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Rabbit Recombinant Monoclonal CFAB antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Sandwich ELISA - Anti-Complement Factor B (Ba fragment) antibody [EPR25032-130] - BSA and Azide free (Capture) (AB316818), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Alternative names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

Recommended products

Rabbit Recombinant Monoclonal CFAB antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25032-130
Purification technique
Affinity purification Protein A
Specificity

This antibody does not cross-react with full-length human Factor B.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316818 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Complement Factor B (Ba fragment). The recommended pair for sandwich ELISA is:

Capture: ab316818, Human Complement Factor B (Ba fragment) Capture Antibody (unconjugated)

Detector: Anti-Complement Factor B (Ba fragment) antibody [EPR24245-52] - BSA and Azide free (Detector) ab316819, Human Complement Factor B (Ba fragment) Detector Antibody (unconjugated)

The reference range value is 0.16-20 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Complement Factor B also known as CFB Ba fragment or C3 proactivator participates mechanically in the alternative complement pathway. It has a molecular mass of approximately 93 kDa. Expressed mainly in the liver and found in plasma Factor B cleaves into two fragments Bb and Ba by Factor D. The Ba fragment specifically is known for its interactions that modulate complement activation. Ba itself although smaller is an active part of this cleavage event.

Biological function summary

Complement Factor B is important in the innate immune response. It forms a complex called the C3 convertase when combined with C3b and Bb fragment which amplifies the cascade leading to pathogen opsonization and lysis. The Ba fragment though not part of the convertase influences cell signaling and modulates inflammatory responses. It directly affects immune cells by binding to receptors and therefore impacts the regulation of immune responses.

Pathways

The alternative complement pathway and the regulation of inflammation fall under the influence of Complement Factor B. Within the complement pathway its interaction with proteins like C3 and Factor D emphasizes its position. This pathway is part of wider immune management processes highlighting its key role interacting with these proteins to control activation and amplification of the complement system leading to enhanced pathogen clearance and aiding the defense mechanism.

Associated diseases and disorders

Complement Factor B shows connections to age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). In AMD abnormal regulation of the complement pathway with involvement of Complement Factor B contributes to drusen formation complicating the disease progression. It also links to aHUS where uncontrolled complement activation causes endothelial damage. Proteins such as Complement Factor H which modulates the alternative pathway show a significant relationship with Factor B in these disease processes affecting the progression and control of these disorders through complement regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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