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Mouse Monoclonal Complex I Immunocapture antibody. Suitable for IP, ICC/IF, Flow Cyt and reacts with Mouse, Human, Cow, Rat samples. Cited in 17 publications.

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Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFFlow Cyt
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Expected
Expected
Expected
Cow
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

0.10000-1.00000 mg/mL

Notes

100 μg mAb can capture at least 25 μg complex I from 1 mg solubilized bovine heart mitochondria.

Species

Human

Dilution info

0.10000-1.00000 mg/mL

Notes

100 μg mAb can capture at least 25 μg complex I from 1 mg solubilized bovine heart mitochondria.

Species

Cow

Dilution info

0.10000-1.00000 mg/mL

Notes

100 μg mAb can capture at least 25 μg complex I from 1 mg solubilized bovine heart mitochondria.

Expected
Expected

Species

Rat

Dilution info

0.10000-1.00000 mg/mL

Notes

100 μg mAb can capture at least 25 μg complex I from 1 mg solubilized bovine heart mitochondria.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Cow, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Recommended products

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Mouse Monoclonal Complex I Immunocapture antibody. Suitable for IP, ICC/IF, Flow Cyt and reacts with Mouse, Human, Cow, Rat samples. Cited in 17 publications.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Clone number

18G12BC2

Purity

IgG fraction

Light chain type

kappa

Concentration
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Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798), expandable thumbnail

    Immunoprecipitation - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)

    Complex I was immunopurified from mitochondria isolated from human heart (HHM), cow/bovine heart (BHM), mouse heart (MHM) and mouse brain (MBM). The lanes were stained with Coomassie Brilliant Blue R. Bands were excised from the gel and proteolytically digested for mass spectrometry analysis. For the immuno-isolation, 50 μg of mAb (18G12BC2 ab109798) was bound to 5 μl of swollen protein G agarose beads according to protocol described here.

    All lanes: Immunoprecipitation - Anti-Complex I Immunocapture antibody [18G12BC2] (AB109798)

  • Flow Cytometry - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798), expandable thumbnail

    Flow Cytometry - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)

    HL-60 cells were stained with 1 µg/mL Complex I antibody ab109798 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  • Immunocytochemistry/ Immunofluorescence - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)

    Immunocytochemistry image of ab109798 stained fibroblasts cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab109798, 1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 4884 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.

  • Flow Cytometry - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798), expandable thumbnail

    Flow Cytometry - Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)

    Overlay histogram showing HepG2 cells stained with ab109798 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109798, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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