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AB281050

Anti-Complement factor 8 beta/C8B antibody [EPR23764-8] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CO8B antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Complement component C8 beta chain, Complement component 8 subunit beta, C8B

1 Images
Sandwich ELISA - Anti-Complement factor 8 beta/C8B antibody [EPR23764-8] - BSA and Azide free (Capture) (AB281050)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Complement factor 8 beta/C8B antibody [EPR23764-8] - BSA and Azide free (Capture) (AB281050)

Example of Human C8b standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23764-8

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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Primary Antibodies

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Primary Antibodies

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Western blot - Anti-C3 antibody [EPR19394] (AB200999)

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Primary Antibodies

AB309149

Anti-Complement factor B antibody [EPR27443-6]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor B antibody [EPR27443-6] (AB309149)

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Primary Antibodies

AB275931

Anti-C5 / C5b antibody [EPR23940-3]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281050 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human C8B. The recommended pair for sandwich ELISA is:
Capture: ab281200, Human C8B Capture Antibody (unconjugated)
Detector: ab281050, Human C8B Detector Antibody (unconjugated)
The reference range value is 0.04 - 10 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor 8 beta (C8B) is an essential protein component of the complement system an important part of the immune response. Commonly referred to as C8B this protein has a molecular mass of approximately 64 kilodaltons. C8B is primarily expressed in liver tissue where it contributes to the formation of the C8 protein complex a component necessary for the assembly of the membrane attack complex (MAC). Other names for this target include complement component C8 beta chain emphasizing its role in the complement cascade.
Biological function summary

The C8B protein facilitates the stabilization of the C8 complex which also consists of the alpha and gamma chains to perform its role in the immune system. It plays an important role in forming the MAC an important structure for lysing pathogenic cells like bacteria by disrupting their plasma membranes. This action highlights the importance of C8B in innate immunity where it enables the host to eliminate harmful cells effectively.

Pathways

C8B's activities are integral to the complement cascade specifically within the terminal membrane attack pathway. It functions in conjunction with other complement factors like complement factor 5 to 9 forming a cascade of sequential activations. C8B works closely with complement factor 8 alpha and complement factor C5 within this pathway to achieve effective immune responses. These interactions ensure the proper function of the immune surveillance mechanism allowing the organism to protect itself against microbial infections.

C8B has significant connections to immunodeficiencies and inflammatory conditions. Patients with deficiencies in any of the components forming the MAC including C8B often suffer from recurrent Neisserial infections due to impaired bacterial lysis. C8B mutations contribute to properdin-independent pathway issues that can lead to susceptibility to such infections. Moreover malfunction in C8B can associate with autoimmune disorders implying a disruption in the delicate balance of the immune response where proteins like factor 8 and complement factor 5 play critical roles in maintaining immunity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 7440581). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 30552328, PubMed : 39914456, PubMed : 39814882, PubMed : 7440581). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 30552328, PubMed : 7440581). C8B, together with C8A and C8G, inserts into the target membrane, but does not form pores by itself (PubMed : 30552328). During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity (PubMed : 30552328, PubMed : 6833260).
See full target information C8B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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