Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
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(2 Publications)
Rabbit Recombinant Monoclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
View Alternative Names
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human skeletal muscle (PMID : 19808781). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human liver (PMID : 14748877). The section was incubated with ab300555 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Human skeletal muscle tissue 10 ug with ab300555 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lanes 1 - 2:
Human skeletal muscle tissue lysate
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300555 in Human skeletal muscle tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 76s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of paraffin-embedded Mouse cartilage tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cartilage (PMID : 33668140, PMID : 16542502 ).The section was incubated with ab300555 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat liver (PMID : 14748877). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on mouse liver (PMID : 14748877). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse liver (PMID : 14748877). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse skeletal muscle (PMID : 19808781). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Mouse articular cartilage tissue lysate 10 ug with ab300555 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lanes 1 - 2:
Mouse articular cartilage tissue lysate
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300555 in mouse articular cartilage tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID : 24917676). The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID : 9143347).
All lanes:
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/5000 dilution
All lanes:
Human articar cartilage tissue lysate 20
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution
Observed band size: 100 kDa,82 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)
Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID : 24917676). The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID : 9143347).Negative control : liver (PMID : 14748877)
All lanes:
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lane 1:
Mouse articar cartilage tissue lysate 20
Lane 2:
Mouse liver tissue lysate
Lane 3:
Rat articar cartilage tissue lysate 20
Lane 4:
Rat liver tissue lysate
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Observed band size: 100 kDa,82 kDa
false
Exposure time: 15s
Related conjugates and formulations (3)
-
Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (BSA and Azide free)
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.
Pathways
COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.
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Target data
Publications (2)
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Cell proliferation 58:e13818 PubMed39902627
2025
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Unspecified application
Species
Unspecified reactive species
Cancer letters 600:217180 PubMed39154702
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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