Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)

Rabbit Recombinant Monoclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	ICC/IF	Flow Cyt (Intra)	IP
Human	Tested	Tested	Expected	Not recommended	Not recommended	Tested
Mouse	Tested	Tested	Tested	Not recommended	Not recommended	Tested
Rat	Tested	Not recommended	Tested	Not recommended	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Not Applicable.
Species Rat	Dilution info 1/500	Notes Not Applicable.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information COMP

Function

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).

Alternative names

Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5

Recommended products

Rabbit Recombinant Monoclonal COMP/Cartilage oligomeric matrix protein antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25364-6
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.

Biological function summary

COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.

Pathways

COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.

Associated diseases and disorders

COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 24917676).
The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID: 9143347).Negative control: liver (PMID: 14748877)
All lanes: Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lane 1: Mouse articar cartilage tissue lysate 20
Lane 2: Mouse liver tissue lysate
Lane 3: Rat articar cartilage tissue lysate 20
Lane 4: Rat liver tissue lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 100 kDa, 82 kDa
Exposure time: 15s
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 24917676).
The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID: 9143347).
All lanes: Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/5000 dilution
All lanes: Human articar cartilage tissue lysate 20
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution
Observed band size: 100 kDa, 82 kDa
Exposure time: 15s
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat liver (PMID: 14748877). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse liver (PMID: 14748877). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
.
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Mouse articular cartilage tissue lysate 10 ug with ab300555 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse articular cartilage tissue lysate 10 ug
Lane 2: abAB300555 IP in Mouse articular cartilage tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300555 in mouse articular cartilage tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds
All lanes: Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lanes 1 - 2: Mouse articular cartilage tissue lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 6s
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Human skeletal muscle tissue lysate 10 ug with ab300555 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human skeletal muscle tissue lysate 10 ug
Lane 2: abAB300555 IP in Human skeletal muscle tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300555 in human skeletal muscle tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds
All lanes: Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution
Lanes 1 - 2: Human skeletal muscle tissue lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 76s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on human liver (PMID: 14748877). The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human skeletal muscle (PMID: 19808781). The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on mouse liver (PMID: 14748877). The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse skeletal muscle (PMID: 19808781). The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555)
Immunohistochemical analysis of paraffin-embedded Mouse cartilage tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cartilage (PMID: 33668140, PMID: 16542502 ).The section was incubated with ab300555 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins