AB273626

Anti-Complement C8 antibody [EPR23646-22]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CO8A antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

2 Images

Lab

Western blot - Anti-Complement C8 antibody [EPR23646-22] (AB273626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-Complement C8 antibody [EPR23646-22] (ab273626) at 1/1000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

false

Lab

Western blot - Anti-Complement C8 antibody [EPR23646-22] (AB273626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 23869880).

Low expression : HEK-293 (Database : EBI-Expression Atlas, HPA).

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-Complement C8 antibody [EPR23646-22] (ab273626) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 ( human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

false

Carrier free

Anti-Complement C8 antibody [EPR23646-22] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23646-22

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody specifically recognizes the Complement component C8 alpha chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Complement C8 protein plays an important role in the immune response specifically as a component of the membrane attack complex (MAC). Known also simply as C8 it is composed of three subunits: C8α C8β and C8γ with a combined molecular mass of approximately 151 kDa. C8 is synthesized primarily by liver hepatocytes but its expression occurs in several other tissues such as monocytes and macrophages highlighting its significance in immune surveillance.

Biological function summary

The membrane attack complex is a pivotal element of the complement system where Complement C8 serves as a final assembly participant. It collaborates with other proteins such as C5b C6 and C7 to penetrate and lyse target cell membranes protecting the host against pathogenic invaders. C8 forms part of a larger complement cascade which activates sequentially ensuring swift response to microbial threats. This process highlights its relevance in innate immunity.

Pathways

Several significant biological processes involve Complement C8. The complement system and the coagulation pathway both rely on this protein's function. Within these pathways C8 associates closely with proteins such as C9 in forming the lytic pore of the MAC and C3b which facilitates the opsonization and clearance of pathogens. These collaborative actions support efficient immune defense and maintain homeostatic balance within the bloodstream.

Complement system dysregulation can link to immune complex diseases such as systemic lupus erythematosus (SLE) and paroxysmal nocturnal hemoglobinuria (PNH). In SLE improper complement activation possibly involving Complement C8 may lead to the formation of immune complexes that cause inflammation and tissue damage. In PNH the lack of complement inhibitory proteins results in red blood cell destruction where the unregulated MAC formation involving C8 plays a part in the hemolytic process. Understanding these connections helps unravel the complex roles that Complement C8 has in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.

See full target information C8A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106417 PubMed37153444

2023

Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zelinger,Tammy M Martin,Jayshree Advani,Laura Campello,Milton A English,Alan Kwong,Claire Weber,Jennifer Maykoski,Yuri V Sergeev,Robert Fariss,Emily Y Chew,Michael L Klein,Anand Swaroop

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Complement C8 antibody [EPR23646-22]

Western blot - Anti-Complement C8 antibody [EPR23646-22] (AB273626)

Western blot - Anti-Complement C8 antibody [EPR23646-22] (AB273626)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise