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Mouse Monoclonal UQCRFS1/RISP antibody. Suitable for IP and reacts with Human, Cow samples. Cited in 9 publications.

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Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (AB109862), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Human
Tested
Cow
Tested

Tested
Tested

Species

Human

Dilution info

100 µg/mg of lysate

Notes

-

Species

Cow

Dilution info

100 µg/mg of lysate

Notes

-

Target data

Function

Cytochrome b-c1 complex subunit Rieske, mitochondrialComponent of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).Cytochrome b-c1 complex subunit 9Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS) (PubMed:8386158, PubMed:2996928). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (By similarity).

Alternative names

Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Rieske protein UQCRFS1, Ubiquinol-cytochrome c reductase iron-sulfur subunit, RISP, UQCRFS1

Recommended products

Mouse Monoclonal UQCRFS1/RISP antibody. Suitable for IP and reacts with Human, Cow samples. Cited in 9 publications.

Alternative names

Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Rieske protein UQCRFS1, Ubiquinol-cytochrome c reductase iron-sulfur subunit, RISP, UQCRFS1

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

11A51H12

Specificity

100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.

Light chain type

kappa

Concentration
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Purification notes

ab10862 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Complex III also known as cytochrome bc1 complex or ubiquinol-cytochrome c reductase is an important component of the mitochondrial electron transport chain. This protein complex has a mass of approximately 500 kDa and is located in the inner mitochondrial membrane. Complex III plays a mechanical role by facilitating the transfer of electrons from ubiquinol to cytochrome c while simultaneously pumping protons across the mitochondrial membrane. This action helps in establishing the electrochemical gradient used for ATP synthesis.

Biological function summary

The molecular actions of Complex III are integral to aerobic respiration. It forms part of the larger mitochondrial respiratory chain complex working closely with Complexes I II and IV. Complex III through its electron transport and proton translocation activities helps in the production of ATP an energy currency vital for cellular processes. It also influences the generation of reactive oxygen species (ROS) which can serve as cellular signals or cause damage under certain conditions.

Pathways

Complex III is embedded in the oxidative phosphorylation pathway as a central component. It interacts with other protein complexes like Complex I and IV which are also important for the production of ATP. By transferring electrons it plays a role in maintaining the efficiency of the electron transport chain. Even slight dysfunctions in Complex III's activities can lead to disruptions in the oxidative phosphorylation pathway affecting overall cellular energy metabolism.

Associated diseases and disorders

Deficiencies or mutations in Complex III correlate with mitochondrial diseases and neurodegenerative disorders. For example Complex III dysfunction has been connected with diseases such as mitochondrial myopathy and Parkinson's disease. In such conditions the protein's activity directly impacts ATP production and ROS levels linking it to disruptions in cellular homeostasis. Mutations in its associated proteins like cytochrome c can exacerbate these disorders highlighting Complex III's significance in maintaining physiological function.

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1 product image

  • Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862), expandable thumbnail

    Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

    Staining of Complex III
    Lane 1: 0.1mg of Bovine mitochondria
    Lane 2: 0.05mg Bovine mitochondria
    Lane 3: 0.05mg Human mitochondria
    Immunoprecipitated using ab109862 at 10 µg/mg lysate.

    All lanes: Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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