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AB126618

Anti-COMT antibody [EPR6490]

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(1 Review)

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(10 Publications)

Rabbit Recombinant Monoclonal COMT antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 10 publications.

View Alternative Names

Catechol O-methyltransferase, COMT

3 Images
Western blot - Anti-COMT antibody [EPR6490] (AB126618)
  • WB

Lab

Western blot - Anti-COMT antibody [EPR6490] (AB126618)

Lanes 1 - 4 : Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

Lane 1:

Wild type HAP1 whole cell lysate at 40 µg

Lane 2:

COMT knockout HAP1 whole cell lysate at 40 µg

Lane 3:

A431 whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 30 kDa

false

Western blot - Anti-COMT antibody [EPR6490] (AB126618)
  • WB

Lab

Western blot - Anti-COMT antibody [EPR6490] (AB126618)

Lanes 1-2 : Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.

ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

COMT knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human COMT knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-comt-knockout-hek-293t-cell-line-ab266537'>ab266537</a>)

Predicted band size: 30 kDa

Observed band size: 24-28 kDa

false

Western blot - Anti-COMT antibody [EPR6490] (AB126618)
  • WB

Unknown

Western blot - Anti-COMT antibody [EPR6490] (AB126618)

All lanes:

Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Lane 4:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

false

  • Carrier free

    Anti-COMT antibody [EPR6490] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6490

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COMT also known as catechol-O-methyltransferase is an enzyme responsible for the methylation of catechol compounds including neurotransmitters like dopamine epinephrine and norepinephrine. This reaction requires S-adenosylmethionine as a methyl donor. COMT primarily has two forms: a soluble form and a membrane-bound form with masses around 24 kDa and 30 kDa respectively. This protein shows significant expression in the liver brain and kidneys. COMT expression is also noted in cell lines such as HEK293T where it plays an important role in catecholamine metabolism.
Biological function summary

Catechol-O-methyltransferase serves as a regulator of neurotransmitter levels in the central nervous system and peripheral tissues. It does not appear to form part of a larger protein complex functioning mainly as a singular entity for metabolizing catechols. Through its action COMT impacts processes such as mood regulation cognition and stress response. Its role in degrading dopamine is especially significant in regions like the prefrontal cortex where dopamine plays an important role in executive functions.

Pathways

COMT participates in the catecholamine degradation pathway and impacts dopaminergic signaling. It works alongside enzymes such as monoamine oxidase (MAO) to regulate levels of neurotransmitters. COMT's activity influences the synaptic presence of dopamine and its interaction in signaling pathways. Moreover it indirectly affects the balance within the dopaminergic system which is important for normal neurological function and behavior.

COMT has links to psychiatric and neurological conditions such as schizophrenia and Parkinson’s disease. Variations in COMT function can affect dopamine metabolism which may contribute to the etiology of these disorders. Additionally proteins like MAO and dopamine transporter (DAT) also connect to COMT through their shared involvement in neurotransmitter regulation. Alterations in COMT activity can influence disease severity and patient response to treatment underlining its importance in understanding these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
See full target information Catechol O-methyltransferase
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:2906-2927 PubMed40195499

2025

The selenocysteine-containing protein SELENOT maintains dopamine signaling in the midbrain to protect mice from hyperactivity disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Guo,Zhao-Feng Li,Dong-Yan Hu,Pei-Jun Li,Kai-Nian Wu,Hui-Hui Fan,Jie Deng,Hong-Mei Wu,Xiong Zhang,Jian-Hong Zhu

Communications biology 6:1155 PubMed37957344

2023

SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Valsecchi,Francesco Errico,Valentina Bassareo,Carmen Marino,Tommaso Nuzzo,Paola Brancaccio,Giusy Laudati,Antonella Casamassa,Manuela Grimaldi,Adele D'Amico,Manolo Carta,Enrico Bertini,Giuseppe Pignataro,Anna Maria D'Ursi,Alessandro Usiello

Evidence-based complementary and alternative medicine : eCAM 2022:9191284 PubMed36437828

2022

Therapeutic and Neuroprotective Effects of Bushen Jianpi Decoction on a Rotenone-Induced Rat Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liang,Lulu Yao,Jiahao Chen,Zhiying Chen,Xiling Wu,Xiaobo Huang

Pharmacology 107:81-89 PubMed34794150

2021

Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Harisa Ferizovic,Natasa Spasojevic,Milica Jankovic,Bojana Stefanovic,Sladjana Dronjak

Science translational medicine 13: PubMed34011628

2021

Gene therapy restores dopamine transporter expression and ameliorates pathology in iPSC and mouse models of infantile parkinsonism.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne Ng,Serena Barral,Carmen De La Fuente Barrigon,Gabriele Lignani,Fatma A Erdem,Rebecca Wallings,Riccardo Privolizzi,Giada Rossignoli,Haya Alrashidi,Sonja Heasman,Esther Meyer,Adeline Ngoh,Simon Pope,Rajvinder Karda,Dany Perocheau,Julien Baruteau,Natalie Suff,Juan Antinao Diaz,Stephanie Schorge,Jane Vowles,Lucy R Marshall,Sally A Cowley,Sonja Sucic,Michael Freissmuth,John R Counsell,Richard Wade-Martins,Simon J R Heales,Ahad A Rahim,Maximilien Bencze,Simon N Waddington,Manju A Kurian

Frontiers in aging neuroscience 9:410 PubMed29311899

2017

Metabolism of Dopamine in Nucleus Accumbens Astrocytes Is Preserved in Aged Mice Exposed to MPTP.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany M Winner,Harue Zhang,McKenzie M Farthing,Lalitha M Karchalla,Keith J Lookingland,John L Goudreau

Veterinary pathology 54:851-854 PubMed28578627

2017

Coati Bodies: Cytoplasmic Hyaline Globules in the Ganglionic Neurons of Aging Captive Coatis.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy K Cooper,Michael M Garner,Jennifer Baccon,Haresh Mani

Nature communications 7:12733 PubMed27681312

2016

FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase.

Applications

Unspecified application

Species

Unspecified reactive species

Khanh V Doan,Ann W Kinyua,Dong Joo Yang,Chang Mann Ko,Sang Hyun Moh,Ko Eun Shong,Hail Kim,Sang-Kyu Park,Dong-Hoon Kim,Inki Kim,Ji-Hye Paik,Ronald A DePinho,Seul Gi Yoon,Il Yong Kim,Je Kyung Seong,Yun-Hee Choi,Ki Woo Kim

European neuropsychopharmacology : the journal of 26:1629-37 PubMed27499503

2016

Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels.

Applications

WB

Species

Rat

Bojana Stefanovic,Natasa Spasojevic,Predrag Jovanovic,Nebojsa Jasnic,Jelena Djordjevic,Sladjana Dronjak

Progress in neuro-psychopharmacology & biological psychiatry 56:149-54 PubMed25194460

2014

Perinatal asphyxia alters neuregulin-1 and COMT gene expression in the medial prefrontal cortex in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoyasu Wakuda,Keiko Iwata,Yasuhide Iwata,Ayyappan Anitha,Taro Takahashi,Kohei Yamada,Mahesh Mundalil Vasu,Hideo Matsuzaki,Katsuaki Suzuki,Norio Mori
View all publications
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