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AB109863

Anti-Complex IV Immunocapture antibody [31E91B82G9]

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(1 Review)

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(4 Publications)

Mouse Monoclonal MTCO1 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human, Cow samples. Cited in 4 publications.

View Alternative Names

COI, COXI, MTCO1, MT-CO1, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)

ICC/IF image of ab109863 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109863, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Flow Cytometry - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)
  • Flow Cyt

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Flow Cytometry - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)

Overlay histogram showing HepG2 cells stained with ab109863 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109863, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunoprecipitation - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)
  • IP

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Immunoprecipitation - Anti-Complex IV Immunocapture antibody [31E91B82G9] (AB109863)

Staining of Complex IV Lane 1 : 0.1 mg Human mitochondrial lysate Lane 2 : 0.1 mg Bovine mitochondrial lysate Lane 3 : 0.025 mg Human mitochondrial lysate Lane 4 : 0.025 mg Bovine mitochondrial lysate Immunoprecipitated using ab109863 at 100 µg/mg lysate

All lanes:

Immunoprecipitation - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

31E91B82G9

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Cow, Human

Applications

ICC/IF, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

100 µg ab109863 can capture at least 10 µg Complex IV (COX) from 1 mg solubilized Bovine heart mitochondria.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "100 µg/mg of lysate", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "100 µg/mg of lysate", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
ab109863 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex IV also known as cytochrome c oxidase is a multi-subunit enzyme complex important for the electron transport chain (ETC) in mitochondria. It consists of 13 protein subunits and has an approximate mass of 204 kDa. Complex IV is located in the inner mitochondrial membrane where it facilitates the transfer of electrons from cytochrome c to molecular oxygen reducing it to water. This process helps create a proton gradient across the inner membrane which is essential for ATP production. In high-energy demand tissues like heart and skeletal muscle expression levels of Complex IV are notably higher.
Biological function summary

Complex IV plays a critical role in cellular respiration by being the terminal enzyme of the ETC. It is part of a larger complex and its primary function is to aid in oxidative phosphorylation. This complex facilitates oxygen consumption in cells aiding ATP synthesis and influencing metabolic rate. Complex IV is involved in maintaining cellular energy homeostasis and regulation of reactive oxygen species which also contributes to cell signaling and apoptosis.

Pathways

Complex IV takes a vital role in the oxidative phosphorylation and apoptosis pathways. In oxidative phosphorylation it interacts with other ETC complexes including Complex I and II to assure efficient ATP production. The role of Complex IV in apoptosis pathways involves interactions with cytochrome c which upon release into the cytosol can trigger the apoptotic machinery. These pathways are fundamental for cellular energy production and programmed cell death ensuring proper cell function and turnover.

Complex IV dysfunction is associated with mitochondrial diseases and neurodegenerative disorders. Mitochondrial diseases such as Leigh syndrome can occur due to mutations affecting Complex IV subunits leading to impaired energy production and metabolic acidosis. In neurodegenerative disorders like Alzheimer's disease altered Complex IV activity contributes to energy deficits and increased oxidative stress. Proteins like amyloid-beta have shown connections with Complex IV alterations in Alzheimer's disease hinting at a link between mitochondrial dysfunction and the pathogenesis of such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information MT-CO1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13368 PubMed33951271

2021

Gain of PITRM1 peptidase in cortical neurons affords protection of mitochondrial and synaptic function in an advanced age mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Du,Qing Yu,Shijun Yan,Zhihua Zhang,Jhansi Rani Vangavaragu,Doris Chen,Shi Fang Yan,Shirley ShiDu Yan

The Journal of biological chemistry 295:2544-2554 PubMed31974161

2020

Pulse-chase SILAC-based analyses reveal selective oversynthesis and rapid turnover of mitochondrial protein components of respiratory complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel F Bogenhagen,John D Haley

Molecular and cellular biology 30:5099-109 PubMed20805355

2010

Inhibition of human peptide deformylase disrupts mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Sindy Escobar-Alvarez,Jeffrey Gardner,Aneesh Sheth,Giovanni Manfredi,Guangli Yang,Ouathek Ouerfelli,Mark L Heaney,David A Scheinberg

Toxicology in vitro : an international journal pub 21:902-11 PubMed17346924

2007

Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays.

Applications

Unspecified application

Species

Unspecified reactive species

Sashi Nadanaciva,Autumn Bernal,Robert Aggeler,Roderick Capaldi,Yvonne Will
View all publications
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