Rabbit Recombinant Monoclonal CO8A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector) (AB285135), expandable thumbnail](https://content.abcam.com/products/images/complement-c8a-antibody-epr23646-33-bsa-and-azide-free-detector-ab285135--sandwich-elisa-img185552.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector) (AB285135)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
Alternative names
Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A
Recommended products
Rabbit Recombinant Monoclonal CO8A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23646-33
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab285135 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Complement C8a (C8A). The recommended pair for sandwich ELISA is:
Capture: Anti-Complement C8a antibody [EPR23646-142] - BSA and Azide free (Capture) ab285029, Human Complement C8a (C8A) Capture Antibody (unconjugated)
Detector: ab285135, Human Complement C8a (C8A) Detector Antibody (unconjugated)
The reference range value is 62 - 2000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement C8a also known as C8 alpha chain is a part of the complement system which plays a role in immune response. It has a molecular mass of approximately 64 kDa. This protein is found in blood plasma and is mainly expressed in hepatocytes. The C8a forms the terminal subunit of the C8 protein complex which is critical for the formation of the membrane attack complex (MAC) that disrupts the lipid bilayer of target cells.
- Biological function summary
The C8a chain works in concert with C8 beta and C8 gamma to form the C8 complex of the complement system. This protein becomes part of the larger MAC a lytic complex that facilitates cell membrane disruptions thereby initiating cell lysis. The membrane attack complex plays a defensive role against pathogenic microorganisms contributing to innate immunity.
- Pathways
The complement system involving C8a plays a central role in the innate immune pathway and the alternative pathway of complement activation. These pathways are essential for the protection against infections. The C5 convertase acts upstream of C8a cleaving C5 to C5b which then associates with C6 and C7 allowing C8a and its counterparts C8b and C8g to bind and initiate MAC formation. C9 is the final protein to bind completing the complex and resulting in the lysis of the target.
- Associated diseases and disorders
The deficiency or dysfunction in C8a can be associated with increased susceptibility to Neisseria infections particularly meningococcal disease. C8 deficiency is often linked with deficiencies in C5-C9 proteins as these proteins are collectively critical for functional MAC formation. Immunological responses depending on the formation of the MAC can become compromised increasing vulnerability to certain bacterial infections.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector) (ab285135), expandable thumbnail](https://content.abcam.com/products/images/complement-c8a-antibody-epr23646-33-bsa-and-azide-free-detector-ab285135--sandwich-elisa-img185552.jpg "Sandwich ELISA - Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector) (ab285135)")
Sandwich ELISA - Anti-Complement C8a antibody [EPR23646-33] - BSA and Azide free (Detector) (ab285135)
Example of Human C8a standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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