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AB109865

Anti-Complex II Immunocapture antibody [4H12BG12AG2]

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(13 Publications)

Mouse Monoclonal Complex II antibody. Suitable for IP, ICC/IF and reacts with Human, Cow samples. Cited in 13 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (AB109865)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (AB109865)

ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (AB109865)
  • IP

Unknown

Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (AB109865)

Complex II immunoprecipitation using antibody ab109865 crosslinked to protein G-agarose beads. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria in lane 1 and 500 ug human heart mitochondria in lane 2. The gel was stained with coomassie brilliant blue.

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Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4H12BG12AG2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Cow, Human

Applications

IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
ab109865 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex II also known as succinate dehydrogenase (SDH) is an important enzyme in the mitochondrial electron transport chain. It plays a central role in cellular respiration by enabling the oxidation of succinate to fumarate while simultaneously transferring electrons to ubiquinone. Complex II weighs approximately 124 kDa and consists of four subunits: SDHA SDHB SDHC and SDHD. It is located on the inner mitochondrial membrane and is widely expressed in various tissues reflecting its fundamental role in energy metabolism.
Biological function summary

Complex II is integral to both the citric acid cycle and the electron transport chain. It acts as a bridge linking these two metabolic pathways. As part of this dual association Complex II helps maintain mitochondrial respiratory efficiency. Unlike other complexes it does not directly pump protons across the mitochondrial membrane; instead it focuses on electron transfer. This specific function highlights its importance in cellular energy metabolism.

Pathways

Complex II contributes to the tricarboxylic acid cycle (TCA cycle) and the oxidative phosphorylation pathway. These pathways are important for ATP production within cells. Complex II's relationship with proteins such as ubiquinone and cytochrome c highlights its role in electron transfer efficiency. As electrons pass through Complex II they are directed towards further complexes in the electron transport chain ultimately leading to energy production.

Mutations in Complex II subunits have been linked to disorders like paraganglioma and Leigh syndrome. These conditions often involve a disruption in energy metabolism within cells. The malfunction of related proteins like fumarase and other complexes can lead to a build-up of metabolites that result in pathogenesis. Understanding the function and regulation of Complex II can therefore provide insights into these mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Complex II
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2869 PubMed38693144

2024

A patient-based iPSC-derived hepatocyte model of alcohol-associated cirrhosis reveals bioenergetic insights into disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bani Mukhopadhyay,Cheryl Marietta,Pei-Hong Shen,Abdul Oiseni,Faridoddin Mirshahi,Maria Mazzu,Colin Hodgkinson,Eli Winkler,Qiaoping Yuan,Daniel Miranda,George Kunos,Arun J Sanyal,David Goldman

Nature communications 15:2553 PubMed38519472

2024

SNX8 enables lysosome reformation and reverses lysosomal storage disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Li,Cong Xiang,Shilei Zhu,Jiansheng Guo,Chang Liu,Ailian Wang,Jin Cao,Yan Lu,Dante Neculai,Pinglong Xu,Xin-Hua Feng

Molecular psychiatry 26:2721-2739 PubMed33664474

2021

The translocator protein (TSPO) is prodromal to mitophagy loss in neurotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Frison,Danilo Faccenda,Rosella Abeti,Manuel Rigon,Daniela Strobbe,Britannie S England-Rendon,Diana Cash,Katy Barnes,Mona Sadeghian,Marija Sajic,Lisa A Wells,Dong Xia,Paola Giunti,Kenneth Smith,Heather Mortiboys,Federico E Turkheimer,Michelangelo Campanella

Journal of molecular histology : PubMed33559814

2021

LGR4 silence aggravates ischemic injury by modulating mitochondrial function and oxidative stress via ERK signaling pathway in H9c2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Xiangrui Qiao,Lele Cheng,Mengping Liu,Yangyang Deng,Xiaozhen Zhuo

The Journal of biological chemistry 292:10239-10249 PubMed28458255

2017

Lysine desuccinylase SIRT5 binds to cardiolipin and regulates the electron transport chain.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxun Zhang,Sivakama S Bharathi,Matthew J Rardin,Jie Lu,Katherine V Maringer,Sunder Sims-Lucas,Edward V Prochownik,Bradford W Gibson,Eric S Goetzman

Skeletal muscle 6:30 PubMed27597886

2016

Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Iori Sakakibara,Maud Wurmser,Matthieu Dos Santos,Marc Santolini,Serge Ducommun,Romain Davaze,Anthony Guernec,Kei Sakamoto,Pascal Maire

Brain : a journal of neurology 136:1544-54 PubMed23599390

2013

A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

An I Jonckheere,G Herma Renkema,Maaike Bras,Lambert P van den Heuvel,Alexander Hoischen,Christian Gilissen,Sander B Nabuurs,Martijn A Huynen,Maaike C de Vries,Jan A M Smeitink,Richard J T Rodenburg

The journal of maternal-fetal & neonatal medicine 26:150-4 PubMed22928498

2012

Effects of labor on placental fatty acid β oxidation.

Applications

Unspecified application

Species

Human

Hector Mendez-Figueroa,Edward K Chien,Huiling Ji,Nicole L Nesbitt,Sivakama S Bharathi,Eric Goetzman

PloS one 6:e23295 PubMed21858060

2011

Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia W S Finley,Wilhelm Haas,Valérie Desquiret-Dumas,Douglas C Wallace,Vincent Procaccio,Steven P Gygi,Marcia C Haigis

The Journal of nutrition 141:603-10 PubMed21346097

2011

PPARalpha expression protects male mice from high fat-induced nonalcoholic fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Abdelmegeed,Seong-Ho Yoo,Lauren E Henderson,Frank J Gonzalez,Kimberley J Woodcroft,Byoung-Joon Song
View all publications
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