the log2 (fold‐change)in OS samples relative to the adjacent tissues. (C) Correlation of ADAMTS7 and Comp protein in OS tissues. (D) Representative images of western blot for full‐length Comp and degradation fragment in MG63 cells after ADAMTS7 overexpressing or silencing (n = 3).

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