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AB133765

Anti-Complement factor B antibody [EPR9288(B)]

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(3 Publications)

Rabbit Recombinant Monoclonal CFAB antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

2 Images
Western blot - Anti-Complement factor B antibody [EPR9288(B)] (AB133765)
  • WB

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Western blot - Anti-Complement factor B antibody [EPR9288(B)] (AB133765)

All lanes:

Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 86 kDa

Observed band size: 60 kDa,95 kDa

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OI-RD Scanning - Anti-Complement factor B antibody [EPR9288(B)] (AB133765)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Complement factor B antibody [EPR9288(B)] (AB133765)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Complement factor B antibody [EPR9288(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9288(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor B also known as CFB protein is a component of the complement system. It plays an important role in the alternative pathway. This protein with a molecular mass of approximately 93 kDa is produced mainly in the liver and expresses in various tissues throughout the body. Factor B comprises several domains including the von Willebrand factor type A domain essential for its activity. CFB engages in protein-protein interactions important for the function of the complement cascade.
Biological function summary

Complement factor B is essential in the immune system. It participates in the formation of the C3 convertase complex which is an important step in the amplification of the complement cascade. This protein contributes to the opsonization and clearance of pathogens assisting in the immune response. CFB binds with complement component C3b to participate in the alternative pathway which does not require antibody presence for activation.

Pathways

Complement factor B is part of the innate immune pathway. It helps mediate the alternative complement pathway acting as an amplifier of immune responses. This pathway interacts closely with the classical and lectin pathways ensuring a versatile defense mechanism. Factor D for example also plays a complementary role by cleaving factor B when bound to C3b to form C3 convertase a critical step in propagating the immune response.

Complement factor B associates with kidney-related diseases like atypical hemolytic uremic syndrome (aHUS). Mutations in CFB can lead to deregulated complement activation contributing to pathological conditions like age-related macular degeneration (AMD). The relationship with protein C3 is important in these disorders as both factor B and C3 engage in interactions that when dysfunctional influence disease outcomes. Understanding these interactions can help develop targeted therapies such as anti-complement agents to modulate the complement system in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
See full target information CFB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35163494

2022

Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Joëlle Al-Choboq,Mélanie L Ferlazzo,Laurène Sonzogni,Adeline Granzotto,Laura El-Nachef,Mira Maalouf,Elise Berthel,Nicolas Foray

Oncology letters 21:478 PubMed33968194

2021

Low CFB expression is independently associated with poor overall and disease-free survival in patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglu He,Ya Li,Ruixian Zhang,Jing Chen,Xingxing Feng,Yong Duan

PloS one 14:e0222403 PubMed31568522

2019

Multiple biomarkers of sepsis identified by novel time-lapse proteomics of patient serum.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhiro Hayashi,Syunta Yamaguchi,Frans Rodenburg,Sing Ying Wong,Kei Ujimoto,Takahiro Miki,Toshiaki Iba
View all publications
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Product promise

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