Phycoerythrin (PE) conjugated antibodies are known for their intense brightness, making them ideal for detecting low-abundance proteins and rare cell populations in flow cytometry.

Key features

Brightness

PE stands out with its exceptional brightness and broader Stokes shift, making it an excellent choice for detecting low-abundance targets.

Compatibility with multi-color experiments

Due to their peak excitation at 565 nm and emission at 578 nm, PE conjugated antibodies can be effectively combined with other fluorophores for multi-color flow cytometry panels without significant spectral overlap.

Stability

PE-conjugated antibodies are very stable, enabling consistent performance across experiments.

FAQ

What are PE-conjugated antibodies used for?

PE-conjugated antibodies are ideal for identifying and quantifying different cell populations in flow cytometry. PE's bright fluorescence allows for clear resolution of positive and negative populations, even when paneling multiple fluorophores, making this conjugate ideal for high-resolution flow cytometry, especially in complex or dim samples.

Can PE-conjugated antibodies be used with other fluorescent dyes in flow cytometry?

Yes, PE-conjugated antibodies can be combined with other fluorophores that have distinct excitation and emission spectra, making them ideal for multi-color flow cytometry panels.

Are PE-conjugated antibodies suitable for quantitative assays?

Yes, the intense brightness and stability of PE make these antibodies suitable for both qualitative and quantitative fluorescence-based assays.