Browse all our conjugated antibodies

What are conjugated antibodies?

Conjugated antibodies are antibodies that are chemically linked to a detectable label, such as a fluorescent dye, enzyme, or nanoparticle. This direct linkage enables immediate visualization or measurement of target antigens without requiring a secondary antibody step.

Conjugated antibodies are primary antibodies pre-coupled with detection molecules, enabling direct antigen detection across multiple assay formats. These reagents simplify workflows and improve reproducibility by eliminating additional labeling or validation steps typically required in indirect detection methods.

Our portfolio includes 60,000+ validated primary antibodies supported by over 600,000 citations. These tools provide consistent performance, reduce experimental variability, and streamline workflows, helping researchers achieve reliable, high-quality results while saving time in demanding laboratory environments.

Why choose our conjugated antibodies?

Highly validated

Highly validated antibodies are reagents that have been tested across multiple experimental applications to ensure consistent binding and performance. Our conjugated antibodies undergo validation in flow cytometry, ICC/IF, IHC/IF, and western blot, providing confidence in specificity and reproducibility across common research techniques.

Consistent batches

Consistent antibody batches refer to standardized production processes that minimize variability between lots. Our quality assurance systems ensure high signal-to-noise ratios by controlling conjugation efficiency and removing free dye, resulting in cleaner, more reliable experimental outcomes.

Time saving

Time-saving reagents are tools designed to reduce preparation and validation steps in experimental workflows. Conjugated antibodies eliminate the need for in-house conjugation and validation, enabling researchers to focus on data generation and analysis rather than reagent preparation.

Quicker assay

Quicker assays are workflows that reduce the number of experimental steps and incubation times. By eliminating the need for secondary antibodies, conjugated primary antibodies streamline assay protocols and workflows, particularly in high-throughput or time-sensitive applications.

Reduced background noise

Reduced background noise refers to minimizing non-specific signals that can obscure true results. Direct detection eliminates secondary antibody cross-reactivity, lowering the risk of false positives and improving overall assay specificity.

Custom antibody conjugation

Custom antibody conjugation is a service that tailors antibody labeling to specific experimental requirements. Expert-driven optimization ensures the appropriate label, conjugation chemistry, and performance characteristics for specialized or multiplex assays.

We offer a wide range of labels, including:

• Alexa Fluor® dyes: 405, 488, 532, 555, 568, 594, 647, 680, 600, 750, 790
• Phycoerythrin (PE), allophycocyanin (APC), fluorescein isothiocyanate (FITC)
• Biotin, horseradish peroxidase (HRP), alkaline phosphatase (AP)
• Gold nanoparticles (10, 20, 40 or 80 nm)
• Oligonucleotide/DNA barcodes

Conjugated antibodies allow for direct detection of target antigens in various assay formats. By coupling antibodies with a detection molecule, you can readily visualize the presence and quantity of specific proteins without secondary antibodies. These conjugates are essential in applications ranging from western blotting and immunohistochemistry to enzyme-linked immunosorbent assays (ELISA) and flow cytometry.

Types of conjugated antibodies

HRP conjugated antibodies

HRP-conjugated antibodies are antibodies linked to horseradish peroxidase, an enzyme used for signal generation in immunoassays. These antibodies produce chemiluminescent or colorimetric signals and are widely used in western blotting and ELISA due to their high sensitivity.

Alexa Fluor® conjugated antibodies

Alexa Fluor® conjugated antibodies are antibodies labeled with highly stable fluorescent dyes. These dyes provide strong brightness and photostability, making them ideal for fluorescence microscopy and flow cytometry, particularly in experiments requiring long imaging times or multiplex detection.

PE conjugated antibodies

PE-conjugated antibodies are antibodies linked to phycoerythrin, a highly bright fluorescent protein. These conjugates are commonly used in flow cytometry, where their intense fluorescence enables sensitive detection of low-abundance targets.

Other conjugated antibodies

Other conjugated antibodies refer to antibodies labeled with alternative detection molecules, including FITC, APC, AP, and biotin. These options offer flexibility in assay design, supporting a wide range of detection systems from enzymatic assays to multiplex fluorescence analysis.

Applications of conjugated antibodies

References

1. Griffiths, G. & Lucocq, J. Antibodies for immunolabeling by light and electron microscopy: not for the faint-hearted. Histochem. Cell Biol. 142, 347–360 (2014).
2. Sule, R., Rivera, G. & Gomes, A. V. Western blotting (immunoblotting): history, theory, uses, protocol, and problems. Biotechniques 75, 99–114 (2023).
3. Mishra, M. et al. Improving the sensitivity of traditional Western blotting via streptavidin-containing poly-horseradish peroxidase (PolyHRP). Electrophoresis 40, 1731–1739 (2019).
4. Flor, A. et al. DNA-directed assembly of antibody–fluorophore conjugates for quantitative multiparametric flow cytometry. ChemBioChem 15, 267–275 (2014).