JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224721

Anti-Connecdenn 3 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Connecdenn 3 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DENND1C aa 600 to C-terminus.

View Alternative Names

FAM31C, DENND1C, DENN domain-containing protein 1C, Connecdenn 3, Protein FAM31C

3 Images
Western blot - Anti-Connecdenn 3 antibody (AB224721)
  • WB

Supplier Data

Western blot - Anti-Connecdenn 3 antibody (AB224721)

All lanes:

Western blot - Anti-Connecdenn 3 antibody (ab224721) at 1/500 dilution

Lane 1:

Rat liver lysate

Lane 2:

Rat lung lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 87 kDa

Observed band size: 88 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (AB224721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (AB224721)

Paraffin-embedded human lymph node tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (AB224721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (AB224721)

Paraffin-embedded human lung tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DENND1C aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IV53

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connecdenn 3 also known as DENND1C is a guanine nucleotide exchange factor with a mass of approximately 125 kDa. It plays an important role in cell signaling by activating small GTPases. The protein is primarily expressed in human immune cells with significant presence in dendritic cells. It has a part in modulating endocytic trafficking impacting vesicle formation and cargo sorting.
Biological function summary

This protein is part of the DENN domain proteins family known to regulate endosomal functions. Connecdenn 3 participates in the process of endocytosis facilitating the movement of materials into the cell. Through its role as a guanine nucleotide exchange factor it affects the activation of Rab small GTPases which are essential for intracellular transport and recycling of membrane components.

Pathways

Connecdenn 3 functions centrally in endocytic and recycling pathways. It collaborates with Rab proteins important for cargo selection and vesicle budding. Through these interactions it influences the membrane trafficking machinery and cellular signaling networks connected to the movement of vesicles. It has a relationship with the Ras-related protein Rab-5 impacting pathways associated with the transport and sorting of endocytic vesicles.

Connecdenn 3 links to immunological conditions and is associated with allergies. Altered expression levels or mutations in Connecdenn 3 can disrupt Rab GTPase activity affecting immune responses. The protein's relationship with Rab-5 suggests its involvement in disorders where vesicle trafficking and cellular communication are impaired potentially contributing to abnormal immune function or inflammatory processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
See full target information DENND1C
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com