AB260037

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]

Name: Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) | Abcam
Brand: Abcam Limited
SKU: AB260037
Price: 635 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5

4 Images

Unknown

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg human articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human articular cartilage lysate 10ug

Lane 2 : ab260037 IP in human articular cartilage lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260037 in human articular cartilage lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Predicted band size: 82 kDa

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Unknown

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg mouse articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse articular cartilage lysate 10ug

Lane 2 : ab260037 IP in mouse articular cartilage lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260037 in mouse articular cartilage lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Predicted band size: 82 kDa

false

Lab

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9143347, 24917676).

Negative control : Jurkat (Database : EBI Expression Atlas).

Exposure time : Lanes 1-2 : 1 second; Lane 3 : 125 seconds.

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution

Lane 1:

Human articular cartilage lysate at 20 µg

Lane 2:

Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

Lane 3:

Mouse articular cartilage lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa,250 kDa

false

Lab

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9143347, 24917676).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution

All lanes:

Rat articular cartilage lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa

false

Carrier free

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22857-38

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.

Biological function summary

COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.

Pathways

COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.

COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).

See full target information COMP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 35:e22848 PubMed34328678

2021

LINC00511 facilitates Temozolomide resistance of glioblastoma cells via sponging miR-126-5p and activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,Meng Tian,Jiongbo Liu,Kuanhong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (AB260037)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise