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AB199178

Anti-Connexin 30.3 / GJB4 antibody

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Connexin 30.3 / GJB4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GJB4.

View Alternative Names

Gap junction beta-4 protein, Connexin-30.3, Cx30.3, GJB4

2 Images
Western blot - Anti-Connexin 30.3 / GJB4 antibody (AB199178)
  • WB

Supplier Data

Western blot - Anti-Connexin 30.3 / GJB4 antibody (AB199178)

Gel : 10%SDS-PAGE

All lanes:

Western blot - Anti-Connexin 30.3 / GJB4 antibody (ab199178) at 1/200 dilution

Lane 1:

Mouse skin tissue lysate at 40 µg

Lane 2:

HeLa cell lysate at 40 µg

Predicted band size: 30 kDa

true

Exposure time: 5min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 30.3 / GJB4 antibody (AB199178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 30.3 / GJB4 antibody (AB199178)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Connexin 30.3 / GJB4 with ab199178 at 1/40.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GJB4. The exact immunogen used to generate this antibody is proprietary information.

Q9NTQ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 30.3 also known as Gap Junction Beta 4 (GJB4) is a protein that forms gap junction channels important for cell communication. This protein allows direct transfer of ions and small molecules between adjacent cells creating an essential connection for maintaining tissue homeostasis. Connexin 30.3 has a molecular mass of approximately 31 kDa and is expressed in various tissues including skin and nervous tissue.
Biological function summary

Connexin 30.3 plays a role in cell signaling and helps in maintaining the integrity of cell layers. It assembles into hexameric complexes known as connexons which dock with connexons from neighboring cells to form functional gap junctions. These channels facilitate communication among cells essential for processes such as differentiation and homeostatic regulation.

Pathways

Connexin 30.3 has significant involvement in the regulation of the calcium signaling pathway working closely with other connexins like connexin 43. Interaction within the gap junction communication pathway impacts various cellular responses including metabolic cooperation between cells. These pathways ensure coordinated cellular activities significant for overall tissue health.

Mutations in Connexin 30.3 can lead to skin disorders such as erythrokeratodermia variabilis. This condition involves impaired cell communication and abnormal keratinization of the skin. Additionally connexin 30.3 relates to hearing disorders as it shares functional pathways with connexin 26 and other connexins known to influence auditory functions. Proper understanding of these interactions aids in exploring therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of gap junctions (By similarity). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (By similarity).
See full target information GJB4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 15:e0240129 PubMed33048975

2020

Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Ryuji Okamoto,Itaru Goto,Yuhei Nishimura,Issei Kobayashi,Ryotaro Hashizume,Yoshinori Yoshida,Rie Ito,Yuhko Kobayashi,Misato Nishikawa,Yusuf Ali,Shunsuke Saito,Toshio Tanaka,Yoshiki Sawa,Masaaki Ito,Kaoru Dohi

American journal of physiology. Gastrointestinal a 318:G803-G815 PubMed32116021

2020

Unraveling the transcriptional determinants of liver sinusoidal endothelial cell specialization.

Applications

Unspecified application

Species

Unspecified reactive species

Willeke de Haan,Cristina Øie,Mohammed Benkheil,Wouter Dheedene,Stefan Vinckier,Giulia Coppiello,Xabier López Aranguren,Manu Beerens,Joris Jaekers,Baki Topal,Catherine Verfaillie,Bård Smedsrød,Aernout Luttun
View all publications
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Product promise

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