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Rabbit Recombinant Monoclonal COMT antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 7 publications.


Images

Western blot - Anti-COMT antibody [EPR6490] (AB126618), expandable thumbnail
  • Western blot - Anti-COMT antibody [EPR6490] (AB126618), expandable thumbnail
  • Western blot - Anti-COMT antibody [EPR6490] (AB126618), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Expected
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Alternative names

Catechol O-methyltransferase, COMT

Recommended products

Rabbit Recombinant Monoclonal COMT antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6490
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COMT also known as catechol-O-methyltransferase is an enzyme responsible for the methylation of catechol compounds including neurotransmitters like dopamine epinephrine and norepinephrine. This reaction requires S-adenosylmethionine as a methyl donor. COMT primarily has two forms: a soluble form and a membrane-bound form with masses around 24 kDa and 30 kDa respectively. This protein shows significant expression in the liver brain and kidneys. COMT expression is also noted in cell lines such as HEK293T where it plays an important role in catecholamine metabolism.

Biological function summary

Catechol-O-methyltransferase serves as a regulator of neurotransmitter levels in the central nervous system and peripheral tissues. It does not appear to form part of a larger protein complex functioning mainly as a singular entity for metabolizing catechols. Through its action COMT impacts processes such as mood regulation cognition and stress response. Its role in degrading dopamine is especially significant in regions like the prefrontal cortex where dopamine plays an important role in executive functions.

Pathways

COMT participates in the catecholamine degradation pathway and impacts dopaminergic signaling. It works alongside enzymes such as monoamine oxidase (MAO) to regulate levels of neurotransmitters. COMT's activity influences the synaptic presence of dopamine and its interaction in signaling pathways. Moreover it indirectly affects the balance within the dopaminergic system which is important for normal neurological function and behavior.

Associated diseases and disorders

COMT has links to psychiatric and neurological conditions such as schizophrenia and Parkinson’s disease. Variations in COMT function can affect dopamine metabolism which may contribute to the etiology of these disorders. Additionally proteins like MAO and dopamine transporter (DAT) also connect to COMT through their shared involvement in neurotransmitter regulation. Alterations in COMT activity can influence disease severity and patient response to treatment underlining its importance in understanding these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618), expandable thumbnail

    Western blot - Anti-COMT antibody [EPR6490] (ab126618)

    All lanes: Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: Rat spleen lysate at 20 µg

    Lane 4: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618), expandable thumbnail

    Western blot - Anti-COMT antibody [EPR6490] (ab126618)

    Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human COMT knockout HEK-293T cell line ab266537 (knockout cell lysate Human COMT knockout HEK-293T cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: COMT knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human COMT knockout HEK-293T cell line (Human COMT knockout HEK-293T cell line ab266537)

    Performed under reducing conditions.

    Predicted band size: 30 kDa

    Observed band size: 24-28 kDa

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618), expandable thumbnail

    Western blot - Anti-COMT antibody [EPR6490] (ab126618)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1: Wild type HAP1 whole cell lysate at 40 µg

    Lane 2: COMT knockout HAP1 whole cell lysate at 40 µg

    Lane 3: A431 whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Predicted band size: 30 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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