AB256132

Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free

Recombinant
What is this?

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(1 Publication)

Mouse Recombinant Monoclonal Connexin 32 / GJB1 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

CX32, GJB1, Gap junction beta-1 protein, Connexin-32, GAP junction 28 kDa liver protein, Cx32

2 Images

Lab

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (AB256132)

The molecular observed was in consist with published literatures (PMID 11226030, PMID : 11245683, PMID : 12403817).

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).

All lanes:

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] (<a href='/en-us/products/primary-antibodies/connexin-32-gjb1-antibody-2a-ab78052'>ab78052</a>) at 1.318 µg/mL

All lanes:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 32 kDa

Observed band size: 100 kDa,46 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (AB256132)

The molecular observed was in consist with published literatures (PMID 11226030, PMID : 11245683, PMID : 12403817).

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).

All lanes:

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] (<a href='/en-us/products/primary-antibodies/connexin-32-gjb1-antibody-2a-ab78052'>ab78052</a>) at 1.318 µg/mL

Lane 1:

Human liver tissue lysate at 60 µg

Lane 2:

Mouse liver tissue lysate at 60 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 32 kDa

Observed band size: 100 kDa,46 kDa

false

Exposure time: 180s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab256132 is the carrier-free version of ab78052.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 32 also known as GJB1 is a gap junction protein with a molecular mass of approximately 32 kDa. This protein facilitates direct intercellular communication by forming gap junction channels that allow ions and small molecules to pass between adjacent cells. Connexin 32 is expressed in various tissues including liver pancreas and many types of epithelial cells. Its expression ensures cells can synchronize their activities important for maintaining tissue homeostasis.

Biological function summary

Connexin 32 plays a significant role in maintaining cellular communication networks. This protein forms hexameric complexes called connexons which dock with connexons of adjacent cells to create functional gap junction channels. These channels are essential in processes such as maintaining homeostasis cell differentiation and synchronized contraction of tissues like cardiac muscle. The structural formation allows direct transfer of metabolites and signaling molecules highlighting its importance in multicellular organism function.

Pathways

Connexin 32 actively contributes to cellular communication and metabolic coordination. It participates in the phosphoinositide 3-kinase (PI3-K) pathway and the ERK/MAPK signaling pathway which are important for cell growth proliferation and apoptosis. Connexin 32 interacts with proteins like Connexin 43 with both proteins playing roles in channel assembly and intracellular signaling processes that influence cellular responses to external stimuli.

Connexin 32's dysfunction aligns with specific neuropathies. Mutations in the GJB1 gene often lead to X-linked Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. This disease involves degeneration of nerve fibers disrupting muscle function and sensation and connects with proteins that share functional pathways like PMP22. Additionally Connexin 32 has implications in liver diseases where its reduced expression correlates with disrupted hepatocyte communication affecting liver function and regeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

See full target information GJB1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Reproduction (Cambridge, England) 121:77-88 PubMed11226030

2001

Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.

Applications

Unspecified application

Species

Unspecified reactive species

C S Wright,D L Becker,J S Lin,A E Warner,K Hardy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (AB256132)

Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (AB256132)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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